##gff-version 3
##sequence-region BBa_J23029 1 962
BBa_J23029	.	engineered_region	165	962	.	+	0	ID=BBa_F1610;Name=BBa_F1610
BBa_J23029	.	terminator	922	962	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F1610
BBa_J23029	.	polyA_site	949	962	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J23029	.	engineered_region	922	962	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J23029	.	stem_loop	929	948	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J23029	.	stop_codon	955	955	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J23029	.	CDS	183	800	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_F1610
BBa_J23029	.	CDS	183	761	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_J23029	.	engineered_region	183	800	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_J23029	.	tag	762	793	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_J23029	.	engineered_tag	801	825	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_J23029	.	terminator	834	913	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F1610
BBa_J23029	.	stem_loop	845	888	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J23029	.	engineered_region	834	913	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J23029	.	ribosome_entry_site	165	176	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F1610
BBa_J23029	.	conserved_region	169	172	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J23029	.	mature_transcript_region	1	156	.	+	0	ID=BBa_J01129;Name=BBa_J01129
BBa_J23029	.	mature_transcript_region	63	156	.	+	0	ID=BBa_J01086;Name=BBa_J01086;Parent=BBa_J01129
BBa_J23029	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J01129
BBa_J23029	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J23029	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J23029	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J23029	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J23029	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
>BBa_J23029
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagacccaaaagcaggaggt
gaatctagtaggtggttaatgaaaattaacttacttactagaaatatctctaaaaagccagattattaatccggcttta
ctagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtata
aaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatc
agatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgttta
ttacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatccta
atatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaa
actatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagag
cgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcgg
ttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagt
agcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttata