BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_J23014 1 [trbCf] trbCf open reading frame basic part 2006-07-23T11:00:00Z 2015-08-31T04:08:39Z PCR JL14/JL15 on Olambda (679bp, EcoRI/SpeI, Neb2) Paste into pSB1A2-I13521 (EcoRI/SpeI, Neb2, Large 2062) trbCf complement false false _52_ 0 363 52 Not in stock false Actually, oligos JL14 and JL15 did not work, so we had to create 20mers JL16 and JL17 in order to get the trbCf gene and will work with that. false Will Bosworth BBa_J23028 1 BBa_J23028 [trbCf][TT] 2006-07-24T11:00:00Z 2015-08-31T04:08:39Z [trbCf][TT] Digest pSB1A2-J23014 (EcoRI/SpeI, Neb2, Small ) Paste into pSB1AKG0-B0015 (EcoRI/XbaI, Neb2, Large 2062) Product is pSB1A2-J23028. [trbCf][TT] false false _52_ 0 483 95 Not in stock false n/a false John Anderson component1900895 1 BBa_B0010 component1900894 1 BBa_J23014 component1900897 1 BBa_B0012 annotation1900895 1 BBa_B0010 range1900895 1 651 730 annotation1900897 1 BBa_B0012 range1900897 1 739 779 annotation1900894 1 BBa_J23014 range1900894 1 1 642 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_J23014_sequence 1 atgaagctgagtatgaaatctctggcagcactgctgatgatgctgaacggggcggttatggcgtcagaaaacgtgaacactcctgaaaaccgccagttcctgaagcagcaggaaaatttaagcagacaactgcgtgaaaaacctgaccatcagctgaaagcctgggcggagaaacaggtgctggaaaacccccttcagcgttcagataaccatttcctggatgagctggttcgtaaacagcaggcttcgcaggacgggaaaccccggcagggtgctctgtattttgtgtcgttttccattcccgaagaggggctgaaacgaatgctgggcgaaacccggcacttcggtattccggccacactgcggggcatggtgaacaatgacctgaagaccaccgctgaagctgtgttgtccctggtgaaagacggcgcgactgatggtgttcagatcgacccgacgctgttttcacagtacggcattcgcactgtaccggcactggtggtgttctgcagtcagggatacgacatcatccggggaaacctgcgggtcggccaggcgctggagaaagtggccgccacgggtgactgccggcaggtggctcatgatttactggcagggaaaggagattccgggaaatgaaac BBa_J23028_sequence 1 atgaagctgagtatgaaatctctggcagcactgctgatgatgctgaacggggcggttatggcgtcagaaaacgtgaacactcctgaaaaccgccagttcctgaagcagcaggaaaatttaagcagacaactgcgtgaaaaacctgaccatcagctgaaagcctgggcggagaaacaggtgctggaaaacccccttcagcgttcagataaccatttcctggatgagctggttcgtaaacagcaggcttcgcaggacgggaaaccccggcagggtgctctgtattttgtgtcgttttccattcccgaagaggggctgaaacgaatgctgggcgaaacccggcacttcggtattccggccacactgcggggcatggtgaacaatgacctgaagaccaccgctgaagctgtgttgtccctggtgaaagacggcgcgactgatggtgttcagatcgacccgacgctgttttcacagtacggcattcgcactgtaccggcactggtggtgttctgcagtcagggatacgacatcatccggggaaacctgcgggtcggccaggcgctggagaaagtggccgccacgggtgactgccggcaggtggctcatgatttactggcagggaaaggagattccgggaaatgaaactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z