LOCUS       BBa_J24008              1845 bp    DNA     linear       20-Apr-2024
DEFINITION  pLasR promoter with YFP and cI production
ACCESSION   BBa_J24008
VERSION     BBa_J24008.1  
FEATURES             Location/Qualifiers
     misc_feature    46..63
                     /label=OP2
     misc_feature    106..125
                     /label=OP1
     promoter        117..122
                     /label=-35
     promoter        140..145
                     /label=-10
     promoter        1..157
                     /label=BBa_R0079
     RBS             166..178
                     /label=RBS-3\Weak
     RBS             172..175
                     /label=RBS
     misc_feature    166..178
                     /label=BBa_B0032
     RBS             166..178
                     /label=BBa_B0032
     CDS             185..907
                     /label=BBa_E0030
     misc_feature    166..907
                     /label=BBa_I15017
     misc_feature    920..923
                     /label=conserved
     RBS             916..927
                     /label=BBa_B0034
     CDS             937..1644
                     /label=cI lambda
     misc_feature    1645..1677
                     /label=LVA
     misc_feature    1684..1708
                     /label=Help:Barcodes
     CDS             934..1683
                     /label=BBa_C0051
     stem_loop       1728..1771
                     /label=stem_loop
     misc_feature    1717..1796
                     /label=BBa_B0010
     terminator      1717..1796
                     /label=BBa_B0010
     stem_loop       1812..1831
                     /label=T7 TE
     misc_feature    1838..1838
                     /label=stop
     polyA_site      1832..1845
                     /label=polya
     misc_feature    1805..1845
                     /label=BBa_B0012
     terminator      1805..1845
                     /label=BBa_B0012
     misc_feature    916..1845
                     /label=BBa_P0451
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtttggc cgcgggttct ttttggtaca cgaaagctac tagagtcaca caggaaagta
      181 ctagatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      241 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      301 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      361 caccctcgtg accaccttcg gctacggcct gcaatgcttc gcccgctacc ccgaccacat
      421 gaagctgcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      481 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
      541 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
      601 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
      661 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
      721 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
      781 ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
      841 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
      901 gtaataatac tagagaaaga ggagaaatac tagatgagca caaaaaagaa accattaaca
      961 caagagcagc ttgaggacgc acgtcgcctt aaagcaattt atgaaaaaaa gaaaaatgaa
     1021 cttggcttat cccaggaatc tgtcgcagac aagatgggga tggggcagtc aggcgttggt
     1081 gctttattta atggcatcaa tgcattaaat gcttataacg ccgcattgct tgcaaaaatt
     1141 ctcaaagtta gcgttgaaga atttagccct tcaatcgcca gagaaatcta cgagatgtat
     1201 gaagcggtta gtatgcagcc gtcacttaga agtgagtatg agtaccctgt tttttctcat
     1261 gttcaggcag ggatgttctc acctgagctt agaaccttta ccaaaggtga tgcggagaga
     1321 tgggtaagca caaccaaaaa agccagtgat tctgcattct ggcttgaggt tgaaggtaat
     1381 tccatgaccg caccaacagg ctccaagcca agctttcctg acggaatgtt aattctcgtt
     1441 gaccctgagc aggctgttga gccaggtgat ttctgcatag ccagacttgg gggtgatgag
     1501 tttaccttca agaaactgat cagggatagc ggtcaggtgt ttttacaacc actaaaccca
     1561 cagtacccaa tgatcccatg caatgagagt tgttccgttg tggggaaagt tatcgctagt
     1621 cagtggcctg aagagacgtt tggcgctgca aacgacgaaa actacgcttt agtagcttaa
     1681 taacgctgat agtgctagtg tagatcgcta ctagagccag gcatcaaata aaacgaaagg
     1741 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1801 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//