##gff-version 3
##sequence-region BBa_J23018 1 2297
BBa_J23018	.	engineered_region	2080	2208	.	+	0	ID=annotation1893435;Name=BBa_B0015 terminator
BBa_J23018	.	sequence_feature	180	895	.	+	0	ID=annotation1934517;Name=pMB1 origin
BBa_J23018	.	sequence_feature	1	21	.	+	0	ID=annotation1975737;Name=BioBrick suffix
BBa_J23018	.	promoter	1859	1887	.	+	0	ID=annotation1975735;Name=beta-lactamase (ampR) promoter
BBa_J23018	.	sequence_feature	2058	2063	.	+	0	ID=annotation1975738;Name=EcoRI
BBa_J23018	.	sequence_feature	183	802	.	+	0	ID=annotation1893437;Name=pBR322 origin
BBa_J23018	.	sequence_feature	2292	2297	.	+	0	ID=annotation1975740;Name=XbaI
BBa_J23018	.	engineered_region	2279	2290	.	+	0	ID=annotation1975763;Name=BBa_B0034
BBa_J23018	.	stem_loop	2091	2134	.	+	0	ID=annotation1975736;Name=rrnB T1 terminator
BBa_J23018	.	CDS	957	1817	.	+	0	ID=annotation1975734;Name=beta-lactamase (ampicillin resistance marker)
BBa_J23018	.	promoter	2217	2270	.	+	0	ID=annotation1975765;Name=Ptet
BBa_J23018	.	primer_binding_site	1940	1959	.	+	0	ID=annotation1975760;Name=Verification forward (VF2) primer binding site
BBa_J23018	.	sequence_feature	2064	2071	.	+	0	ID=annotation1975739;Name=NotI
BBa_J23018	.	primer_binding_site	79	98	.	+	0	ID=annotation1975761;Name=Verification reverse (VR) primer binding site
BBa_J23018	.	ribosome_entry_site	2279	2290	.	+	0	ID=annotation1975764;Name=RBS
BBa_J23018	.	engineered_region	2217	2270	.	+	0	ID=annotation1893436;Name=BBa_R0040 (Ptet)
>BBa_J23018
tactagtagcggccgctgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcag
ctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagca
aaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaat
cgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgc
gctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatag
ctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagccc
gaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagcca
ctggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacac
tagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggc
aaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaag
atcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatc
aaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttgg
tctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgac
tccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacg
ctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatcc
gcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttg
ccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcg
agttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggcc
gcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtga
ctggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacggga
taataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatc
ttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcg
tttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcat
actcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttag
aaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatga
cattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgattt
ctggaattcgcggccgcaactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttta
tctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatact
agagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaat
tctagag