<?xml version="1.0" ?>
<rdf:RDF xmlns:synbiohub="http://synbiohub.org#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#dateTime/" xmlns:rdfs="http://www.w3.org/2000/01/rdf-schema#" xmlns:sybio="http://www.sybio.ncl.ac.uk#" xmlns:ncbi="http://www.ncbi.nlm.nih.gov#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:igem="http://wiki.synbiohub.org/wiki/Terms/igem#" xmlns:sbh="http://wiki.synbiohub.org/wiki/Terms/synbiohub#" xmlns:sbol="http://sbols.org/v2#" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:gbconv="http://sbols.org/genBankConversion#" xmlns:genbank="http://www.ncbi.nlm.nih.gov/genbank#" xmlns:prov="http://www.w3.org/ns/prov#" xmlns:obo="http://purl.obolibrary.org/obo/" xmlns:om="http://www.ontology-of-units-of-measure.org/resource/om-2/" xmlns:dc="http://purl.org/dc/elements/1.1/">
  <sbol:ComponentDefinition rdf:about="https://synbiohub.org/public/igem/BBa_J24009/1">
    <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_J24009"/>
    <sbol:displayId>BBa_J24009</sbol:displayId>
    <sbol:version>1</sbol:version>
    <prov:wasDerivedFrom rdf:resource="http://parts.igem.org/Part:BBa_J24009"/>
    <prov:wasGeneratedBy rdf:resource="https://synbiohub.org/public/igem/igem2sbol/1"/>
    <dcterms:title>BBa_J24009</dcterms:title>
    <dcterms:description>Toggle Switch to Immobilize E. Coli</dcterms:description>
    <dcterms:created>2006-06-29T11:00:00Z</dcterms:created>
    <dcterms:modified>2015-08-31T04:08:41Z</dcterms:modified>
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acgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcgg
ttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgg
gaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgc
agaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaa
aatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtt
tccgctgctaacgacgaaaactacgctctggttgcttaataaccgaggaagcatgaagaatcaagcgcatccgattattgtcgtcaaacgacgcaaagccaaaagccacggggcagcacatggatcgtggaagattgcttat
gccgactttatgactgcgatgatggccttttttctggtgatgtggctgatctccatctccagcccaaaagagctgattcagattgcggagtacttccgga
ctccactggcgactgcggttacgggcggcgatcgcatttctaatagtgaaagcccaattcccggcggtggtgatgattacacccaaagccagggggaagt
gaataagcagccgaacatcgaagagctgaaaaaacgcatggagcaaagtcgattgcggaaattgcggggtgatctcgaccagttgatagagtccgatccg
aaactgcgggcgttacgtccccatctcaaaatcgatctggtccaggaaggtctacgtattcagatcatcgatagccagaatcgcccgatgtttagaaccg
gcagtgccgatgtcgaaccctatatgcgcgacattctgcgcgccattgcgcctgtactgaacggtattcccaaccgtattagcctttcaggtcataccga
tgatttcccctacgccagcggtgagaaaggatatagcaactgggagctttctgccgatcgggccaatgcatcccgccgcgaactgatggtcggagggttg
gatagcggcaaagtgttacgtgtcgtcggcatggcggcaacgatgcgcttaagcgatcgcggacctgatgatgccgtcaaccgtcgcatcagcctgctgg
tactgaacaaacaagccgaacaggccattttgcatgaaaacgccgaaagccagaatgagccagtaagcgccctggaaaaacctgaggttgcaccacaggt
cagtgttcccacaatgccatcagccgaaccgaggtgatcacacaggaaacctactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaa
aaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgct
tataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagta
tgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgc
ggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagc
tttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaaga
aactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttat
cgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctacta
gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctcacacaggacatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcga
agatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggc
tggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgag
ttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaag
cggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggcctt
cgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatc
tgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatt
tgggtcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaataaatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcga
agatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggc
tggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgag
ttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaag
cggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggcctt
cgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatc
tgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatt
tgggtcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaataaatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcga
agatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggc
tggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgag
ttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaag
cggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggcctt
cgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatc
tgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatt
tgggtcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaataa
aaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagt
tcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccct
cgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtc
caggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagg
gcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacgg
catcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtg
ctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccg
ccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt
ttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata</sbh:mutableProvenance>
    <sbh:topLevel rdf:resource="https://synbiohub.org/public/igem/BBa_J24009/1"/>
    <sbh:ownedBy rdf:resource="https://synbiohub.org/user/james"/>
    <sbh:ownedBy rdf:resource="https://synbiohub.org/user/myers"/>
    <sbh:mutableDescription>This part uses a pLac promoter to create motB and also uses an inverter (cI and pcI) to produce LacI.  In addition LasI is produced in the motile state, which creates the signalling molecule AI-1.  LasR is a transcription factor created in the immobile state.  AI-1 and LasR form a complex to initiate transcription from pLasR promoter.  We call this the Freeze Machine.</sbh:mutableDescription>
    <igem:discontinued>false</igem:discontinued>
    <igem:dominant>false</igem:dominant>
    <igem:group_u_list>_58_</igem:group_u_list>
    <igem:m_user_id>0</igem:m_user_id>
    <igem:owner_id>1016</igem:owner_id>
    <igem:owning_group_id>58</igem:owning_group_id>
    <igem:sampleStatus>No part sequence</igem:sampleStatus>
    <igem:status rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#status/Planning"/>
    <sbh:bookmark>false</sbh:bookmark>
    <sbh:mutableNotes>Still planning.</sbh:mutableNotes>
    <sbh:star>false</sbh:star>
    <dc:creator>Jamie Lemon</dc:creator>
    <sbol:type rdf:resource="http://www.biopax.org/release/biopax-level3.owl#DnaRegion"/>
    <sbol:role rdf:resource="http://identifiers.org/so/SO:0000804"/>
    <sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#partType/Composite"/>
  </sbol:ComponentDefinition>
  <prov:Activity rdf:about="https://synbiohub.org/public/igem/igem2sbol/1">
    <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/igem2sbol"/>
    <sbol:displayId>igem2sbol</sbol:displayId>
    <sbol:version>1</sbol:version>
    <dcterms:title>iGEM to SBOL conversion</dcterms:title>
    <dcterms:description>Conversion of the iGEM parts registry to SBOL2.1</dcterms:description>
    <sbh:topLevel rdf:resource="https://synbiohub.org/public/igem/igem2sbol/1"/>
    <sbh:ownedBy rdf:resource="https://synbiohub.org/user/james"/>
    <sbh:ownedBy rdf:resource="https://synbiohub.org/user/myers"/>
    <dc:creator>Chris J. Myers</dc:creator>
    <dc:creator>James Alastair McLaughlin</dc:creator>
    <prov:endedAtTime>2017-03-06T15:00:00.000Z</prov:endedAtTime>
  </prov:Activity>
</rdf:RDF>