##gff-version 3
##sequence-region BBa_J24667 1 1562
BBa_J24667	.	terminator	1522	1562	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J24667	.	stem_loop	1529	1548	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J24667	.	polyA_site	1549	1562	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24667	.	stop_codon	1555	1555	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J24667	.	engineered_region	1522	1562	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24667	.	ribosome_entry_site	694	705	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J24667	.	conserved_region	698	701	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24667	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J24667	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24667	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J24667	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J24667	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J24667	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J24667	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J24667	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J24667	.	CDS	101	685	.	+	0	ID=BBa_C0161;Name=BBa_C0161
BBa_J24667	.	CDS	101	685	.	+	0	ID=annotation1891615;Name=LuxI;Parent=BBa_C0161
BBa_J24667	.	ribosome_entry_site	83	94	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J24667	.	conserved_region	87	90	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24667	.	engineered_region	712	1425	.	+	0	ID=BBa_J06504;Name=BBa_J06504
BBa_J24667	.	CDS	712	1422	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_J24667	.	sequence_alteration	1063	1063	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_J24667	.	terminator	1434	1513	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J24667	.	stem_loop	1445	1488	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24667	.	engineered_region	1434	1513	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_J24667
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaag
gtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcaga
tgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttatta
cctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaata
tagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaact
atttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcga
tttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttg
tattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaattaataatactagagaaagaggagaaatactag
atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccg
tgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggt
gaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaag
caccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgagg
acggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcac
caacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgag
gacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagacca
cctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacga
ggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaa
taatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcg
gtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata