##gff-version 3
##sequence-region BBa_J24666 1 6546
BBa_J24666	.	CDS	4040	4820	.	+	0	ID=BBa_C0079;Name=BBa_C0079
BBa_J24666	.	engineered_tag	4796	4820	.	+	0	ID=annotation2213999;Name=Help:Barcodes;Parent=BBa_C0079
BBa_J24666	.	sequence_alteration	4519	4519	.	+	0	ID=annotation308006;Name=G;Parent=BBa_C0079
BBa_J24666	.	tag	4757	4789	.	+	0	ID=annotation307948;Name=LVA;Parent=BBa_C0079
BBa_J24666	.	CDS	4040	4756	.	+	0	ID=annotation307935;Name=lasR;Parent=BBa_C0079
BBa_J24666	.	CDS	901	1837	.	+	0	ID=BBa_S03271;Name=BBa_S03271
BBa_J24666	.	engineered_region	1846	2834	.	+	0	ID=BBa_Q01510;Name=BBa_Q01510
BBa_J24666	.	terminator	2737	2777	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q01510
BBa_J24666	.	polyA_site	2764	2777	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24666	.	engineered_region	2737	2777	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24666	.	stem_loop	2744	2763	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J24666	.	stop_codon	2770	2770	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J24666	.	ribosome_entry_site	1846	1859	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_Q01510
BBa_J24666	.	conserved_region	1852	1855	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_J24666	.	promoter	2786	2834	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_Q01510
BBa_J24666	.	non_covalent_binding_site	2810	2826	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_J24666	.	promoter	2823	2828	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_J24666	.	non_covalent_binding_site	2786	2802	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_J24666	.	engineered_region	2786	2834	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_J24666	.	promoter	2800	2805	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_J24666	.	terminator	2649	2728	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q01510
BBa_J24666	.	engineered_region	2649	2728	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J24666	.	stem_loop	2660	2703	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24666	.	CDS	1866	2615	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_Q01510
BBa_J24666	.	tag	2577	2609	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_J24666	.	CDS	1869	2576	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_J24666	.	engineered_tag	2616	2640	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_J24666	.	ribosome_entry_site	4021	4033	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_J24666	.	ribosome_entry_site	4021	4033	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J24666	.	ribosome_entry_site	4027	4030	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J24666	.	engineered_region	4021	4033	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J24666	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_J24666	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J24666	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J24666	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J24666	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J24666	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J24666	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J24666	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J24666	.	engineered_region	4829	5660	.	+	0	ID=BBa_C0260;Name=BBa_C0260
BBa_J24666	.	ribosome_entry_site	4829	4840	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0260
BBa_J24666	.	conserved_region	4833	4836	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24666	.	CDS	4847	5635	.	+	0	ID=BBa_C0060;Name=BBa_C0060;Parent=BBa_C0260
BBa_J24666	.	engineered_region	4847	5635	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_J24666	.	tag	5597	5629	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_J24666	.	start_codon	4847	4849	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_J24666	.	stop_codon	5630	5635	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_J24666	.	engineered_tag	5636	5660	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_J24666	.	CDS	4847	5596	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_J24666	.	engineered_region	5669	6546	.	+	0	ID=BBa_E0420;Name=BBa_E0420
BBa_J24666	.	terminator	6506	6546	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0420
BBa_J24666	.	polyA_site	6533	6546	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24666	.	engineered_region	6506	6546	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24666	.	stem_loop	6513	6532	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J24666	.	stop_codon	6539	6539	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J24666	.	terminator	6418	6497	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0420
BBa_J24666	.	engineered_region	6418	6497	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J24666	.	stem_loop	6429	6472	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24666	.	CDS	5687	6409	.	+	0	ID=BBa_E0020;Name=BBa_E0020;Parent=BBa_E0420
BBa_J24666	.	ribosome_entry_site	5669	5680	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0420
BBa_J24666	.	conserved_region	5673	5676	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24666	.	CDS	2860	3987	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_J24666	.	CDS	2860	3987	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J24666	.	engineered_tag	3988	4012	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J24666	.	tag	3949	3987	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J24666	.	engineered_region	2860	3987	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J24666	.	ribosome_entry_site	209	219	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_J24666	.	engineered_region	209	219	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_J24666	.	ribosome_entry_site	209	219	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_J24666	.	ribosome_entry_site	215	218	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_J24666	.	ribosome_entry_site	2843	2853	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_J24666	.	engineered_region	2843	2853	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_J24666	.	ribosome_entry_site	2843	2853	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_J24666	.	ribosome_entry_site	2849	2852	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_J24666	.	CDS	226	892	.	+	0	ID=BBa_C0078;Name=BBa_C0078
