LOCUS BBa_J24672 9894 bp DNA linear 12-May-2024 DEFINITION Tri-Stable Toggle with Flourescent Reporters ACCESSION BBa_J24672 VERSION BBa_J24672.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1240..1856 /label=tetR misc_feature 1857..1890 /label=SsrA misc_feature 1897..1921 /label=Help:Barcodes CDS 1237..1921 /label=BBa_C0040 misc_feature 1219..1921 /label=BBa_S03518 misc_feature 1934..1937 /label=conserved RBS 1930..1941 /label=BBa_B0034 misc_feature 3037..3075 /label=LVA CDS 1948..3075 /label=lacI-LVA misc_feature 1948..3075 /label=BBa_C0012 misc_feature 3076..3100 /label=Help:Barcodes CDS 1948..3075 /label=BBa_C0012 misc_feature 1930..3100 /label=BBa_S03520 promoter 1..3100 /label=BBa_J24669 misc_feature 3113..3116 /label=conserved RBS 3109..3120 /label=BBa_B0034 CDS 3127..3834 /label=mCherry misc_feature 3478..3478 /label=C->T (removing PstI site) misc_feature 3835..3843 /label=LVA misc_feature 3127..3849 /label=BBa_J06505 stem_loop 3869..3912 /label=stem_loop misc_feature 3858..3937 /label=BBa_B0010 terminator 3858..3937 /label=BBa_B0010 stem_loop 3953..3972 /label=T7 TE misc_feature 3979..3979 /label=stop polyA_site 3973..3986 /label=polya misc_feature 3946..3986 /label=BBa_B0012 terminator 3946..3986 /label=BBa_B0012 misc_feature 3109..3986 /label=BBa_J06703 CDS 3995..4082 /label=end of LacI coding region (inactive) misc_feature 3995..4194 /label=BBa_R0010 misc_feature 4083..4120 /label=CAP binding site promoter 4131..4136 /label=-35 promoter 4155..4160 /label=-10 misc_feature 4160..4194 /label=LacI binding site misc_feature 4167..4167 /label=start promoter 3995..4194 /label=BBa_R0010 misc_feature 4207..4210 /label=conserved RBS 4203..4214 /label=BBa_B0034 CDS 4224..4840 /label=tetR misc_feature 4841..4874 /label=SsrA misc_feature 4881..4905 /label=Help:Barcodes CDS 4221..4905 /label=BBa_C0040 misc_feature 4203..4905 /label=BBa_S03518 misc_feature 4918..4921 /label=conserved RBS 4914..4925 /label=BBa_B0034 CDS 4932..5807 /label=araC misc_feature 5808..5840 /label=LVA misc_feature 5847..5871 /label=Help:Barcodes CDS 4932..5871 /label=BBa_C0080 misc_feature 4914..5871 /label=BBa_S03519 promoter 3995..5871 /label=BBa_J24670 misc_feature 5884..5887 /label=conserved RBS 5880..5891 /label=BBa_B0034 CDS 5898..6659 /label=CFP (LVA) misc_feature 6616..6653 /label=SsrA misc_feature 6654..6659 /label=2 misc_feature 5966..5966 /label=A misc_feature 5898..6659 /label=BBa_E0022 misc_feature 5898..6659 /label=BBa_E0022 stem_loop 6679..6722 /label=stem_loop misc_feature 6668..6747 /label=BBa_B0010 terminator 6668..6747 /label=BBa_B0010 stem_loop 6763..6782 /label=T7 TE misc_feature 6789..6789 /label=stop polyA_site 6783..6796 /label=polya misc_feature 6756..6796 /label=BBa_B0012 terminator 6756..6796 /label=BBa_B0012 misc_feature 5880..6796 /label=BBa_E0422 misc_feature 6805..6823 /label=TetR 1 misc_feature 6805..6858 /label=BBa_R0040 promoter 6824..6829 /label=-35 misc_feature 6830..6848 /label=TetR 2 promoter 6847..6852 /label=-10 promoter 6805..6858 /label=BBa_R0040 misc_feature 6871..6874 /label=conserved RBS 6867..6878 /label=BBa_B0034 misc_feature 7974..8012 /label=LVA CDS 6885..8012 /label=lacI-LVA misc_feature 6885..8012 /label=BBa_C0012 misc_feature 8013..8037 /label=Help:Barcodes CDS 6885..8012 /label=BBa_C0012 misc_feature 6867..8037 /label=BBa_S03520 misc_feature 8050..8053 /label=conserved RBS 8046..8057 /label=BBa_B0034 CDS 8064..8939 /label=araC misc_feature 8940..8972 /label=LVA misc_feature 8979..9003 /label=Help:Barcodes CDS 8064..9003 /label=BBa_C0080 misc_feature 8046..9003 /label=BBa_S03519 promoter 6805..9003 /label=BBa_J24671 misc_feature 9016..9019 /label=conserved RBS 9012..9023 /label=BBa_B0034 CDS 9030..9791 /label=YFP (LVA) misc_feature 9786..9791 /label=2 misc_feature 9747..9785 /label=SsrA misc_feature 9271..9271 /label=A (Q->L) misc_feature 9030..9791 /label=BBa_E0032 misc_feature 9030..9791 /label=BBa_E0032 stem_loop 9807..9826 /label=T7 TE misc_feature 9833..9833 /label=stop polyA_site 9827..9840 /label=polya misc_feature 9800..9840 /label=BBa_B0012 terminator 9800..9840 /label=BBa_B0012 stem_loop 9861..9883 /label=stem_loop misc_feature 9849..9894 /label=BBa_B0011 terminator 9849..9894 /label=BBa_B0011 misc_feature 9012..9894 /label=BBa_E0438 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgt ccagattaga taaaagtaaa 1261 gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt 1321 aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag 1381 cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc 1441 cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt 1501 gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa 1561 cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag 1621 aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat 1681 caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca 1741 ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc cttcttattc 1801 ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtccgct 1861 gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca 1921 ctactagaga aagaggagaa atactagatg gtgaatgtga aaccagtaac gttatacgat 1981 gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc 2041 cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt 2101 cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc 2161 tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat 2221 caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa 2281 gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg 2341 gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt 2401 gatgtctctg accagacacc catcaacagt attattttct cccatgaaga cggtacgcga 2461 ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca 2521 ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat 2581 caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa 2641 accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag 2701 atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc 2761 tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc 2821 atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct 2881 cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc 2941 accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 3001 ctggcacgac aggtttcccg actggaaagc gggcaggctg caaacgacga aaactacgct 3061 ttagtagctt aataactctg atagtgctag tgtagatctc tactagagaa agaggagaaa 3121 tactagatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc 3181 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc 3241 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc 3301 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg 3361 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg 3421 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccttg 3481 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc 3541 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag 3601 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac 3661 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac 3721 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag 3781 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagttagta 3841 gcttaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 3901 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 3961 ttcgggtggg cctttctgcg tttatatact agagcaatac gcaaaccgcc tctccccgcg 4021 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt 4081 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt 4141 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacatactag 4201 agaaagagga gaaatactag atgtccagat tagataaaag taaagtgatt aacagcgcat 4261 tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc 4321 taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg 4381 ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa 4441 gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc 4501 gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg 4561 aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac 4621 tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg 4681 ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta 4741 tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca 4801 tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact 4861 acgctttagt agcttaataa cactgatagt gctagtgtag atcactacta gagaaagagg 4921 agaaatacta gatggctgaa gcgcaaaatg atcccctgct gccgggatac tcgtttaacg 4981 cccatctggt ggcgggttta acgccgattg aggccaatgg ttatctcgat ttttttatcg 5041 accgaccgct gggaatgaaa ggttatattc tcaatctcac cattcgcggt cagggggtgg 5101 tgaaaaatca gggacgagaa tttgtctgcc gaccgggtga tattttgctg ttcccgccag 5161 gagagattca tcactacggt cgtcatccgg aggctcgcga atggtatcac cagtgggttt 5221 actttcgtcc gcgcgcctac tggcatgaat ggcttaactg gccgtcaata tttgccaata 5281 cgggtttctt tcgcccggat gaagcgcacc agccgcattt cagcgacctg tttgggcaaa 5341 tcattaacgc cgggcaaggg gaagggcgct attcggagct gctggcgata aatctgcttg 5401 agcaattgtt actgcggcgc atggaagcga ttaacgagtc gctccatcca ccgatggata 5461 atcgggtacg cgaggcttgt cagtacatca gcgatcacct ggcagacagc aattttgata 5521 tcgccagcgt cgcacagcat gtttgcctgt cgccgtcgcg tctgtcacat cttttccgcc 5581 agcagttagg gattagcgtc ttaagctggc gcgaggacca acgcatcagc caggcgaagc 5641 tgcttttgag cactacccgg atgcctatcg ccaccgtcgg tcgcaatgtt ggttttgacg 5701 atcaactcta tttctcgcga gtatttaaaa aatgcaccgg