BBa_J23026 1 BBa_J23026 [TraG][TT] 2006-07-24T11:00:00Z 2015-08-31T04:08:39Z [TraGr][TT] Digest pSB1A2-J23013 (EcoRI/SpeI, Neb2, Small ) Paste into pSB1AKG0-B0015 (EcoRI/XbaI, Neb2, Large 2062) Product is pSB1A2-J23026. [TraG][TT] false false _52_ 0 483 95 Not in stock false n/a false John Anderson component1900888 1 BBa_B0010 component1900887 1 BBa_J23013 component1900890 1 BBa_B0012 annotation1900888 1 BBa_B0010 range1900888 1 1924 2003 annotation1900887 1 BBa_J23013 range1900887 1 1 1915 annotation1900890 1 BBa_B0012 range1900890 1 2012 2052 BBa_J23013 1 [TraGr] TraGr open reading frame basic part 2006-07-23T11:00:00Z 2015-08-31T04:08:39Z PCR JL10/JL11 on Rlambda (?bp, EcoRI/SpeI, Neb2) Paste into pSB1A2-I13521 (EcoRI/SpeI, Neb2, Large 2062) TraGr complement false false _52_ 0 363 52 Not in stock false JL10 Forward EcoRI oligo to bioBrick TraG: CCTAGGAATTCGCGGCCGCTTCTAGatgaagaaccgaaacaacgc JL11 Reverse SpeI oligo to bioBrick TraG: gacttACTAGTgaagcggctcatatcgtgatc false Will Bosworth BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_J23026_sequence 1 atgaagaaccgaaacaacgccgtggggccacagatacgggcgaaaaaaccgaaggccagcaagaccgttcccatcctcgccggcctgtcccttggggcaggcttgcagaccgcgacgcaatatttcgcccattccttccagtatcaggccggacttggctggaatatcaaccacgtctacacgccctggtcgatccttcaatgggccggcaagtggtacggccaatatcccgacgatttcatgcgcgcggccagcatgggtatggtcgtttcgaccgtgggcctgctgggcacggccgtcacgcagatggtcaaggcgaacaccggcaaggcgaacgactatctgcacggttcggcccgctgggccgacaagaaggacatacaggccgccggcctgctgccccggccgcgcaccgtcgtcgagctggtgtcaggcaaacacccgcccacttccagcggcgtctatgtgggcggctggcaggacaaggacggcaagttccactacctgcggcacaacggccccgagcacgtcttgacctacgcgccgacgcgctccggcaagggcgtcggcctggtcgttccgacgctgctttcctgggcgcacagcgccgtcatcaccgacctgaaaggcgagttgtgggcgctgaccgccggctggcggaagaagcatgcccgcaacaaggtcgtgcgctttgagccggcatccgcgcagggtagcgcgtgctggaacccgctcgatgaaatccgcttggggaccgagtacgaggttggcgacgtgcagaaccttgccaccctgatcgtcgatccggacggcaagggcctggaatcgcactggcagaaaaccagccaggcgctgcttgtcggcgtcatcctgcacgcgctctacaaggctaagaacgagggtacgccggccaccctgccgtcggtggacggcatgcttgccgacccgaaccgcgacgtgggcgagctttggatggaaatgaccacctacggccacgttgacgggcagaaccaccctgcggtcggctctgcggcccgcgacatgatggaccgcccggaggaagaatccggttccgtgctgtccaccgccaagtcctacctggccctgtaccgcgacccggttgtggcccgcaacgtcagcaagtccgacttccgcatcaagcaactgatgcaccacgacgacccggtaagcctgttcatcgtgacgcagcccaacgacaaggcccgtctgcggcctctggtgcgcgtcatggtcaacatgatcgtccggctgctggccgacaagatggacttcgagaacggccgtcctgtcgcgcactacaagcatcgtctgctgatgatgctcgacgagttccccagcctgggcaagctcgaaatcctgcaagagtcccttgccttcgtcgccggctacggcatcaagtgctacctcatctgccaggacattaaccagctcaagagccgcgaaaccggctacggccacgacgaaagcatcacgtcgaactgccacgtgcagaacgcctacccgccgaaccgcgtggagacggccgagcacctgtcaaaactgacaggcaccacgaccatcgtgaaggagcagatcacgacgagcggccgccgcacgtcggcgctgttgggcaacgtctcgcggaccttccaggaagtgcagcggccattgctgacccccgatgaatgcctgcgcatgccggggccgaagaagagcgccgacggcagcattgaagaggcgggcgacatggttgtgtacgtcgccggctatcccgcgatctacggcaagcagccgctctacttcaaagacccgatattccaggcccgcgcggccgtaccggctccgaaggtcagcgacaagctgatccagacggccaccgtcgaggaaggggaggggatcacgatatgagccgctttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J23013_sequence 1 atgaagaaccgaaacaacgccgtggggccacagatacgggcgaaaaaaccgaaggccagcaagaccgttcccatcctcgccggcctgtcccttggggcaggcttgcagaccgcgacgcaatatttcgcccattccttccagtatcaggccggacttggctggaatatcaaccacgtctacacgccctggtcgatccttcaatgggccggcaagtggtacggccaatatcccgacgatttcatgcgcgcggccagcatgggtatggtcgtttcgaccgtgggcctgctgggcacggccgtcacgcagatggtcaaggcgaacaccggcaaggcgaacgactatctgcacggttcggcccgctgggccgacaagaaggacatacaggccgccggcctgctgccccggccgcgcaccgtcgtcgagctggtgtcaggcaaacacccgcccacttccagcggcgtctatgtgggcggctggcaggacaaggacggcaagttccactacctgcggcacaacggccccgagcacgtcttgacctacgcgccgacgcgctccggcaagggcgtcggcctggtcgttccgacgctgctttcctgggcgcacagcgccgtcatcaccgacctgaaaggcgagttgtgggcgctgaccgccggctggcggaagaagcatgcccgcaacaaggtcgtgcgctttgagccggcatccgcgcagggtagcgcgtgctggaacccgctcgatgaaatccgcttggggaccgagtacgaggttggcgacgtgcagaaccttgccaccctgatcgtcgatccggacggcaagggcctggaatcgcactggcagaaaaccagccaggcgctgcttgtcggcgtcatcctgcacgcgctctacaaggctaagaacgagggtacgccggccaccctgccgtcggtggacggcatgcttgccgacccgaaccgcgacgtgggcgagctttggatggaaatgaccacctacggccacgttgacgggcagaaccaccctgcggtcggctctgcggcccgcgacatgatggaccgcccggaggaagaatccggttccgtgctgtccaccgccaagtcctacctggccctgtaccgcgacccggttgtggcccgcaacgtcagcaagtccgacttccgcatcaagcaactgatgcaccacgacgacccggtaagcctgttcatcgtgacgcagcccaacgacaaggcccgtctgcggcctctggtgcgcgtcatggtcaacatgatcgtccggctgctggccgacaagatggacttcgagaacggccgtcctgtcgcgcactacaagcatcgtctgctgatgatgctcgacgagttccccagcctgggcaagctcgaaatcctgcaagagtcccttgccttcgtcgccggctacggcatcaagtgctacctcatctgccaggacattaaccagctcaagagccgcgaaaccggctacggccacgacgaaagcatcacgtcgaactgccacgtgcagaacgcctacccgccgaaccgcgtggagacggccgagcacctgtcaaaactgacaggcaccacgaccatcgtgaaggagcagatcacgacgagcggccgccgcacgtcggcgctgttgggcaacgtctcgcggaccttccaggaagtgcagcggccattgctgacccccgatgaatgcctgcgcatgccggggccgaagaagagcgccgacggcagcattgaagaggcgggcgacatggttgtgtacgtcgccggctatcccgcgatctacggcaagcagccgctctacttcaaagacccgatattccaggcccgcgcggccgtaccggctccgaaggtcagcgacaagctgatccagacggccaccgtcgaggaaggggaggggatcacgatatgagccgctt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z