LOCUS       BBa_J24669              3100 bp    DNA     linear       26-Nov-2024
DEFINITION  Tri-Stable Toggle (Arabinose induced component)
ACCESSION   BBa_J24669
VERSION     BBa_J24669.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     CDS             1240..1856
                     /label=tetR
     misc_feature    1857..1890
                     /label=SsrA
     misc_feature    1897..1921
                     /label=Help:Barcodes
     CDS             1237..1921
                     /label=BBa_C0040
     misc_feature    1219..1921
                     /label=BBa_S03518
     misc_feature    1934..1937
                     /label=conserved
     RBS             1930..1941
                     /label=BBa_B0034
     misc_feature    3037..3075
                     /label=LVA
     CDS             1948..3075
                     /label=lacI-LVA
     misc_feature    1948..3075
                     /label=BBa_C0012
     misc_feature    3076..3100
                     /label=Help:Barcodes
     CDS             1948..3075
                     /label=BBa_C0012
     misc_feature    1930..3100
                     /label=BBa_S03520
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatgt ccagattaga taaaagtaaa
     1261 gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt
     1321 aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag
     1381 cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc
     1441 cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt
     1501 gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa
     1561 cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag
     1621 aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat
     1681 caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca
     1741 ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc cttcttattc
     1801 ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtccgct
     1861 gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca
     1921 ctactagaga aagaggagaa atactagatg gtgaatgtga aaccagtaac gttatacgat
     1981 gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc
     2041 cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt
     2101 cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc
     2161 tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat
     2221 caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa
     2281 gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg
     2341 gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt
     2401 gatgtctctg accagacacc catcaacagt attattttct cccatgaaga cggtacgcga
     2461 ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca
     2521 ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat
     2581 caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa
     2641 accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag
     2701 atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc
     2761 tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc
     2821 atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct
     2881 cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc
     2941 accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     3001 ctggcacgac aggtttcccg actggaaagc gggcaggctg caaacgacga aaactacgct
     3061 ttagtagctt aataactctg atagtgctag tgtagatctc
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