ggccagcccg agcgagttcc 5761 gtgccggttg tgaagaaaaa gtgaatgatg tagccgtcaa gttgtcagct gcaaacgacg 5821 aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca ctactagaga 5881 aagaggagaa atactagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca 5941 tcctggtcga gctggacggc gacgtgaacg gccacaagtt cagcgtgtcc ggcgagggcg 6001 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc 6061 ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc ttcagccgct 6121 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc 6181 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt 6241 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg 6301 gcaacatcct ggggcacaag ctggagtaca actacatcag ccacaacgtc tatatcaccg 6361 ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg 6421 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc 6481 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga 6541 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg 6601 acgagctgta caagaggcct gctgcaaacg acgaaaacta cgctttagta gcttaataat 6661 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 6721 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 6781 cctttctgcg tttatatact agagtcccta tcagtgatag agattgacat ccctatcagt 6841 gatagagata ctgagcacta ctagagaaag aggagaaata ctagatggtg aatgtgaaac 6901 cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg 6961 tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg 7021 cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc 7081 tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga 7141 ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg 7201 gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga 7261 tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg 7321 ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt attttctccc 7381 atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg 7441 cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata 7501 aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca 7561 tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc 7621 tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc 7681 gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata 7741 tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc 7801 gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac 7861 tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg 7921 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg caggctgcaa 7981 acgacgaaaa ctacgcttta gtagcttaat aactctgata gtgctagtgt agatctctac 8041 tagagaaaga ggagaaatac tagatggctg aagcgcaaaa tgatcccctg ctgccgggat 8101 actcgtttaa cgcccatctg gtggcgggtt taacgccgat tgaggccaat ggttatctcg 8161 atttttttat cgaccgaccg ctgggaatga aaggttatat tctcaatctc accattcgcg 8221 gtcagggggt ggtgaaaaat cagggacgag aatttgtctg ccgaccgggt gatattttgc 8281 tgttcccgcc aggagagatt catcactacg gtcgtcatcc ggaggctcgc gaatggtatc 8341 accagtgggt ttactttcgt ccgcgcgcct actggcatga atggcttaac tggccgtcaa 8401 tatttgccaa tacgggtttc tttcgcccgg atgaagcgca ccagccgcat ttcagcgacc 8461 tgtttgggca aatcattaac gccgggcaag gggaagggcg ctattcggag ctgctggcga 8521 taaatctgct tgagcaattg ttactgcggc gcatggaagc gattaacgag tcgctccatc 8581 caccgatgga taatcgggta cgcgaggctt gtcagtacat cagcgatcac ctggcagaca 8641 gcaattttga tatcgccagc gtcgcacagc atgtttgcct gtcgccgtcg cgtctgtcac 8701 atcttttccg ccagcagtta gggattagcg tcttaagctg gcgcgaggac caacgcatca 8761 gccaggcgaa gctgcttttg agcactaccc ggatgcctat cgccaccgtc ggtcgcaatg 8821 ttggttttga cgatcaactc tatttctcgc gagtatttaa aaaatgcacc ggggccagcc 8881 cgagcgagtt ccgtgccggt tgtgaagaaa aagtgaatga tgtagccgtc aagttgtcag 8941 ctgcaaacga cgaaaactac gctttagtag cttaataaca ctgatagtgc tagtgtagat 9001 cactactaga gaaagaggag aaatactaga tggtgagcaa gggcgaggag ctgttcaccg 9061 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt 9121 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca 9181 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgcaat 9241 gcttcgcccg ctaccccgac cacatgaagc tgcacgactt cttcaagtcc gccatgcccg 9301 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg 9361 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact 9421 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg 9481 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca 9541 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg 9601 acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag 9661 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca 9721 ctctcggcat ggacgagctg tacaagaggc ctgctgcaaa cgacgaaaac tacgctttag 9781 tagcttaata atactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 9841 tactagagag agaatataaa aagccagatt attaatccgg cttttttatt attt //