##gff-version 3
##sequence-region BBa_J23048 1 2348
BBa_J23048	.	mature_transcript_region	1	42	.	+	0	ID=BBa_J23031;Name=BBa_J23031
BBa_J23048	.	plasmid_vector	51	2348	.	+	0	ID=BBa_J23018;Name=BBa_J23018
BBa_J23048	.	sequence_feature	2108	2113	.	+	0	ID=annotation1975738;Name=EcoRI;Parent=BBa_J23018
BBa_J23048	.	ribosome_entry_site	2329	2340	.	+	0	ID=annotation1975764;Name=RBS;Parent=BBa_J23018
BBa_J23048	.	CDS	1007	1867	.	+	0	ID=annotation1975734;Name=beta-lactamase (ampicillin resistance marker);Parent=BBa_J23018
BBa_J23048	.	sequence_feature	233	852	.	+	0	ID=annotation1893437;Name=pBR322 origin;Parent=BBa_J23018
BBa_J23048	.	primer_binding_site	1990	2009	.	+	0	ID=annotation1975760;Name=Verification forward (VF2) primer binding site;Parent=BBa_J23018
BBa_J23048	.	sequence_feature	2342	2347	.	+	0	ID=annotation1975740;Name=XbaI;Parent=BBa_J23018
BBa_J23048	.	promoter	1909	1937	.	+	0	ID=annotation1975735;Name=beta-lactamase (ampR) promoter;Parent=BBa_J23018
BBa_J23048	.	stem_loop	2141	2184	.	+	0	ID=annotation1975736;Name=rrnB T1 terminator;Parent=BBa_J23018
BBa_J23048	.	sequence_feature	2114	2121	.	+	0	ID=annotation1975739;Name=NotI;Parent=BBa_J23018
BBa_J23048	.	engineered_region	2329	2340	.	+	0	ID=annotation1975763;Name=BBa_B0034;Parent=BBa_J23018
BBa_J23048	.	engineered_region	2130	2258	.	+	0	ID=annotation1893435;Name=BBa_B0015 terminator;Parent=BBa_J23018
BBa_J23048	.	engineered_region	2267	2320	.	+	0	ID=annotation1893436;Name=BBa_R0040 (Ptet);Parent=BBa_J23018
BBa_J23048	.	sequence_feature	230	945	.	+	0	ID=annotation1934517;Name=pMB1 origin;Parent=BBa_J23018
BBa_J23048	.	primer_binding_site	129	148	.	+	0	ID=annotation1975761;Name=Verification reverse (VR) primer binding site;Parent=BBa_J23018
BBa_J23048	.	sequence_feature	51	71	.	+	0	ID=annotation1975737;Name=BioBrick suffix;Parent=BBa_J23018
BBa_J23048	.	promoter	2267	2320	.	+	0	ID=annotation1975765;Name=Ptet;Parent=BBa_J23018
>BBa_J23048
aactagaatcacctcttgcttttgggtaagacgaagaggagatactagagtactagtagcggccgctgcaggcttcctc
gctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatcc
acagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgt
tgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaaccc
gacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttacc
ggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgt
aggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcg
tcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtat
gtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctc
tgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggttt
ttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgac
gctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaa
attaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtga
ggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgg
gagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataa
accagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccg
ggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgc
tcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaa
aagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagc
actgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctga
gaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaa
aagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgta
acccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaa
aatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagca
tttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcac
atttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacg
aggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcaactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgaca
tccctatcagtgatagagatactgagcactactagagaaagaggagaaattctagag