BBa_J23017 1 BBa_J23017 RLambda traG knockout (graveyarded) 2006-07-23T11:00:00Z 2015-08-31T04:08:39Z PCR JL8/JL9 on pKD3 (~1000bp, dPN1, Neb2) Electroporate into pKD46xRlambda R plasmid with traG knockout false false _52_ 0 363 52 No part sequence false JL8 Forward oligo to knockout traG-r: atgaagaaccgaaacaacgccgtggggccacagatacgggcgaaagtgCaggctggagctgcttcgaag JL9 Reverse oligo to knockout traG-r: tcatatcgtgatcccctccccttcctcgacggtggccgtctggattgggaaGtagccatggtccatatg false Will Bosworth igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z