BBa_J24672
type: ComponentDefinition
persistent identity: BBa J24672
display id: BBa_J24672
version: 1
was generated by: iGEM to SBOL conversion
description: Tri-Stable Toggle with Flourescent Reporters
created: 2006-07-23T11:00:00Z
modified: 2015-08-31T04:08:41Z
mutable provenance: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaa tagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgc gccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaatt gctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagt aacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggct ggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactg gtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccc tgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcct caatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccatt cagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaa agcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcg tcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgttttt ttgggctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaa tcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgcctt agccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgt gctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgcc aacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaa agaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattc ggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacact gatagtgctagtgtagatcactactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatg…
owned by: james
owned by: myers
mutable description: The tri-stable toggle switch can alternate between three gene products through transient induction with either tetracycline, arabinose, or lactose. Following an induction event the system remains at a stable state until another induction event. This project is a conceptual extension of "Construction of a Genetic Toggle in E. Coli" (Gardner, Collins, Nature vol 403 pg 339 - 342)
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 1061
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The flourescent proteins are all tagged with LVA. This may lead to low level of flourescence.
star: false
creator: Azeem Kaka
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_J06703
component: BBa_J24669
component: BBa_E0438
component: BBa_J24671
component: BBa_E0422
component: BBa_J24670
sequence annotation: BBa_J24669
sequence annotation: BBa_J06703
sequence annotation: BBa_J24670
sequence annotation: BBa_E0422
sequence annotation: BBa_J24671
sequence annotation: BBa_E0438
sequence: https://synbiohub.org/public/igem/BBa_J24672_sequence/1

BBa_J06703
type: Component
persistent identity: component2324159
display id: component2324159
version: 1
top level: BBa_J24672
definition: mCherry
access: public

BBa_J24669
type: Component
persistent identity: component2324146
display id: component2324146
version: 1
top level: BBa_J24672
definition: BBa_J24669
access: public

BBa_E0438
type: Component
persistent identity: component2324229
display id: component2324229
version: 1
top level: BBa_J24672
definition: BBa_E0438
access: public

BBa_J24671
type: Component
persistent identity: component2324215
display id: component2324215
version: 1
top level: BBa_J24672
definition: BBa_J24671
access: public

BBa_E0422
type: Component
persistent identity: component2324195
display id: component2324195
version: 1
top level: BBa_J24672
definition: BBa_E0422
access: public

BBa_J24670
type: Component
persistent identity: component2324181
display id: component2324181
version: 1
top level: BBa_J24672
definition: BBa_J24670
access: public

BBa_J24669
type: SequenceAnnotation
persistent identity: annotation2324146
display id: annotation2324146
version: 1
top level: BBa_J24672
location: https://synbiohub.org/public/igem/BBa_J24672/annotation2324146/range2324146/1
component: BBa_J24669

https://synbiohub.org/public/igem/BBa_J24672/annotation2324146/range2324146/1
type: Range
persistent identity: range2324146
display id: range2324146
version: 1
top level: BBa_J24672
direction: direction/reverse
start: 1
end: 3100
orientation: inline

BBa_J06703
type: SequenceAnnotation
persistent identity: annotation2324159
display id: annotation2324159
version: 1
top level: BBa_J24672
location: https://synbiohub.org/public/igem/BBa_J24672/annotation2324159/range2324159/1
component: BBa_J06703

https://synbiohub.org/public/igem/BBa_J24672/annotation2324159/range2324159/1
type: Range
persistent identity: range2324159
display id: range2324159
version: 1
top level: BBa_J24672
direction: direction/reverse
start: 3109
end: 3986
orientation: inline

BBa_J24670
type: SequenceAnnotation
persistent identity: annotation2324181
display id: annotation2324181
version: 1
top level: BBa_J24672
location: https://synbiohub.org/public/igem/BBa_J24672/annotation2324181/range2324181/1
component: BBa_J24670

https://synbiohub.org/public/igem/BBa_J24672/annotation2324181/range2324181/1
type: Range
persistent identity: range2324181
display id: range2324181
version: 1
top level: BBa_J24672
direction: direction/reverse
start: 3995
end: 5871
orientation: inline

