LOCUS       BBa_J24671              2199 bp    DNA     linear       14-May-2024
DEFINITION  Tri-Stable Toggle (Tetracycline induced component)
ACCESSION   BBa_J24671
VERSION     BBa_J24671.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    1170..1208
                     /label=LVA
     CDS             81..1208
                     /label=lacI-LVA
     misc_feature    81..1208
                     /label=BBa_C0012
     misc_feature    1209..1233
                     /label=Help:Barcodes
     CDS             81..1208
                     /label=BBa_C0012
     misc_feature    63..1233
                     /label=BBa_S03520
     misc_feature    1246..1249
                     /label=conserved
     RBS             1242..1253
                     /label=BBa_B0034
     CDS             1260..2135
                     /label=araC
     misc_feature    2136..2168
                     /label=LVA
     misc_feature    2175..2199
                     /label=Help:Barcodes
     CDS             1260..2199
                     /label=BBa_C0080
     misc_feature    1242..2199
                     /label=BBa_S03519
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag
      121 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt
      181 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc
      241 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc
      301 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg
      361 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg
      421 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc
      481 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct
      541 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg
      601 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt
      661 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc
      721 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc
      781 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc
      841 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag
      901 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac
      961 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc
     1021 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg
     1081 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     1141 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag
     1201 cttaataact ctgatagtgc tagtgtagat ctctactaga gaaagaggag aaatactaga
     1261 tggctgaagc gcaaaatgat cccctgctgc cgggatactc gtttaacgcc catctggtgg
     1321 cgggtttaac gccgattgag gccaatggtt atctcgattt ttttatcgac cgaccgctgg
     1381 gaatgaaagg ttatattctc aatctcacca ttcgcggtca gggggtggtg aaaaatcagg
     1441 gacgagaatt tgtctgccga ccgggtgata ttttgctgtt cccgccagga gagattcatc
     1501 actacggtcg tcatccggag gctcgcgaat ggtatcacca gtgggtttac tttcgtccgc
     1561 gcgcctactg gcatgaatgg cttaactggc cgtcaatatt tgccaatacg ggtttctttc
     1621 gcccggatga agcgcaccag ccgcatttca gcgacctgtt tgggcaaatc attaacgccg
     1681 ggcaagggga agggcgctat tcggagctgc tggcgataaa tctgcttgag caattgttac
     1741 tgcggcgcat ggaagcgatt aacgagtcgc tccatccacc gatggataat cgggtacgcg
     1801 aggcttgtca gtacatcagc gatcacctgg cagacagcaa ttttgatatc gccagcgtcg
     1861 cacagcatgt ttgcctgtcg ccgtcgcgtc tgtcacatct tttccgccag cagttaggga
     1921 ttagcgtctt aagctggcgc gaggaccaac gcatcagcca ggcgaagctg cttttgagca
     1981 ctacccggat gcctatcgcc accgtcggtc gcaatgttgg ttttgacgat caactctatt
     2041 tctcgcgagt atttaaaaaa tgcaccgggg ccagcccgag cgagttccgt gccggttgtg
     2101 aagaaaaagt gaatgatgta gccgtcaagt tgtcagctgc aaacgacgaa aactacgctt
     2161 tagtagctta ataacactga tagtgctagt gtagatcac
//