##gff-version 3
##sequence-region BBa_J23120 1 1342
BBa_J23120	.	oriT	972	1342	.	+	0	ID=BBa_J01003;Name=BBa_J01003
BBa_J23120	.	engineered_region	1	963	.	+	0	ID=BBa_J23077;Name=BBa_J23077
BBa_J23120	.	CDS	121	826	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J23077
BBa_J23120	.	CDS	121	795	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J23120	.	engineered_tag	802	826	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J23120	.	terminator	835	963	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_J23077
BBa_J23120	.	terminator	835	914	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J23120	.	stem_loop	846	889	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J23120	.	engineered_region	835	914	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J23120	.	terminator	923	963	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J23120	.	stop_codon	956	956	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J23120	.	polyA_site	950	963	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J23120	.	stem_loop	930	949	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J23120	.	engineered_region	923	963	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J23120	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J23077
BBa_J23120	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J23120	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J23120	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J23120	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J23120	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J23120	.	mature_transcript_region	63	114	.	+	0	ID=BBa_J23078;Name=BBa_J23078;Parent=BBa_J23077
>BBa_J23120
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtgtagaccgaactagaa
tcacctcttgcttttgggtaagacagaagaggagatactagatggcttcctccgaagacgttatcaaagagttcatgcg
tttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaa
ggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttcc
agtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaa
atgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttc
atctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaag
cttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtgg
tcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatc
aaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccg
gtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaa
gactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggc
ctttctgcgtttatatactagaggccgccttttcctcaatcgctcttcgttcgtctggaaggcagtacaccttgatagg
tgggctgcccttcctggttggcttggtttcatcagccatccgcttgccctcatctgttacgccggcggtagccggccag
cctcgcagagcaggattcccgttgagcaccgccaggtgcgaataagggacagtgaagaaggaacacccgctcgcgggtg
ggcctacttcacctatcctgcccggctgacgccgttggatacaccaaggaaagtctacacgaaccctttggcaaaatcc
tgtatatcgtgcgaaaaaggatggatataccgaaaaaatcgctataatgaccccgaagcagggttatgcagcggaaaa