##gff-version 3
##sequence-region BBa_J23049 1 2349
BBa_J23049	.	mature_transcript_region	1	43	.	+	0	ID=BBa_J23032;Name=BBa_J23032
BBa_J23049	.	plasmid_vector	52	2349	.	+	0	ID=BBa_J23018;Name=BBa_J23018
BBa_J23049	.	sequence_feature	2109	2114	.	+	0	ID=annotation1975738;Name=EcoRI;Parent=BBa_J23018
BBa_J23049	.	sequence_feature	2343	2348	.	+	0	ID=annotation1975740;Name=XbaI;Parent=BBa_J23018
BBa_J23049	.	engineered_region	2131	2259	.	+	0	ID=annotation1893435;Name=BBa_B0015 terminator;Parent=BBa_J23018
BBa_J23049	.	sequence_feature	2115	2122	.	+	0	ID=annotation1975739;Name=NotI;Parent=BBa_J23018
BBa_J23049	.	primer_binding_site	130	149	.	+	0	ID=annotation1975761;Name=Verification reverse (VR) primer binding site;Parent=BBa_J23018
BBa_J23049	.	sequence_feature	231	946	.	+	0	ID=annotation1934517;Name=pMB1 origin;Parent=BBa_J23018
BBa_J23049	.	sequence_feature	234	853	.	+	0	ID=annotation1893437;Name=pBR322 origin;Parent=BBa_J23018
BBa_J23049	.	stem_loop	2142	2185	.	+	0	ID=annotation1975736;Name=rrnB T1 terminator;Parent=BBa_J23018
BBa_J23049	.	engineered_region	2330	2341	.	+	0	ID=annotation1975763;Name=BBa_B0034;Parent=BBa_J23018
BBa_J23049	.	promoter	1910	1938	.	+	0	ID=annotation1975735;Name=beta-lactamase (ampR) promoter;Parent=BBa_J23018
BBa_J23049	.	primer_binding_site	1991	2010	.	+	0	ID=annotation1975760;Name=Verification forward (VF2) primer binding site;Parent=BBa_J23018
BBa_J23049	.	engineered_region	2268	2321	.	+	0	ID=annotation1893436;Name=BBa_R0040 (Ptet);Parent=BBa_J23018
BBa_J23049	.	ribosome_entry_site	2330	2341	.	+	0	ID=annotation1975764;Name=RBS;Parent=BBa_J23018
BBa_J23049	.	CDS	1008	1868	.	+	0	ID=annotation1975734;Name=beta-lactamase (ampicillin resistance marker);Parent=BBa_J23018
BBa_J23049	.	promoter	2268	2321	.	+	0	ID=annotation1975765;Name=Ptet;Parent=BBa_J23018
BBa_J23049	.	sequence_feature	52	72	.	+	0	ID=annotation1975737;Name=BioBrick suffix;Parent=BBa_J23018
>BBa_J23049
aactagaatcacctcttgcttttgggtaagacagaagaggagatactagagtactagtagcggccgctgcaggcttcct
cgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatc
cacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcg
ttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacc
cgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttac
cggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtg
taggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatc
gtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggta
tgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgct
ctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtt
tttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctga
cgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatcctttta
aattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtg
aggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacg
ggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaata
aaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgcc
gggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacg
ctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaa
aaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcag
cactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctg
agaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaacttta
aaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgt
aacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggca
aaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagc
atttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgca
catttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcac
gaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcaactagagcca
ggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcta
ctagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgac
atccctatcagtgatagagatactgagcactactagagaaagaggagaaattctagag