BBa_E0422
type: SequenceAnnotation
persistent identity: annotation2324195
display id: annotation2324195
version: 1
top level: BBa_J24672
location: https://synbiohub.org/public/igem/BBa_J24672/annotation2324195/range2324195/1
component: BBa_E0422

https://synbiohub.org/public/igem/BBa_J24672/annotation2324195/range2324195/1
type: Range
persistent identity: range2324195
display id: range2324195
version: 1
top level: BBa_J24672
direction: direction/reverse
start: 5880
end: 6796
orientation: inline

BBa_J24671
type: SequenceAnnotation
persistent identity: annotation2324215
display id: annotation2324215
version: 1
top level: BBa_J24672
location: https://synbiohub.org/public/igem/BBa_J24672/annotation2324215/range2324215/1
component: BBa_J24671

https://synbiohub.org/public/igem/BBa_J24672/annotation2324215/range2324215/1
type: Range
persistent identity: range2324215
display id: range2324215
version: 1
top level: BBa_J24672
direction: direction/reverse
start: 6805
end: 9003
orientation: inline

BBa_E0438
type: SequenceAnnotation
persistent identity: annotation2324229
display id: annotation2324229
version: 1
top level: BBa_J24672
location: https://synbiohub.org/public/igem/BBa_J24672/annotation2324229/range2324229/1
component: BBa_E0438

https://synbiohub.org/public/igem/BBa_J24672/annotation2324229/range2324229/1
type: Range
persistent identity: range2324229
display id: range2324229
version: 1
top level: BBa_J24672
direction: direction/reverse
start: 9012
end: 9894
orientation: inline

BBa_E0438
type: ComponentDefinition
persistent identity: BBa E0438
display id: BBa_E0438
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.YFP LVA+.Term
created: 2005-06-29T11:00:00Z
modified: 2015-08-31T04:07:26Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 6
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_E0032
component: BBa_B0034
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_E0032
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_E0438_sequence/1

BBa_E0032
type: Component
persistent identity: component1557979
display id: component1557979
version: 1
top level: BBa_E0438
definition: YFP
access: public

BBa_B0034
type: Component
persistent identity: component1557967
display id: component1557967
version: 1
top level: BBa_E0438
definition: BBa_B0034
access: public

BBa_B0011
type: Component
persistent identity: component1557999
display id: component1557999
version: 1
top level: BBa_E0438
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component1557991
display id: component1557991
version: 1
top level: BBa_E0438
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1557967
display id: annotation1557967
version: 1
top level: BBa_E0438
location: https://synbiohub.org/public/igem/BBa_E0438/annotation1557967/range1557967/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_E0438/annotation1557967/range1557967/1
type: Range
persistent identity: range1557967
display id: range1557967
version: 1
top level: BBa_E0438
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation1557979
display id: annotation1557979
version: 1
top level: BBa_E0438
location: https://synbiohub.org/public/igem/BBa_E0438/annotation1557979/range1557979/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_E0438/annotation1557979/range1557979/1
type: Range
persistent identity: range1557979
display id: range1557979
version: 1
top level: BBa_E0438
direction: direction/forward
start: 19
end: 780
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1557991
display id: annotation1557991
version: 1
top level: BBa_E0438
location: https://synbiohub.org/public/igem/BBa_E0438/annotation1557991/range1557991/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0438/annotation1557991/range1557991/1
type: Range
persistent identity: range1557991
display id: range1557991
version: 1
top level: BBa_E0438
direction: direction/forward
start: 789
end: 829
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation1557999
display id: annotation1557999
version: 1
top level: BBa_E0438
location: https://synbiohub.org/public/igem/BBa_E0438/annotation1557999/range1557999/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_E0438/annotation1557999/range1557999/1
type: Range
persistent identity: range1557999
display id: range1557999
version: 1
top level: BBa_E0438
direction: direction/forward
start: 838
end: 883
orientation: inline

