##gff-version 3
##sequence-region BBa_J23051 1 2346
BBa_J23051	.	plasmid_vector	49	2346	.	+	0	ID=BBa_J23018;Name=BBa_J23018
BBa_J23051	.	sequence_feature	2106	2111	.	+	0	ID=annotation1975738;Name=EcoRI;Parent=BBa_J23018
BBa_J23051	.	sequence_feature	2112	2119	.	+	0	ID=annotation1975739;Name=NotI;Parent=BBa_J23018
BBa_J23051	.	sequence_feature	2340	2345	.	+	0	ID=annotation1975740;Name=XbaI;Parent=BBa_J23018
BBa_J23051	.	engineered_region	2327	2338	.	+	0	ID=annotation1975763;Name=BBa_B0034;Parent=BBa_J23018
BBa_J23051	.	sequence_feature	231	850	.	+	0	ID=annotation1893437;Name=pBR322 origin;Parent=BBa_J23018
BBa_J23051	.	CDS	1005	1865	.	+	0	ID=annotation1975734;Name=beta-lactamase (ampicillin resistance marker);Parent=BBa_J23018
BBa_J23051	.	stem_loop	2139	2182	.	+	0	ID=annotation1975736;Name=rrnB T1 terminator;Parent=BBa_J23018
BBa_J23051	.	ribosome_entry_site	2327	2338	.	+	0	ID=annotation1975764;Name=RBS;Parent=BBa_J23018
BBa_J23051	.	promoter	2265	2318	.	+	0	ID=annotation1975765;Name=Ptet;Parent=BBa_J23018
BBa_J23051	.	primer_binding_site	127	146	.	+	0	ID=annotation1975761;Name=Verification reverse (VR) primer binding site;Parent=BBa_J23018
BBa_J23051	.	engineered_region	2128	2256	.	+	0	ID=annotation1893435;Name=BBa_B0015 terminator;Parent=BBa_J23018
BBa_J23051	.	sequence_feature	228	943	.	+	0	ID=annotation1934517;Name=pMB1 origin;Parent=BBa_J23018
BBa_J23051	.	promoter	1907	1935	.	+	0	ID=annotation1975735;Name=beta-lactamase (ampR) promoter;Parent=BBa_J23018
BBa_J23051	.	primer_binding_site	1988	2007	.	+	0	ID=annotation1975760;Name=Verification forward (VF2) primer binding site;Parent=BBa_J23018
BBa_J23051	.	sequence_feature	49	69	.	+	0	ID=annotation1975737;Name=BioBrick suffix;Parent=BBa_J23018
BBa_J23051	.	engineered_region	2265	2318	.	+	0	ID=annotation1893436;Name=BBa_R0040 (Ptet);Parent=BBa_J23018
BBa_J23051	.	mature_transcript_region	1	40	.	+	0	ID=BBa_J23034;Name=BBa_J23034
>BBa_J23051
aactagaatcaattcttgcttttgggtaagaaagaggagatactagagtactagtagcggccgctgcaggcttcctcgc
tcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccac
agaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttg
ctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccga
caggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccgg
atacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtag
gtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtc
ttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgt
aggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctg
ctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggttttt
ttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgc
tcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaat
taaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgagg
cacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacggga
gggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaac
cagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccggg
aagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctc
gtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaa
gcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcac
tgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgaga
atagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaa
gtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaac
ccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaa
tgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatt
tatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacat
ttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgag
gcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcaactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatc
cctatcagtgatagagatactgagcactactagagaaagaggagaaattctagag