BBa_J23069 1 BBa_J23069 TraGr KO 2006-08-13T11:00:00Z 2015-08-31T04:08:39Z <tt> PCR JL20/JL21 on RP4 gen. (2268 bp, MfeI/BglII)<br> Sub into pGLN (BamHI/EcoRI)<br> Product is pJL20(J23067)<br> ------------------------------------------------ IPCR JL22/JL23 on pJL20(J23067) (3454 bp, EcoRI/PstI)<br> Sub in pSB1A2-Bca9007 (1015 bp, EcoRI/PstI)<br> Product is pJL22(J23068)<br> ------------------------------------------------ PCR JL18/JL19 on pJL22(J23068) for D/W (bp, DpnI)<br> Datsenko Wanner on pKD46/Rlambda,<br> Product is J23069<br> ------------------------------------------------ JL20 Forward BglII to BamHI for traG-5' knockout cassette<br> GAGTCAGATCTgattatgttggtgcgctg<br> JL21 Reverse MfeI to EcoRI for traG-5' knockout cassette<br> gctggCAATTGcattcgatttccagtacaggc<br> JL22 Reverse EcoRI IPCR of traG<br> gctgcGAATTCgatactggaaggaatgggc<br> JL23 Forward PstI IPCR of traG<br> cattgCTGCAGacggcagcattgaagaggcg J23068 Datsenko Wanner on pKD46/Rlambda false false _52_ 0 483 95 Not in stock false n/a false John Anderson BBa_J23069_sequence 1 ctgattatgttggtgcgctgtcctatgcaggcacccggaacgtcgaggaccaggcgctggccctgttgaggaaagaaaacgacgagattctggtgctgcccgtcgataaggccacggtgcaacgcatgaagcgcctcgccatcggtgatcccgtcaccgtgaccccgcgaggctcccttaaaactacccgaggtaggagtagatgaagaaccgaaacaacgccgtggggccacagatacgggcgaaaaaaccgaaggccagcaagaccgttcccatcctcgccggcctgtcccttggggcaggcttgcagaccgcgacgcaatatttcgcccattccttccagtatcgaattcgcggccgcttctagaggaagttcctatactttttagagaataggaacttctactagaggaataggaacttcatttaaatggcgcgccttacgccccgccctgccactcatcgcagtactgttgtattcattaagcatctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgtaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgacaactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgccgatcaacgtctcattttcgccaaaagttggcccagggcttcccggtatcaacagggacaccaggatttatttattctgcgaagtgatcttccgtcacaggtaggcgcgccactagaggaagttcctatactttttagagaataggaacttctactagtagcggccgctgcagacggcagcattgaagaggcgggcgacatggttgtgtacgtcgccggctatcccgcgatctacggcaagcagccgctctacttcaaagacccgatattccaggcccgcgcggccgtaccggctccgaaggtcagcgacaagctgatccagacggccaccgtcgaggaaggggaggggatcacgatatgagccgcttccagcgcctcaccaagtacgtcgccatcggaggcggcgcggccttgttgctcgccggcgcggcctatctcgccggcgcgaaggtcaacaccaccaaaagcattccggtcggcctgtactggaaatcgaatg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z