mCherry
type: ComponentDefinition
persistent identity: BBa J06703
display id: BBa_J06703
version: 1
was generated by: iGEM to SBOL conversion
description: mCherry, bacterial (LVA+) with RBS and forward terminator
created: 2005-07-24T11:00:00Z
modified: 2015-08-31T04:08:18Z
owned by: james
owned by: myers
mutable description: Combines BBa_B0015 forward terminator with BBa_J06603 mCherry, bacterial (LVA+) with RBS
discontinued: false
dominant: true
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Yves Wang
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0012
component: BBa_B0010
component: BBa_J06505
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_J06505
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J06703_sequence/1

BBa_B0012
type: Component
persistent identity: component1596489
display id: component1596489
version: 1
top level: mCherry
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1596479
display id: component1596479
version: 1
top level: mCherry
definition: BBa_B0010
access: public

BBa_J06505
type: Component
persistent identity: component1596474
display id: component1596474
version: 1
top level: mCherry
definition: mCherry
access: public

BBa_B0034
type: Component
persistent identity: component1596463
display id: component1596463
version: 1
top level: mCherry
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1596463
display id: annotation1596463
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06703/annotation1596463/range1596463/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06703/annotation1596463/range1596463/1
type: Range
persistent identity: range1596463
display id: range1596463
version: 1
top level: mCherry
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_J06505
type: SequenceAnnotation
persistent identity: annotation1596474
display id: annotation1596474
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06703/annotation1596474/range1596474/1
component: BBa_J06505

https://synbiohub.org/public/igem/BBa_J06703/annotation1596474/range1596474/1
type: Range
persistent identity: range1596474
display id: range1596474
version: 1
top level: mCherry
direction: direction/forward
start: 19
end: 741
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1596479
display id: annotation1596479
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06703/annotation1596479/range1596479/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J06703/annotation1596479/range1596479/1
type: Range
persistent identity: range1596479
display id: range1596479
version: 1
top level: mCherry
direction: direction/forward
start: 750
end: 829
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1596489
display id: annotation1596489
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06703/annotation1596489/range1596489/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J06703/annotation1596489/range1596489/1
type: Range
persistent identity: range1596489
display id: range1596489
version: 1
top level: mCherry
direction: direction/forward
start: 838
end: 878
orientation: inline

BBa_E0422
type: ComponentDefinition
persistent identity: BBa E0422
display id: BBa_E0422
version: 1
was generated by: iGEM to SBOL conversion
description: ECFP (RBS+ LVA+ TERM) (B0034.E0022.B0015)
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:26Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0034
component: BBa_B0010
component: BBa_E0022
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_E0022
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_E0422_sequence/1

BBa_B0034
type: Component
persistent identity: component942803
display id: component942803
version: 1
top level: BBa_E0422
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component942823
display id: component942823
version: 1
top level: BBa_E0422
definition: BBa_B0010
access: public

BBa_E0022
type: Component
persistent identity: component942815
display id: component942815
version: 1
top level: BBa_E0422
definition: ECFP
access: public

BBa_B0012
type: Component
persistent identity: component942833
display id: component942833
version: 1
top level: BBa_E0422
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation942803
display id: annotation942803
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942803/range942803/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_E0422/annotation942803/range942803/1
type: Range
persistent identity: range942803
display id: range942803
version: 1
top level: BBa_E0422
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation942815
display id: annotation942815
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942815/range942815/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_E0422/annotation942815/range942815/1
type: Range
persistent identity: range942815
display id: range942815
version: 1
top level: BBa_E0422
direction: direction/forward
start: 19
end: 780
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation942823
display id: annotation942823
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942823/range942823/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0422/annotation942823/range942823/1
type: Range
persistent identity: range942823
display id: range942823
version: 1
top level: BBa_E0422
direction: direction/forward
start: 789
end: 868
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation942833
display id: annotation942833
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942833/range942833/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0422/annotation942833/range942833/1
type: Range
persistent identity: range942833
display id: range942833
version: 1
top level: BBa_E0422
direction: direction/forward
start: 877
end: 917
orientation: inline

