LOCUS BBa_J24016 4526 bp DNA linear 12-May-2024 DEFINITION Toggle Switch to Immobilize E. Coli ACCESSION BBa_J24016 VERSION BBa_J24016.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..219 /label=RBS-4\Weaker RBS 215..218 /label=RBS misc_feature 209..219 /label=BBa_B0033 RBS 209..219 /label=BBa_B0033 CDS 226..828 /label=LasI misc_feature 829..861 /label=LVA misc_feature 868..892 /label=Help:Barcodes CDS 226..892 /label=BBa_C0078 CDS 901..1837 /label=BBa_S03271 misc_feature 1852..1855 /label=conserved RBS 1846..1859 /label=BBa_B0031 CDS 1869..2576 /label=cI lambda misc_feature 2577..2609 /label=LVA misc_feature 2616..2640 /label=Help:Barcodes CDS 1866..2615 /label=BBa_C0051 stem_loop 2660..2703 /label=stem_loop misc_feature 2649..2728 /label=BBa_B0010 terminator 2649..2728 /label=BBa_B0010 stem_loop 2744..2763 /label=T7 TE misc_feature 2770..2770 /label=stop polyA_site 2764..2777 /label=polya misc_feature 2737..2777 /label=BBa_B0012 terminator 2737..2777 /label=BBa_B0012 promoter 2823..2828 /label=-10 promoter 2800..2805 /label=-35 misc_feature 2810..2826 /label=OR1 misc_feature 2786..2802 /label=OR2 misc_feature 2786..2834 /label=BBa_R0051 promoter 2786..2834 /label=BBa_R0051 misc_feature 1846..2834 /label=BBa_Q01510 RBS 2843..2853 /label=RBS-4\Weaker RBS 2849..2852 /label=RBS misc_feature 2843..2853 /label=BBa_B0033 RBS 2843..2853 /label=BBa_B0033 CDS 2860..3576 /label=lasR misc_feature 3577..3609 /label=LVA misc_feature 3339..3339 /label=G misc_feature 3616..3640 /label=Help:Barcodes CDS 2860..3640 /label=BBa_C0079 misc_feature 3653..3656 /label=conserved RBS 3649..3660 /label=BBa_B0034 CDS 3667..4389 /label=BBa_E0020 stem_loop 4409..4452 /label=stem_loop misc_feature 4398..4477 /label=BBa_B0010 terminator 4398..4477 /label=BBa_B0010 stem_loop 4493..4512 /label=T7 TE misc_feature 4519..4519 /label=stop polyA_site 4513..4526 /label=polya misc_feature 4486..4526 /label=BBa_B0012 terminator 4486..4526 /label=BBa_B0012 misc_feature 3649..4526 /label=BBa_E0420 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagtc acacaggact actagatgat cgttcagatc 241 ggtcgtcgtg aagagttcga caaaaaactg ctgggtgaaa tgcacaaact gcgtgctcag 301 gttttcaaag aacgtaaagg ttgggacgtt tccgttatcg acgaaatgga aatcgacggt 361 tacgacgctc tgtccccgta ctacatgctg atccaggaag acaccccgga agctcaggtt 421 ttcggttgct ggcgtatctt cgacaccacc ggtccgtaca tgctgaaaaa caccttcccg 481 gaactgctgc acggtaaaga agctccgtgc tccccgcaca tctgggaact gtcccgtttc 541 gctatcaact ccggtcagaa aggttccctg ggtttctccg actgcaccct ggaagctatg 601 cgtgctctgg ctcgttactc cttgcagaac gacatccaga ccctggttac cgttaccacc 661 gttggtgttg aaaaaatgat gatccgtgct ggtctggacg tttcccgttt cggtccgcac 721 ctgaaaatcg gtatcgaacg tgctgttgct ctgcgtatcg aactgaacgc taaaacccag 781 atcgctctgt acggtggtgt tctggttgaa cagcgtctgg ctgtttccgc tgctaacgac 841 gaaaactacg ctctggttgc ttaataactc tgatagtgct agtgtagatc tctactagag 901 ccgaggaagc atgaagaatc aagcgcatcc gattattgtc gtcaaacgac gcaaagccaa 961 aagccacggg gcagcacatg gatcgtggaa gattgcttat gccgacttta tgactgcgat 1021 gatggccttt tttctggtga tgtggctgat ctccatctcc agcccaaaag agctgattca 1081 gattgcggag tacttccgga ctccactggc gactgcggtt acgggcggcg atcgcatttc 1141 taatagtgaa agcccaattc ccggcggtgg tgatgattac acccaaagcc agggggaagt 1201 gaataagcag ccgaacatcg aagagctgaa aaaacgcatg gagcaaagtc gattgcggaa 1261 attgcggggt gatctcgacc agttgataga gtccgatccg aaactgcggg cgttacgtcc 1321 ccatctcaaa atcgatctgg tccaggaagg tctacgtatt cagatcatcg atagccagaa 1381 tcgcccgatg tttagaaccg gcagtgccga tgtcgaaccc tatatgcgcg acattctgcg 1441 cgccattgcg cctgtactga acggtattcc caaccgtatt agcctttcag gtcataccga 1501 tgatttcccc tacgccagcg gtgagaaagg atatagcaac tgggagcttt ctgccgatcg 1561 ggccaatgca tcccgccgcg aactgatggt cggagggttg gatagcggca aagtgttacg 1621 tgtcgtcggc atggcggcaa cgatgcgctt aagcgatcgc ggacctgatg atgccgtcaa 1681 ccgtcgcatc agcctgctgg tactgaacaa acaagccgaa caggccattt tgcatgaaaa 1741 cgccgaaagc cagaatgagc cagtaagcgc cctggaaaaa cctgaggttg caccacaggt 1801 cagtgttccc acaatgccat cagccgaacc gaggtgatac tagagtcaca caggaaacct 1861 actagatgag cacaaaaaag aaaccattaa cacaagagca gcttgaggac gcacgtcgcc 1921 ttaaagcaat ttatgaaaaa aagaaaaatg aacttggctt atcccaggaa tctgtcgcag 1981 acaagatggg gatggggcag tcaggcgttg gtgctttatt taatggcatc aatgcattaa 2041 atgcttataa cgccgcattg cttgcaaaaa ttctcaaagt tagcgttgaa gaatttagcc 2101 cttcaatcgc cagagaaatc tacgagatgt atgaagcggt tagtatgcag ccgtcactta 2161 gaagtgagta tgagtaccct gttttttctc atgttcaggc agggatgttc tcacctgagc 2221 ttagaacctt taccaaaggt gatgcggaga gatgggtaag cacaaccaaa aaagccagtg 2281 attctgcatt ctggcttgag gttgaaggta attccatgac cgcaccaaca ggctccaagc 2341 caagctttcc tgacggaatg ttaattctcg ttgaccctga gcaggctgtt gagccaggtg 2401 atttctgcat agccagactt gggggtgatg agtttacctt caagaaactg atcagggata 2461 gcggtcaggt gtttttacaa ccactaaacc cacagtaccc aatgatccca tgcaatgaga 2521 gttgttccgt tgtggggaaa gttatcgcta gtcagtggcc tgaagagacg tttggcgctg 2581 caaacgacga aaactacgct ttagtagctt aataacgctg atagtgctag tgtagatcgc 2641 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 2701 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 2761 gcctttctgc gtttatatac tagagtaaca ccgtgcgtgt tgactatttt acctctggcg 2821 gtgataatgg ttgctactag agtcacacag gactactaga tggccttggt tgacggtttt 2881 cttgagctgg aacgctcaag tggaaaattg gagtggagcg ccatcctcca gaagatggcg 2941 agcgaccttg gattctcgaa gatcctgttc ggcctgttgc ctaaggacag ccaggactac 3001 gagaacgcct tcatcgtcgg caactacccg gccgcctggc gcgagcatta cgaccgggct 3061 ggctacgcgc gggtcgaccc gacggtcagt cactgtaccc agagcgtact gccgattttc 3121 tgggaaccgt ccatctacca gacgcgaaag cagcacgagt tcttcgagga agcctcggcc 3181 gccggcctgg tgtatgggct gaccatgccg ctgcatggtg ctcgcggcga actcggcgcg 3241 ctgagcctca gcgtggaagc ggaaaaccgg gccgaggcca accgtttcat agagtcggtc 3301 ctgccgaccc tgtggatgct caaggactac gcactgcaaa gcggtgccgg actggccttc 3361 gaacatccgg tcagcaaacc ggtggttctg accagccggg agaaggaagt gttgcagtgg 3421 tgcgccatcg gcaagaccag ttgggagata tcggttatct gcaactgctc ggaagccaat 3481 gtgaacttcc atatgggaaa tattcggcgg aagttcggtg tgacctcccg ccgcgtagcg 3541 gccattatgg ccgttaattt gggtcttatt actctcgctg caaacgacga aaactacgct 3601 ttagtagctt aataacactg atagtgctag tgtagatcac tactagagaa agaggagaaa 3661 tactagatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag 3721 ctggacggcg acgtgaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc 3781 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg 3841 cccaccctcg tgaccaccct gacctggggc gtgcagtgct tcagccgcta ccccgaccac 3901 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc 3961 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac 4021 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg 4081 gggcacaagc tggagtacaa ctacatcagc cacaacgtct atatcaccgc cgacaagcag 4141 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag 4201 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac 4261 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac 4321 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac 4381 aagtaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 4441 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 4501 ttcgggtggg cctttctgcg tttata //