BBa_J24666	.	tag	829	861	.	+	0	ID=annotation306608;Name=LVA;Parent=BBa_C0078
BBa_J24666	.	CDS	226	828	.	+	0	ID=annotation305970;Name=LasI;Parent=BBa_C0078
BBa_J24666	.	engineered_tag	868	892	.	+	0	ID=annotation2214003;Name=Help:Barcodes;Parent=BBa_C0078
>BBa_J24666
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggactactagatgatcgttcag
atcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgta
aaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatcca
ggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacacc
ttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccg
gtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacga
catccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttc
ggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgt
acggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctggttgcttaataact
ctgatagtgctagtgtagatctctactagagccgaggaagcatgaagaatcaagcgcatccgattattgtcgtcaaacg
acgcaaagccaaaagccacggggcagcacatggatcgtggaagattgcttatgccgactttatgactgcgatgatggcc
ttttttctggtgatgtggctgatctccatctccagcccaaaagagctgattcagattgcggagtacttccggactccac
tggcgactgcggttacgggcggcgatcgcatttctaatagtgaaagcccaattcccggcggtggtgatgattacaccca
aagccagggggaagtgaataagcagccgaacatcgaagagctgaaaaaacgcatggagcaaagtcgattgcggaaattg
cggggtgatctcgaccagttgatagagtccgatccgaaactgcgggcgttacgtccccatctcaaaatcgatctggtcc
aggaaggtctacgtattcagatcatcgatagccagaatcgcccgatgtttagaaccggcagtgccgatgtcgaacccta
tatgcgcgacattctgcgcgccattgcgcctgtactgaacggtattcccaaccgtattagcctttcaggtcataccgat
gatttcccctacgccagcggtgagaaaggatatagcaactgggagctttctgccgatcgggccaatgcatcccgccgcg
aactgatggtcggagggttggatagcggcaaagtgttacgtgtcgtcggcatggcggcaacgatgcgcttaagcgatcg
cggacctgatgatgccgtcaaccgtcgcatcagcctgctggtactgaacaaacaagccgaacaggccattttgcatgaa
aacgccgaaagccagaatgagccagtaagcgccctggaaaaacctgaggttgcaccacaggtcagtgttcccacaatgc
catcagccgaaccgaggtgatactagagtcacacaggaaacctactagatgagcacaaaaaagaaaccattaacacaag
agcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgt
cgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgcc
gcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatg
aagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctc
acctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattc
tggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcg
ttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaact
gatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttcc
gttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtag
cttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagac
tgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctt
tctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagtc
acacaggactactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcaga
ccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagct
gaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctg
gccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcga
tggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcat
taactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtc
tctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcat
tgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataa
atatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaacc
atgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcg
ccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgtta
tatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctct
cagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgc
aaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggc
tgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagtcacacagg
aaagtactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctc
cagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacg
ccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggt
cagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttc
gaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctga
gcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaa
ggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggag
aaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatg
tgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaattt
gggtcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatca
ctactagagaaagaggagaaatactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatc
attcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaaga
aggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaa
gggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagacc
ttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgt
acagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaa
atcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgc
tattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatga
agtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaag
ccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacg
aaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagat
ggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaag
ttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagc
tgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaa
gcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaac
tacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaagg
aggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaa
gaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcag
aacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagacc
ccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgta
caagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata