##gff-version 3
##sequence-region BBa_J23079 1 921
BBa_J23079	.	CDS	79	784	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_J23079	.	engineered_tag	760	784	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J23079	.	CDS	79	753	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J23079	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J23079	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J23079	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J23079	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J23079	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J23079	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J23079	.	terminator	793	921	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J23079	.	terminator	881	921	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J23079	.	polyA_site	908	921	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J23079	.	stem_loop	888	907	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J23079	.	engineered_region	881	921	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J23079	.	stop_codon	914	914	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J23079	.	terminator	793	872	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J23079	.	stem_loop	804	847	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J23079	.	engineered_region	793	872	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J23079	.	ribosome_entry_site	63	72	.	+	0	ID=BBa_J23080;Name=BBa_J23080
>BBa_J23079
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagatactaga
tggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagtt
cgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccg
ctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcc
cggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttac
cgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgac
ggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaag
gtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaa
accggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgtt
gaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactag
agccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct
ctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata