LOCUS BBa_J24008 1845 bp DNA linear 22-Dec-2024 DEFINITION pLasR promoter with YFP and cI production ACCESSION BBa_J24008 VERSION BBa_J24008.1 FEATURES Location/Qualifiers misc_feature 46..63 /label=OP2 misc_feature 106..125 /label=OP1 promoter 117..122 /label=-35 promoter 140..145 /label=-10 promoter 1..157 /label=BBa_R0079 RBS 166..178 /label=RBS-3\Weak RBS 172..175 /label=RBS misc_feature 166..178 /label=BBa_B0032 RBS 166..178 /label=BBa_B0032 CDS 185..907 /label=BBa_E0030 misc_feature 166..907 /label=BBa_I15017 misc_feature 920..923 /label=conserved RBS 916..927 /label=BBa_B0034 CDS 937..1644 /label=cI lambda misc_feature 1645..1677 /label=LVA misc_feature 1684..1708 /label=Help:Barcodes CDS 934..1683 /label=BBa_C0051 stem_loop 1728..1771 /label=stem_loop misc_feature 1717..1796 /label=BBa_B0010 terminator 1717..1796 /label=BBa_B0010 stem_loop 1812..1831 /label=T7 TE misc_feature 1838..1838 /label=stop polyA_site 1832..1845 /label=polya misc_feature 1805..1845 /label=BBa_B0012 terminator 1805..1845 /label=BBa_B0012 misc_feature 916..1845 /label=BBa_P0451 ORIGIN 1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct 61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg 121 caggtttggc cgcgggttct ttttggtaca cgaaagctac tagagtcaca caggaaagta 181 ctagatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct 241 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac 301 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc 361 caccctcgtg accaccttcg gctacggcct gcaatgcttc gcccgctacc ccgaccacat 421 gaagctgcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat 481 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac 541 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg 601 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa 661 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct 721 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa 781 ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat 841 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa 901 gtaataatac tagagaaaga ggagaaatac tagatgagca caaaaaagaa accattaaca 961 caagagcagc ttgaggacgc acgtcgcctt aaagcaattt atgaaaaaaa gaaaaatgaa 1021 cttggcttat cccaggaatc tgtcgcagac aagatgggga tggggcagtc aggcgttggt 1081 gctttattta atggcatcaa tgcattaaat gcttataacg ccgcattgct tgcaaaaatt 1141 ctcaaagtta gcgttgaaga atttagccct tcaatcgcca gagaaatcta cgagatgtat 1201 gaagcggtta gtatgcagcc gtcacttaga agtgagtatg agtaccctgt tttttctcat 1261 gttcaggcag ggatgttctc acctgagctt agaaccttta ccaaaggtga tgcggagaga 1321 tgggtaagca caaccaaaaa agccagtgat tctgcattct ggcttgaggt tgaaggtaat 1381 tccatgaccg caccaacagg ctccaagcca agctttcctg acggaatgtt aattctcgtt 1441 gaccctgagc aggctgttga gccaggtgat ttctgcatag ccagacttgg gggtgatgag 1501 tttaccttca agaaactgat cagggatagc ggtcaggtgt ttttacaacc actaaaccca 1561 cagtacccaa tgatcccatg caatgagagt tgttccgttg tggggaaagt tatcgctagt 1621 cagtggcctg aagagacgtt tggcgctgca aacgacgaaa actacgcttt agtagcttaa 1681 taacgctgat agtgctagtg tagatcgcta ctagagccag gcatcaaata aaacgaaagg 1741 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 1801 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata //