LOCUS       BBa_J23023               293 bp    DNA     linear       22-Nov-2024
DEFINITION  [Ptet][Key3][TT]
ACCESSION   BBa_J23023
VERSION     BBa_J23023.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    63..156
                     /label=BBa_J01086
     stem_loop       176..219
                     /label=stem_loop
     misc_feature    165..244
                     /label=BBa_B0010
     terminator      165..244
                     /label=BBa_B0010
     stem_loop       260..279
                     /label=T7 TE
     misc_feature    286..286
                     /label=stop
     polyA_site      280..293
                     /label=polya
     misc_feature    253..293
                     /label=BBa_B0012
     terminator      253..293
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agacccaaaa gcaggaggtg aatctagtag gtggttaatg aaaattaact tacttactag
      121 aaatatctct aaaaagccag attattaatc cggctttact agagccaggc atcaaataaa
      181 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
      241 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//