LOCUS       BBa_J23048              2348 bp    DNA     linear       12-Mar-2025
DEFINITION  [lock3c][RFP]
ACCESSION   BBa_J23048
VERSION     BBa_J23048.1  
FEATURES             Location/Qualifiers
     misc_feature    1..42
                     /label=BBa_J23031
     misc_feature    2130..2258
                     /label=BBa_B0015 terminator
     misc_feature    2267..2320
                     /label=BBa_R0040 (Ptet)
     misc_feature    233..852
                     /label=pBR322 origin
     misc_feature    230..945
                     /label=pMB1 origin
     CDS             1007..1867
                     /label=beta-lactamase (ampicillin resistance marker)
     promoter        1909..1937
                     /label=beta-lactamase (ampR) promoter
     stem_loop       2141..2184
                     /label=rrnB T1 terminator
     misc_feature    51..71
                     /label=BioBrick suffix
     misc_feature    2108..2113
                     /label=EcoRI
     misc_feature    2114..2121
                     /label=NotI
     misc_feature    2342..2347
                     /label=XbaI
     primer_bind     1990..2009
                     /label=Verification forward (VF2) primer binding site
     primer_bind     129..148
                     /label=Verification reverse (VR) primer binding site
     misc_feature    2329..2340
                     /label=BBa_B0034
     RBS             2329..2340
                     /label=RBS
     promoter        2267..2320
                     /label=Ptet
     misc_feature    51..2348
                     /label=BBa_J23018
ORIGIN
        1 aactagaatc acctcttgct tttgggtaag acgaagagga gatactagag tactagtagc
       61 ggccgctgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      121 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
      181 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      241 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      301 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      361 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      421 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
      481 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
      541 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
      601 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
      661 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
      721 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
      781 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
      841 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
      901 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
      961 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     1021 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     1081 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     1141 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     1201 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     1261 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     1321 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     1381 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     1441 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     1501 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     1561 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     1621 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     1681 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     1741 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     1801 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     1861 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     1921 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     1981 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     2041 ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg
     2101 atttctggaa ttcgcggccg caactagagc caggcatcaa ataaaacgaa aggctcagtc
     2161 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     2221 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtccc tatcagtgat
     2281 agagattgac atccctatca gtgatagaga tactgagcac tactagagaa agaggagaaa
     2341 ttctagag
//