##gff-version 3
##sequence-region BBa_J24006 1 1602
BBa_J24006	.	engineered_region	734	1602	.	+	0	ID=BBa_J06702;Name=BBa_J06702
BBa_J24006	.	terminator	1474	1553	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J06702
BBa_J24006	.	stem_loop	1485	1528	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24006	.	engineered_region	1474	1553	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J24006	.	engineered_region	752	1465	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_J06702
BBa_J24006	.	sequence_alteration	1103	1103	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_J24006	.	CDS	752	1462	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_J24006	.	ribosome_entry_site	734	745	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J06702
BBa_J24006	.	conserved_region	738	741	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24006	.	terminator	1562	1602	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J06702
BBa_J24006	.	polyA_site	1589	1602	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24006	.	engineered_region	1562	1602	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24006	.	stem_loop	1569	1588	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J24006	.	stop_codon	1595	1595	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J24006	.	promoter	652	725	.	+	0	ID=BBa_J13002;Name=BBa_J13002
BBa_J24006	.	ribosome_entry_site	714	725	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J13002
BBa_J24006	.	conserved_region	718	721	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24006	.	promoter	652	705	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J13002
BBa_J24006	.	engineered_region	652	705	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J24006	.	non_covalent_binding_site	652	670	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J24006	.	non_covalent_binding_site	677	695	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J24006	.	promoter	694	699	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J24006	.	promoter	671	676	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J24006	.	CDS	1	618	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_J24006	.	engineered_region	1	618	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_J24006	.	tag	580	611	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_J24006	.	engineered_tag	619	643	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_J24006	.	CDS	1	579	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
>BBa_J24006
atgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatc
aagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgc
agaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatg
ctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttg
ctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaaca
cgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagtt
ccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatg
aacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgcta
gtgtagatctctactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactag
agaaagaggagaaatactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatc
aaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagg
gccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcct
gtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttc
cccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgc
aggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagac
catgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaag
ctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcct
acaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgaggg
ccgccactccaccggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctca
gtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttata