BBa_J23058 1 BBa_J23058 [GFPmut3] inserted into [key3d] 2006-08-08T11:00:00Z 2015-08-31T04:08:39Z J23009 and pG80-GFPmut3 This is an intermediate that aids in cloning via introducing a phenotype that allows for easy distinction between the parent and clone. The goal is build a construct similiar to J23009 with a longer hairpin loop (10 bases total). By inserting this in the BamHI and HindIII sites the successful subclones should be green (hence GFP), parent will be white. Conversely, when cutting out the GFPmut3 cassette and inserting the longer hairpin the successful clones will be white and the parent will still be green. This makes screening colonies much easier. false false _52_ 0 936 52 Not in stock false none false bryan hernandez annotation1895138 1 GFPmut3 range1895138 1 42 758 BBa_J23058_sequence 1 cgggcgggcgggcgggcgggatccataaggaggaaaaacatatgagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttgacttatggtgttcaatgcttttcaagatacccagatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataagaattcgaagcttcccgcccgcccgcccgcccg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z