LOCUS BBa_J24666 6546 bp DNA linear 12-May-2024 DEFINITION Toggle Switch to Immobilize E. Coli ACCESSION BBa_J24666 VERSION BBa_J24666.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..219 /label=RBS-4\Weaker RBS 215..218 /label=RBS misc_feature 209..219 /label=BBa_B0033 RBS 209..219 /label=BBa_B0033 CDS 226..828 /label=LasI misc_feature 829..861 /label=LVA misc_feature 868..892 /label=Help:Barcodes CDS 226..892 /label=BBa_C0078 CDS 901..1837 /label=BBa_S03271 misc_feature 1852..1855 /label=conserved RBS 1846..1859 /label=BBa_B0031 CDS 1869..2576 /label=cI lambda misc_feature 2577..2609 /label=LVA misc_feature 2616..2640 /label=Help:Barcodes CDS 1866..2615 /label=BBa_C0051 stem_loop 2660..2703 /label=stem_loop misc_feature 2649..2728 /label=BBa_B0010 terminator 2649..2728 /label=BBa_B0010 stem_loop 2744..2763 /label=T7 TE misc_feature 2770..2770 /label=stop polyA_site 2764..2777 /label=polya misc_feature 2737..2777 /label=BBa_B0012 terminator 2737..2777 /label=BBa_B0012 promoter 2823..2828 /label=-10 promoter 2800..2805 /label=-35 misc_feature 2810..2826 /label=OR1 misc_feature 2786..2802 /label=OR2 misc_feature 2786..2834 /label=BBa_R0051 promoter 2786..2834 /label=BBa_R0051 misc_feature 1846..2834 /label=BBa_Q01510 RBS 2843..2853 /label=RBS-4\Weaker RBS 2849..2852 /label=RBS misc_feature 2843..2853 /label=BBa_B0033 RBS 2843..2853 /label=BBa_B0033 misc_feature 3949..3987 /label=LVA CDS 2860..3987 /label=lacI-LVA misc_feature 2860..3987 /label=BBa_C0012 misc_feature 3988..4012 /label=Help:Barcodes CDS 2860..3987 /label=BBa_C0012 RBS 4021..4033 /label=RBS-3\Weak RBS 4027..4030 /label=RBS misc_feature 4021..4033 /label=BBa_B0032 RBS 4021..4033 /label=BBa_B0032 CDS 4040..4756 /label=lasR misc_feature 4757..4789 /label=LVA misc_feature 4519..4519 /label=G misc_feature 4796..4820 /label=Help:Barcodes CDS 4040..4820 /label=BBa_C0079 misc_feature 4833..4836 /label=conserved RBS 4829..4840 /label=BBa_B0034 misc_feature 4847..4849 /label=start misc_feature 5630..5635 /label=2 misc_feature 5597..5629 /label=LVA CDS 4847..5596 /label=aiiA misc_feature 4847..5635 /label=BBa_C0060 misc_feature 5636..5660 /label=Help:Barcodes CDS 4847..5635 /label=BBa_C0060 misc_feature 4829..5660 /label=BBa_C0260 misc_feature 5673..5676 /label=conserved RBS 5669..5680 /label=BBa_B0034 CDS 5687..6409 /label=BBa_E0020 stem_loop 6429..6472 /label=stem_loop misc_feature 6418..6497 /label=BBa_B0010 terminator 6418..6497 /label=BBa_B0010 stem_loop 6513..6532 /label=T7 TE misc_feature 6539..6539 /label=stop polyA_site 6533..6546 /label=polya misc_feature 6506..6546 /label=BBa_B0012 terminator 6506..6546 /label=BBa_B0012 misc_feature 5669..6546 /label=BBa_E0420 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagtc acacaggact actagatgat cgttcagatc 241 ggtcgtcgtg aagagttcga caaaaaactg ctgggtgaaa tgcacaaact gcgtgctcag 301 gttttcaaag aacgtaaagg ttgggacgtt tccgttatcg acgaaatgga aatcgacggt 361 tacgacgctc tgtccccgta ctacatgctg atccaggaag acaccccgga agctcaggtt 421 ttcggttgct ggcgtatctt cgacaccacc ggtccgtaca tgctgaaaaa caccttcccg 481 gaactgctgc acggtaaaga agctccgtgc tccccgcaca tctgggaact gtcccgtttc 541 gctatcaact ccggtcagaa aggttccctg ggtttctccg actgcaccct ggaagctatg 601 cgtgctctgg ctcgttactc cttgcagaac gacatccaga ccctggttac cgttaccacc 661 gttggtgttg aaaaaatgat gatccgtgct ggtctggacg tttcccgttt cggtccgcac 721 ctgaaaatcg gtatcgaacg tgctgttgct ctgcgtatcg aactgaacgc taaaacccag 781 atcgctctgt acggtggtgt tctggttgaa cagcgtctgg ctgtttccgc tgctaacgac 841 gaaaactacg ctctggttgc ttaataactc tgatagtgct agtgtagatc tctactagag 901 ccgaggaagc atgaagaatc aagcgcatcc gattattgtc gtcaaacgac gcaaagccaa 961 aagccacggg gcagcacatg gatcgtggaa gattgcttat gccgacttta tgactgcgat 1021 gatggccttt tttctggtga tgtggctgat ctccatctcc agcccaaaag agctgattca 1081 gattgcggag tacttccgga ctccactggc gactgcggtt acgggcggcg atcgcatttc 1141 taatagtgaa agcccaattc ccggcggtgg tgatgattac acccaaagcc agggggaagt 1201 gaataagcag ccgaacatcg aagagctgaa aaaacgcatg gagcaaagtc gattgcggaa 1261 attgcggggt gatctcgacc agttgataga gtccgatccg aaactgcggg cgttacgtcc 1321 ccatctcaaa atcgatctgg tccaggaagg tctacgtatt cagatcatcg atagccagaa 1381 tcgcccgatg tttagaaccg gcagtgccga tgtcgaaccc tatatgcgcg acattctgcg 1441 cgccattgcg cctgtactga acggtattcc caaccgtatt agcctttcag gtcataccga 1501 tgatttcccc tacgccagcg gtgagaaagg atatagcaac tgggagcttt ctgccgatcg 1561 ggccaatgca tcccgccgcg aactgatggt cggagggttg gatagcggca aagtgttacg 1621 tgtcgtcggc atggcggcaa cgatgcgctt aagcgatcgc ggacctgatg atgccgtcaa 1681 ccgtcgcatc agcctgctgg tactgaacaa acaagccgaa caggccattt tgcatgaaaa 1741 cgccgaaagc cagaatgagc cagtaagcgc cctggaaaaa cctgaggttg caccacaggt 1801 cagtgttccc acaatgccat cagccgaacc gaggtgatac tagagtcaca caggaaacct 1861 actagatgag cacaaaaaag aaaccattaa cacaagagca gcttgaggac gcacgtcgcc 1921 ttaaagcaat ttatgaaaaa aagaaaaatg aacttggctt atcccaggaa tctgtcgcag 1981 acaagatggg gatggggcag tcaggcgttg gtgctttatt taatggcatc aatgcattaa 2041 atgcttataa cgccgcattg cttgcaaaaa ttctcaaagt tagcgttgaa gaatttagcc 2101 cttcaatcgc cagagaaatc tacgagatgt atgaagcggt tagtatgcag ccgtcactta 2161 gaagtgagta tgagtaccct gttttttctc atgttcaggc agggatgttc tcacctgagc 2221 ttagaacctt taccaaaggt gatgcggaga gatgggtaag cacaaccaaa aaagccagtg 2281 attctgcatt ctggcttgag gttgaaggta attccatgac cgcaccaaca ggctccaagc 2341 caagctttcc tgacggaatg ttaattctcg ttgaccctga gcaggctgtt gagccaggtg 2401 atttctgcat agccagactt gggggtgatg agtttacctt caagaaactg atcagggata 2461 gcggtcaggt gtttttacaa ccactaaacc cacagtaccc aatgatccca tgcaatgaga 2521 gttgttccgt tgtggggaaa gttatcgcta gtcagtggcc tgaagagacg tttggcgctg 2581 caaacgacga aaactacgct ttagtagctt aataacgctg atagtgctag tgtagatcgc 2641 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 2701 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 2761 gcctttctgc gtttatatac tagagtaaca ccgtgcgtgt tgactatttt acctctggcg 2821 gtgataatgg ttgctactag agtcacacag gactactaga tggtgaatgt gaaaccagta 2881 acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg 2941 aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag 3001 ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt 3061 ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa 3121 tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc 3181 gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt 3241 aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg 3301 gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa 3361 gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg 3421 ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat 3481 ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc 3541 ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt 3601 gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt 3661 ggtgcggata tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg 3721 ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg 3781 ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg 3841 aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat 3901 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcaggc tgcaaacgac 3961 gaaaactacg ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag 4021 tcacacagga aagtactaga tggccttggt tgacggtttt cttgagctgg aacgctcaag 4081 tggaaaattg gagtggagcg ccatcctcca gaagatggcg agcgaccttg gattctcgaa 4141 gatcctgttc ggcctgttgc ctaaggacag ccaggactac gagaacgcct tcatcgtcgg 4201 caactacccg gccgcctggc gcgagcatta cgaccgggct ggctacgcgc gggtcgaccc 4261 gacggtcagt cactgtaccc agagcgtact gccgattttc tgggaaccgt ccatctacca 4321 gacgcgaaag cagcacgagt tcttcgagga agcctcggcc gccggcctgg tgtatgggct 4381 gaccatgccg ctgcatggtg ctcgcggcga actcggcgcg ctgagcctca gcgtggaagc 4441 ggaaaaccgg gccgaggcca accgtttcat agagtcggtc ctgccgaccc tgtggatgct 4501 caaggactac gcactgcaaa gcggtgccgg actggccttc gaacatccgg tcagcaaacc 4561 ggtggttctg accagccggg agaaggaagt gttgcagtgg tgcgccatcg gcaagaccag 4621 ttgggagata tcggttatct gcaactgctc ggaagccaat gtgaacttcc atatgggaaa 4681 tattcggcgg aagttcggtg tgacctcccg ccgcgtagcg gccattatgg ccgttaattt 4741 gggtcttatt actctcgctg caaacgacga aaactacgct ttagtagctt aataacactg 4801 atagtgctag tgtagatcac tactagagaa agaggagaaa tactagatga cagtaaagaa 4861 gctttatttc gtcccagcag gtcgttgtat gttggatcat tcgtctgtta atagtacatt 4921 aacaccagga gaattattag acttaccggt ttggtgttat cttttggaga ctgaagaagg 4981 acctatttta gtagatacag gtatgccaga aagtgcagtt aataatgaag gtctttttaa 5041 cggtacattt gtcgaagggc aggttttacc gaaaatgact gaagaagata gaatcgtgaa 5101 tattttaaaa cgggttggtt atgagccgga agaccttctt tatattatta gttctcactt 5161 gcattttgat catgcaggag gaaatggcgc ttttataaat acaccaatca ttgtacagcg 5221 tgctgaatat gaggcggcgc agcatagcga agaatatttg aaagaatgta tattgccgaa 5281 tttaaactac aaaatcattg aaggtgatta tgaagtcgta ccaggagttc aattattgca 5341 tacaccaggc catactccag ggcatcaatc gctattaatt gagacagaaa aatccggtcc 5401 tgtattatta acgattgatg catcgtatac gaaagagaat tttgaaaatg aagtgccatt 5461 tgcgggattt gattcagaat tagctttatc ttcaattaaa cgtttaaaag aagtggtgat 5521 gaaagagaag ccgattgttt tctttggaca tgatatagag caggaaaggg gatgtaaagt 5581 gttccctgaa tatatagctg caaacgacga aaactacgct ttagtagctt aataacgctg 5641 atagtgctag tgtagatcgc tactagagaa agaggagaaa tactagatgg tgagcaaggg 5701 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtgaacgg 5761 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct 5821 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct 5881 gacctggggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt 5941 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg 6001 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga 6061 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa 6121 ctacatcagc cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa 6181 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca 6241 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca 6301 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt 6361 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaataat actagagcca 6421 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 6481 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 6541 tttata //