LOCUS       BBa_J23120              1342 bp    DNA     linear       13-May-2024
DEFINITION  Locked RFP (J23077) with OriT (J01003). Lock that can be 
            transferred via conjugation
ACCESSION   BBa_J23120
VERSION     BBa_J23120.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    63..114
                     /label=BBa_J23078
     CDS             121..795
                     /label=mrfp1
     misc_feature    802..826
                     /label=Help:Barcodes
     CDS             121..826
                     /label=BBa_E1010
     stem_loop       846..889
                     /label=stem_loop
     misc_feature    835..914
                     /label=BBa_B0010
     terminator      835..914
                     /label=BBa_B0010
     stem_loop       930..949
                     /label=T7 TE
     misc_feature    956..956
                     /label=stop
     polyA_site      950..963
                     /label=polya
     misc_feature    923..963
                     /label=BBa_B0012
     terminator      923..963
                     /label=BBa_B0012
     terminator      835..963
                     /label=BBa_B0015
     misc_feature    1..963
                     /label=BBa_J23077
     oriT            972..1342
                     /label=BBa_J01003
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtgtagacc gaactagaat cacctcttgc ttttgggtaa gacagaagag gagatactag
      121 atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt
      181 tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt
      241 acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc
      301 ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg
      361 gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa
      421 gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac
      481 aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc
      541 atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa
      601 atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc
      661 tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac
      721 atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt
      781 cactccaccg gtgcttaata acgctgatag tgctagtgta gatcgctact agagccaggc
      841 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      901 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      961 atatactaga ggccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct
     1021 tgataggtgg gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat
     1081 ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca
     1141 ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta
     1201 tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa
     1261 atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga
     1321 agcagggtta tgcagcggaa aa
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