BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_J23104 1 BBa_J23104 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z isolated from library of promoters replace later false false _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_J23013 1 [TraGr] TraGr open reading frame basic part 2006-07-23T11:00:00Z 2015-08-31T04:08:39Z PCR JL10/JL11 on Rlambda (?bp, EcoRI/SpeI, Neb2) Paste into pSB1A2-I13521 (EcoRI/SpeI, Neb2, Large 2062) TraGr complement false false _52_ 0 363 52 Not in stock false JL10 Forward EcoRI oligo to bioBrick TraG: CCTAGGAATTCGCGGCCGCTTCTAGatgaagaaccgaaacaacgc JL11 Reverse SpeI oligo to bioBrick TraG: gacttACTAGTgaagcggctcatatcgtgatc false Will Bosworth BBa_J23074 1 BBa_J23074 [RA2 Promoter][RBS][TraGr][TT] 2006-08-24T11:00:00Z 2015-08-31T04:08:39Z [RA2 Promoter][RBS][TraGr][TT]<br> Digest PCR product of pSB1A2-J23038 with G00101 and ca641R (BSAI/SpeI, Neb2, ~1600 )<br> Paste into pSB1AK3-J23026 (XbaI/BsaI, Neb2, Large 5229)<br> Product is pSB1AK3-J23074. [RA2 Promoter][RBS][TraGr][TT] false false _52_ 0 933 52 Not in stock false pSB1A2-J23038 has a really short sequence between XbaI and SpeI so okay to PCR because miniprep was messy. false Samantha Liang component1900901 1 BBa_J23104 component1900905 1 BBa_B0010 component1900903 1 BBa_B0034 component1900907 1 BBa_B0012 component1900904 1 BBa_J23013 annotation1900901 1 BBa_J23104 range1900901 1 1 35 annotation1900907 1 BBa_B0012 range1900907 1 2073 2113 annotation1900903 1 BBa_B0034 range1900903 1 44 55 annotation1900904 1 BBa_J23013 range1900904 1 62 1976 annotation1900905 1 BBa_B0010 range1900905 1 1985 2064 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_J23074_sequence 1 ttgacagctagctcagtcctaggtattgtgctagctactagagaaagaggagaaatactagatgaagaaccgaaacaacgccgtggggccacagatacgggcgaaaaaaccgaaggccagcaagaccgttcccatcctcgccggcctgtcccttggggcaggcttgcagaccgcgacgcaatatttcgcccattccttccagtatcaggccggacttggctggaatatcaaccacgtctacacgccctggtcgatccttcaatgggccggcaagtggtacggccaatatcccgacgatttcatgcgcgcggccagcatgggtatggtcgtttcgaccgtgggcctgctgggcacggccgtcacgcagatggtcaaggcgaacaccggcaaggcgaacgactatctgcacggttcggcccgctgggccgacaagaaggacatacaggccgccggcctgctgccccggccgcgcaccgtcgtcgagctggtgtcaggcaaacacccgcccacttccagcggcgtctatgtgggcggctggcaggacaaggacggcaagttccactacctgcggcacaacggccccgagcacgtcttgacctacgcgccgacgcgctccggcaagggcgtcggcctggtcgttccgacgctgctttcctgggcgcacagcgccgtcatcaccgacctgaaaggcgagttgtgggcgctgaccgccggctggcggaagaagcatgcccgcaacaaggtcgtgcgctttgagccggcatccgcgcagggtagcgcgtgctggaacccgctcgatgaaatccgcttggggaccgagtacgaggttggcgacgtgcagaaccttgccaccctgatcgtcgatccggacggcaagggcctggaatcgcactggcagaaaaccagccaggcgctgcttgtcggcgtcatcctgcacgcgctctacaaggctaagaacgagggtacgccggccaccctgccgtcggtggacggcatgcttgccgacccgaaccgcgacgtgggcgagctttggatggaaatgaccacctacggccacgttgacgggcagaaccaccctgcggtcggctctgcggcccgcgacatgatggaccgcccggaggaagaatccggttccgtgctgtccaccgccaagtcctacctggccctgtaccgcgacccggttgtggcccgcaacgtcagcaagtccgacttccgcatcaagcaactgatgcaccacgacgacccggtaagcctgttcatcgtgacgcagcccaacgacaaggcccgtctgcggcctctggtgcgcgtcatggtcaacatgatcgtccggctgctggccgacaagatggacttcgagaacggccgtcctgtcgcgcactacaagcatcgtctgctgatgatgctcgacgagttccccagcctgggcaagctcgaaatcctgcaagagtcccttgccttcgtcgccggctacggcatcaagtgctacctcatctgccaggacattaaccagctcaagagccgcgaaaccggctacggccacgacgaaagcatcacgtcgaactgccacgtgcagaacgcctacccgccgaaccgcgtggagacggccgagcacctgtcaaaactgacaggcaccacgaccatcgtgaaggagcagatcacgacgagcggccgccgcacgtcggcgctgttgggcaacgtctcgcggaccttccaggaagtgcagcggccattgctgacccccgatgaatgcctgcgcatgccggggccgaagaagagcgccgacggcagcattgaagaggcgggcgacatggttgtgtacgtcgccggctatcccgcgatctacggcaagcagccgctctacttcaaagacccgatattccaggcccgcgcggccgtaccggctccgaaggtcagcgacaagctgatccagacggccaccgtcgaggaaggggaggggatcacgatatgagccgctttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J23104_sequence 1 ttgacagctagctcagtcctaggtattgtgctagc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J23013_sequence 1 atgaagaaccgaaacaacgccgtggggccacagatacgggcgaaaaaaccgaaggccagcaagaccgttcccatcctcgccggcctgtcccttggggcaggcttgcagaccgcgacgcaatatttcgcccattccttccagtatcaggccggacttggctggaatatcaaccacgtctacacgccctggtcgatccttcaatgggccggcaagtggtacggccaatatcccgacgatttcatgcgcgcggccagcatgggtatggtcgtttcgaccgtgggcctgctgggcacggccgtcacgcagatggtcaaggcgaacaccggcaaggcgaacgactatctgcacggttcggcccgctgggccgacaagaaggacatacaggccgccggcctgctgccccggccgcgcaccgtcgtcgagctggtgtcaggcaaacacccgcccacttccagcggcgtctatgtgggcggctggcaggacaaggacggcaagttccactacctgcggcacaacggccccgagcacgtcttgacctacgcgccgacgcgctccggcaagggcgtcggcctggtcgttccgacgctgctttcctgggcgcacagcgccgtcatcaccgacctgaaaggcgagttgtgggcgctgaccgccggctggcggaagaagcatgcccgcaacaaggtcgtgcgctttgagccggcatccgcgcagggtagcgcgtgctggaacccgctcgatgaaatccgcttggggaccgagtacgaggttggcgacgtgcagaaccttgccaccctgatcgtcgatccggacggcaagggcctggaatcgcactggcagaaaaccagccaggcgctgcttgtcggcgtcatcctgcacgcgctctacaaggctaagaacgagggtacgccggccaccctgccgtcggtggacggcatgcttgccgacccgaaccgcgacgtgggcgagctttggatggaaatgaccacctacggccacgttgacgggcagaaccaccctgcggtcggctctgcggcccgcgacatgatggaccgcccggaggaagaatccggttccgtgctgtccaccgccaagtcctacctggccctgtaccgcgacccggttgtggcccgcaacgtcagcaagtccgacttccgcatcaagcaactgatgcaccacgacgacccggtaagcctgttcatcgtgacgcagcccaacgacaaggcccgtctgcggcctctggtgcgcgtcatggtcaacatgatcgtccggctgctggccgacaagatggacttcgagaacggccgtcctgtcgcgcactacaagcatcgtctgctgatgatgctcgacgagttccccagcctgggcaagctcgaaatcctgcaagagtcccttgccttcgtcgccggctacggcatcaagtgctacctcatctgccaggacattaaccagctcaagagccgcgaaaccggctacggccacgacgaaagcatcacgtcgaactgccacgtgcagaacgcctacccgccgaaccgcgtggagacggccgagcacctgtcaaaactgacaggcaccacgaccatcgtgaaggagcagatcacgacgagcggccgccgcacgtcggcgctgttgggcaacgtctcgcggaccttccaggaagtgcagcggccattgctgacccccgatgaatgcctgcgcatgccggggccgaagaagagcgccgacggcagcattgaagaggcgggcgacatggttgtgtacgtcgccggctatcccgcgatctacggcaagcagccgctctacttcaaagacccgatattccaggcccgcgcggccgtaccggctccgaaggtcagcgacaagctgatccagacggccaccgtcgaggaaggggaggggatcacgatatgagccgctt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z