LOCUS       BBa_J23047              2347 bp    DNA     linear       22-Nov-2024
DEFINITION  [lock3b][RFP]
ACCESSION   BBa_J23047
VERSION     BBa_J23047.1  
FEATURES             Location/Qualifiers
     misc_feature    1..41
                     /label=BBa_J23030
     misc_feature    2129..2257
                     /label=BBa_B0015 terminator
     misc_feature    2266..2319
                     /label=BBa_R0040 (Ptet)
     misc_feature    232..851
                     /label=pBR322 origin
     misc_feature    229..944
                     /label=pMB1 origin
     CDS             1006..1866
                     /label=beta-lactamase (ampicillin resistance marker)
     promoter        1908..1936
                     /label=beta-lactamase (ampR) promoter
     stem_loop       2140..2183
                     /label=rrnB T1 terminator
     misc_feature    50..70
                     /label=BioBrick suffix
     misc_feature    2107..2112
                     /label=EcoRI
     misc_feature    2113..2120
                     /label=NotI
     misc_feature    2341..2346
                     /label=XbaI
     primer_bind     1989..2008
                     /label=Verification forward (VF2) primer binding site
     primer_bind     128..147
                     /label=Verification reverse (VR) primer binding site
     misc_feature    2328..2339
                     /label=BBa_B0034
     RBS             2328..2339
                     /label=RBS
     promoter        2266..2319
                     /label=Ptet
     misc_feature    50..2347
                     /label=BBa_J23018
ORIGIN
        1 aactagaatc acctcttgct tttgggtaag acaagaggag atactagagt actagtagcg
       61 gccgctgcag gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
      121 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
      181 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
      241 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
      301 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
      361 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
      421 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
      481 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
      541 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
      601 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
      661 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
      721 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
      781 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
      841 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
      901 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
      961 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     1021 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     1081 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     1141 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     1201 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     1261 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     1321 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     1381 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     1441 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     1501 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     1561 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     1621 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     1681 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     1741 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     1801 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     1861 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     1921 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     1981 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     2041 taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga
     2101 tttctggaat tcgcggccgc aactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     2161 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     2221 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagtccct atcagtgata
     2281 gagattgaca tccctatcag tgatagagat actgagcact actagagaaa gaggagaaat
     2341 tctagag
//