LOCUS       BBa_J24006              1602 bp    DNA     linear       23-May-2024
ACCESSION   BBa_J24006
VERSION     BBa_J24006.1  
FEATURES             Location/Qualifiers
     misc_feature    580..611
                     /label=LVA
     CDS             1..579
                     /label=luxI
     misc_feature    1..618
                     /label=BBa_C0061
     misc_feature    619..643
                     /label=Help:Barcodes
     CDS             1..618
                     /label=BBa_C0061
     misc_feature    652..670
                     /label=TetR 1
     misc_feature    652..705
                     /label=BBa_R0040
     promoter        671..676
                     /label=-35
     misc_feature    677..695
                     /label=TetR 2
     promoter        694..699
                     /label=-10
     promoter        652..705
                     /label=BBa_R0040
     misc_feature    718..721
                     /label=conserved
     RBS             714..725
                     /label=BBa_B0034
     promoter        652..725
                     /label=BBa_J13002
     misc_feature    738..741
                     /label=conserved
     RBS             734..745
                     /label=BBa_B0034
     CDS             752..1462
                     /label=mCherry
     misc_feature    1103..1103
                     /label=C->T (removing PstI site)
     misc_feature    752..1465
                     /label=BBa_J06504
     stem_loop       1485..1528
                     /label=stem_loop
     misc_feature    1474..1553
                     /label=BBa_B0010
     terminator      1474..1553
                     /label=BBa_B0010
     stem_loop       1569..1588
                     /label=T7 TE
     misc_feature    1595..1595
                     /label=stop
     polyA_site      1589..1602
                     /label=polya
     misc_feature    1562..1602
                     /label=BBa_B0012
     terminator      1562..1602
                     /label=BBa_B0012
     misc_feature    734..1602
                     /label=BBa_J06702
ORIGIN
        1 atgactataa tgataaaaaa atcggatttt ttggcaattc catcggagga gtataaaggt
       61 attctaagtc ttcgttatca agtgtttaag caaagacttg agtgggactt agttgtagaa
      121 aataaccttg aatcagatga gtatgataac tcaaatgcag aatatattta tgcttgtgat
      181 gatactgaaa atgtaagtgg atgctggcgt ttattaccta caacaggtga ttatatgctg
      241 aaaagtgttt ttcctgaatt gcttggtcaa cagagtgctc ccaaagatcc taatatagtc
      301 gaattaagtc gttttgctgt aggtaaaaat agctcaaaga taaataactc tgctagtgaa
      361 attacaatga aactatttga agctatatat aaacacgctg ttagtcaagg tattacagaa
      421 tatgtaacag taacatcaac agcaatagag cgatttttaa agcgtattaa agttccttgt
      481 catcgtattg gagacaaaga aattcatgta ttaggtgata ctaaatcggt tgtattgtct
      541 atgcctatta atgaacagtt taaaaaagca gtcttaaatg ctgcaaacga cgaaaactac
      601 gctttagtag cttaataact ctgatagtgc tagtgtagat ctctactaga gtccctatca
      661 gtgatagaga ttgacatccc tatcagtgat agagatactg agcactacta gagaaagagg
      721 agaaatacta gagaaagagg agaaatacta gatggtgagc aagggcgagg aggataacat
      781 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
      841 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
      901 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
      961 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     1021 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     1081 gaccgtgacc caggactcct ccttgcagga cggcgagttc atctacaagg tgaagctgcg
     1141 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     1201 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     1261 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     1321 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     1381 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     1441 catggacgag ctgtacaagt aataatacta gagccaggca tcaaataaaa cgaaaggctc
     1501 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     1561 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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