LOCUS       BBa_J23018              2298 bp    DNA     linear       22-Nov-2024
DEFINITION  EN-Ptet-rbs-X-[RFP]-SNP "ORF Expresser"
ACCESSION   BBa_J23018
VERSION     BBa_J23018.1  
FEATURES             Location/Qualifiers
     misc_feature    2080..2208
                     /label=BBa_B0015 terminator
     misc_feature    2217..2270
                     /label=BBa_R0040 (Ptet)
     misc_feature    183..802
                     /label=pBR322 origin
     misc_feature    180..895
                     /label=pMB1 origin
     CDS             957..1817
                     /label=beta-lactamase (ampicillin resistance marker)
     promoter        1859..1887
                     /label=beta-lactamase (ampR) promoter
     stem_loop       2091..2134
                     /label=rrnB T1 terminator
     misc_feature    1..21
                     /label=BioBrick suffix
     misc_feature    2058..2063
                     /label=EcoRI
     misc_feature    2064..2071
                     /label=NotI
     misc_feature    2292..2297
                     /label=XbaI
     primer_bind     1940..1959
                     /label=Verification forward (VF2) primer binding site
     primer_bind     79..98
                     /label=Verification reverse (VR) primer binding site
     misc_feature    2279..2290
                     /label=BBa_B0034
     RBS             2279..2290
                     /label=RBS
     promoter        2217..2270
                     /label=Ptet
ORIGIN
        1 tactagtagc ggccgctgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
       61 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      121 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      181 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      241 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      301 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      361 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
      421 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
      481 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
      541 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
      601 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
      661 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
      721 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
      781 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
      841 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
      901 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
      961 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     1021 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     1081 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     1141 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     1201 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     1261 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     1321 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     1381 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     1441 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     1501 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     1561 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     1621 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     1681 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     1741 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     1801 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     1861 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     1921 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     1981 acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc
     2041 gctaaggatg atttctggaa ttcgcggccg caactagagc caggcatcaa ataaaacgaa
     2101 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     2161 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtccc
     2221 tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac tactagagaa
     2281 agaggagaaa ttctagag
//