LOCUS       BBa_J24670              1877 bp    DNA     linear       03-May-2024
DEFINITION  Tri-Stable Toggle (Lactose induced component)
ACCESSION   BBa_J24670
VERSION     BBa_J24670.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             230..846
                     /label=tetR
     misc_feature    847..880
                     /label=SsrA
     misc_feature    887..911
                     /label=Help:Barcodes
     CDS             227..911
                     /label=BBa_C0040
     misc_feature    209..911
                     /label=BBa_S03518
     misc_feature    924..927
                     /label=conserved
     RBS             920..931
                     /label=BBa_B0034
     CDS             938..1813
                     /label=araC
     misc_feature    1814..1846
                     /label=LVA
     misc_feature    1853..1877
                     /label=Help:Barcodes
     CDS             938..1877
                     /label=BBa_C0080
     misc_feature    920..1877
                     /label=BBa_S03519
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt ccagattaga
      241 taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt
      301 aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt
      361 aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac
      421 tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag
      481 ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc
      541 tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt
      601 ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt
      661 attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag
      721 tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc
      781 cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag
      841 tgggtccgct gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta
      901 gtgtagatca ctactagaga aagaggagaa atactagatg gctgaagcgc aaaatgatcc
      961 cctgctgccg ggatactcgt ttaacgccca tctggtggcg ggtttaacgc cgattgaggc
     1021 caatggttat ctcgattttt ttatcgaccg accgctggga atgaaaggtt atattctcaa
     1081 tctcaccatt cgcggtcagg gggtggtgaa aaatcaggga cgagaatttg tctgccgacc
     1141 gggtgatatt ttgctgttcc cgccaggaga gattcatcac tacggtcgtc atccggaggc
     1201 tcgcgaatgg tatcaccagt gggtttactt tcgtccgcgc gcctactggc atgaatggct
     1261 taactggccg tcaatatttg ccaatacggg tttctttcgc ccggatgaag cgcaccagcc
     1321 gcatttcagc gacctgtttg ggcaaatcat taacgccggg caaggggaag ggcgctattc
     1381 ggagctgctg gcgataaatc tgcttgagca attgttactg cggcgcatgg aagcgattaa
     1441 cgagtcgctc catccaccga tggataatcg ggtacgcgag gcttgtcagt acatcagcga
     1501 tcacctggca gacagcaatt ttgatatcgc cagcgtcgca cagcatgttt gcctgtcgcc
     1561 gtcgcgtctg tcacatcttt tccgccagca gttagggatt agcgtcttaa gctggcgcga
     1621 ggaccaacgc atcagccagg cgaagctgct tttgagcact acccggatgc ctatcgccac
     1681 cgtcggtcgc aatgttggtt ttgacgatca actctatttc tcgcgagtat ttaaaaaatg
     1741 caccggggcc agcccgagcg agttccgtgc cggttgtgaa gaaaaagtga atgatgtagc
     1801 cgtcaagttg tcagctgcaa acgacgaaaa ctacgcttta gtagcttaat aacactgata
     1861 gtgctagtgt agatcac
//