BBa_J23008
1
BBa_J23008
[key3c] riboregulator for lock3 variants
2006-07-09T11:00:00Z
2015-08-31T04:08:38Z
J23007
This part has the same homology region to J01122 as J23007 except that unlike J23007, J23008 does not contain the hairpin that was derived from J01129. J23008 anneals linearly to the stem of the hairpin in J01122 with perfect base pairing and destroys the hairpin in the process and reveals the RBS on J01122. When J01122 is transcribed the RNA secondary structure is such that the RBS hairpins with sequence upstream preventing the ribosome from binding and translation from occuring thereby locking up RFP further down stream. When J23008 is introduced into the cell in trans, we suspect that it will unlock the RBS for RFP as described above allowing translation to occur.
false
false
_52_
0
931
52
In stock
false
No design considerations
true
Kaitlin Davis
BBa_J23044
1
BBa_J23044
[key3d][key3c]
2006-08-03T11:00:00Z
2015-08-31T04:08:39Z
J23009.J23008
[key3d][key3c]
false
false
_52_
0
483
95
It's complicated
false
N/A
false
John Anderson
component1893450
1
BBa_J23009
component1893451
1
BBa_J23008
annotation1893450
1
BBa_J23009
range1893450
1
1
97
annotation1893451
1
BBa_J23008
range1893451
1
106
199
BBa_J23009
1
BBa_J23009
[key3d] riboregulator for lock3 variants
2006-08-02T11:00:00Z
2015-08-31T04:08:38Z
Overlap extension
Variant of key3 (see part J01129 (key3), J23007(key3b), J23008(key3c)). Part is in pJ23006, so there is a Ptet upstream of the XbaI site to drive expression. Nevertheless, J23009 is still a basic part allowing both prefix and suffix insertions.
false
false
_52_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_J23008_sequence
1
acccaaaagcaagaggtgattctagttggtggttaatgaaaattaacttacttactagaaatatctctaaaaagccagattattaatccggctt
BBa_J23009_sequence
1
cgggcgggcgggcgggcgggatccataaaaaaaaaacccaaaagcaagaggtgattctagttaaaaaaaaaaagcttcccgcccgcccgcccgcccg
BBa_J23044_sequence
1
cgggcgggcgggcgggcgggatccataaaaaaaaaacccaaaagcaagaggtgattctagttaaaaaaaaaaagcttcccgcccgcccgcccgcccgtactagagacccaaaagcaagaggtgattctagttggtggttaatgaaaattaacttacttactagaaatatctctaaaaagccagattattaatccggctt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z