BBa_J24669
type: ComponentDefinition
persistent identity: BBa J24669
display id: BBa_J24669
version: 1
was generated by: iGEM to SBOL conversion
description: Tri-Stable Toggle (Arabinose induced component)
created: 2006-07-23T11:00:00Z
modified: 2015-08-31T04:08:41Z
mutable provenance: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaa tagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgc gccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaatt gctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagt aacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggct ggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactg gtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccc tgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcct caatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccatt cagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaa agcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcg tcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgttttt ttgggctagcaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaaccc gtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttaga taggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgc gatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactag agaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacc tactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcata tgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagat cacaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccgg…
owned by: james
owned by: myers
mutable description: This component of the tri-stable toggle switch is activated by arabinose and inhibits pTetR and pLacI by producing both TetR and LacI LVA-tagged proteins. This component is repressed by AraC. It is unterminated to allow for the addition of target genes.
discontinued: false
dominant: false
experience: experience/None
group u list: _58_
m user id: 0
owner id: 1061
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: None
star: false
creator: Azeem Kaka
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_I0500
component: BBa_S03520
component: BBa_S03518
sequence annotation: BBa_I0500
sequence annotation: BBa_S03518
sequence annotation: BBa_S03520
sequence: https://synbiohub.org/public/igem/BBa_J24669_sequence/1

BBa_I0500
type: Component
persistent identity: component2251630
display id: component2251630
version: 1
top level: BBa_J24669
definition: pBad/araC
access: public

BBa_S03520
type: Component
persistent identity: component2251644
display id: component2251644
version: 1
top level: BBa_J24669
definition: BBa_S03520
access: public

BBa_S03518
type: Component
persistent identity: component2251637
display id: component2251637
version: 1
top level: BBa_J24669
definition: BBa_S03518
access: public

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2251630
display id: annotation2251630
version: 1
top level: BBa_J24669
location: https://synbiohub.org/public/igem/BBa_J24669/annotation2251630/range2251630/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_J24669/annotation2251630/range2251630/1
type: Range
persistent identity: range2251630
display id: range2251630
version: 1
top level: BBa_J24669
direction: direction/reverse
start: 1
end: 1210
orientation: inline

BBa_S03518
type: SequenceAnnotation
persistent identity: annotation2251637
display id: annotation2251637
version: 1
top level: BBa_J24669
location: https://synbiohub.org/public/igem/BBa_J24669/annotation2251637/range2251637/1
component: BBa_S03518

https://synbiohub.org/public/igem/BBa_J24669/annotation2251637/range2251637/1
type: Range
persistent identity: range2251637
display id: range2251637
version: 1
top level: BBa_J24669
direction: direction/reverse
start: 1219
end: 1921
orientation: inline

BBa_S03520
type: SequenceAnnotation
persistent identity: annotation2251644
display id: annotation2251644
version: 1
top level: BBa_J24669
location: https://synbiohub.org/public/igem/BBa_J24669/annotation2251644/range2251644/1
component: BBa_S03520

https://synbiohub.org/public/igem/BBa_J24669/annotation2251644/range2251644/1
type: Range
persistent identity: range2251644
display id: range2251644
version: 1
top level: BBa_J24669
direction: direction/reverse
start: 1930
end: 3100
orientation: inline

BBa_J24670
type: ComponentDefinition
persistent identity: BBa J24670
display id: BBa_J24670
version: 1
was generated by: iGEM to SBOL conversion
description: Tri-Stable Toggle (Lactose induced component)
created: 2006-07-23T11:00:00Z
modified: 2015-08-31T04:08:41Z
mutable provenance: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaat taatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaaccc gtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttaga taggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgc gatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactag agaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacc tactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcata tgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagat cacaaagaggagaaatactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgagg ccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcaggg acgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccag tgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagc cgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttact gcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaat tttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcg aggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctattt ctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctgca aacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
owned by: james
owned by: myers
mutable description: This component of the tri-stable toggle switch is activated by lactose and inhibits pTetR and pAraBad by producing both TetR and AraBad LVA-tagged proteins. This component is repressed by LacI. It is unterminated to allow for the addition of target genes.
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 1061
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Azeem Kaka
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_S03519
component: BBa_S03518
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_S03518
sequence annotation: BBa_S03519
sequence: https://synbiohub.org/public/igem/BBa_J24670_sequence/1

BBa_S03519
type: Component
persistent identity: component2251665
display id: component2251665
version: 1
top level: BBa_J24670
definition: BBa_S03519
access: public

BBa_S03518
type: Component
persistent identity: component2251658
display id: component2251658
version: 1
top level: BBa_J24670
definition: BBa_S03518
access: public

BBa_R0010
type: Component
persistent identity: component2251645
display id: component2251645
version: 1
top level: BBa_J24670
definition: LacI
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2251645
display id: annotation2251645
version: 1
top level: BBa_J24670
location: https://synbiohub.org/public/igem/BBa_J24670/annotation2251645/range2251645/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J24670/annotation2251645/range2251645/1
type: Range
persistent identity: range2251645
display id: range2251645
version: 1
top level: BBa_J24670
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_S03518
type: SequenceAnnotation
persistent identity: annotation2251658
display id: annotation2251658
version: 1
top level: BBa_J24670
location: https://synbiohub.org/public/igem/BBa_J24670/annotation2251658/range2251658/1
component: BBa_S03518

https://synbiohub.org/public/igem/BBa_J24670/annotation2251658/range2251658/1
type: Range
persistent identity: range2251658
display id: range2251658
version: 1
top level: BBa_J24670
direction: direction/reverse
start: 209
end: 911
orientation: inline

BBa_S03519
type: SequenceAnnotation
persistent identity: annotation2251665
display id: annotation2251665
version: 1
top level: BBa_J24670
location: https://synbiohub.org/public/igem/BBa_J24670/annotation2251665/range2251665/1
component: BBa_S03519

https://synbiohub.org/public/igem/BBa_J24670/annotation2251665/range2251665/1
type: Range
persistent identity: range2251665
display id: range2251665
version: 1
top level: BBa_J24670
direction: direction/reverse
start: 920
end: 1877
orientation: inline

BBa_J24671
type: ComponentDefinition
persistent identity: BBa J24671
display id: BBa_J24671
version: 1
was generated by: iGEM to SBOL conversion
description: Tri-Stable Toggle (Tetracycline induced component)
created: 2006-07-23T11:00:00Z
modified: 2015-08-31T04:08:41Z
mutable provenance: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtga accaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcggg caaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggt gccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcatta actatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacag tattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttct gtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccg gttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattac cgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacag gattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtga aaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaag cgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctcaaagaggagaaatactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgagg ccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcaggg acgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccag tgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagc cgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttact gcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaat tttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcg aggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcg…
owned by: james
owned by: myers
mutable description: This component of the tri-stable toggle switch is activated by tetracycline and inhibits pLac and pAraBad by producing both LacI and AraBad LVA-tagged proteins. This component is repressed by TetR. It is unterminated to allow for the addition of target genes.
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 1061
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Azeem Kaka
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
component: BBa_S03519
component: BBa_S03520
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_S03520
sequence annotation: BBa_S03519
sequence: https://synbiohub.org/public/igem/BBa_J24671_sequence/1

BBa_S03519
type: Component
persistent identity: component2251684
display id: component2251684
version: 1
top level: BBa_J24671
definition: BBa_S03519
access: public

BBa_S03520
type: Component
persistent identity: component2251677
display id: component2251677
version: 1
top level: BBa_J24671
definition: BBa_S03520
access: public

BBa_R0040
type: Component
persistent identity: component2251666
display id: component2251666
version: 1
top level: BBa_J24671
definition: p(tetR)
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2251666
display id: annotation2251666
version: 1
top level: BBa_J24671
location: https://synbiohub.org/public/igem/BBa_J24671/annotation2251666/range2251666/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J24671/annotation2251666/range2251666/1
type: Range
persistent identity: range2251666
display id: range2251666
version: 1
top level: BBa_J24671
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_S03520
type: SequenceAnnotation
persistent identity: annotation2251677
display id: annotation2251677
version: 1
top level: BBa_J24671
location: https://synbiohub.org/public/igem/BBa_J24671/annotation2251677/range2251677/1
component: BBa_S03520

https://synbiohub.org/public/igem/BBa_J24671/annotation2251677/range2251677/1
type: Range
persistent identity: range2251677
display id: range2251677
version: 1
top level: BBa_J24671
direction: direction/reverse
start: 63
end: 1233
orientation: inline

BBa_S03519
type: SequenceAnnotation
persistent identity: annotation2251684
display id: annotation2251684
version: 1
top level: BBa_J24671
location: https://synbiohub.org/public/igem/BBa_J24671/annotation2251684/range2251684/1
component: BBa_S03519

https://synbiohub.org/public/igem/BBa_J24671/annotation2251684/range2251684/1
type: Range
persistent identity: range2251684
display id: range2251684
version: 1
top level: BBa_J24671
direction: direction/reverse
start: 1242
end: 2199
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

YFP
type: ComponentDefinition
persistent identity: BBa E0032
display id: BBa_E0032
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced yellow fluorescent protein derived from A. victoria GFP
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modified from <bb_part>BBa_E0031</bb_part>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Yellow fluorescent protein (EYFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. Annotated mutation cause a Q81L mutation that appears to not be near the active site.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_E0032 yellow fluorescent protein is based on BioBrick part BBa_E0031. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: YFP (LVA)
sequence annotation: BBa_E0032
sequence annotation: A (Q->L)
sequence annotation: SsrA
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_E0032_sequence/1

YFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2156
display id: annotation2156
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1
type: Range
persistent identity: range2156
display id: range2156
version: 1
top level: YFP
direction: direction/forward
start: 1
end: 762
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation7041
display id: annotation7041
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1
type: Range
persistent identity: range7041
display id: range7041
version: 1
top level: YFP
direction: direction/forward
start: 1
end: 762
orientation: inline

A (Q->L)
type: SequenceAnnotation
persistent identity: annotation2161
display id: annotation2161
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1
type: Range
persistent identity: range2161
display id: range2161
version: 1
top level: YFP
direction: direction/forward
start: 242
end: 242
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2160
display id: annotation2160
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1
type: Range
persistent identity: range2160
display id: range2160
version: 1
top level: YFP
direction: direction/forward
start: 718
end: 756
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2159
display id: annotation2159
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1
type: Range
persistent identity: range2159
display id: range2159
version: 1
top level: YFP
direction: direction/forward
start: 757
end: 762
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

pBad/araC
type: ComponentDefinition
persistent identity: BBa I0500
display id: BBa_I0500
version: 1
was generated by: iGEM to SBOL conversion
description: Inducible pBad/araC promoter
created: 2003-12-05T12:00:00Z
modified: 2015-08-31T04:07:29Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Sri Kosuri
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: AraC
sequence annotation: PC
sequence annotation: Pbad
sequence: https://synbiohub.org/public/igem/BBa_I0500_sequence/1

AraC
type: SequenceAnnotation
persistent identity: annotation1498843
display id: annotation1498843
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
type: Range
persistent identity: range1498843
display id: range1498843
version: 1
top level: pBad/araC
direction: direction/forward
start: 1
end: 1079
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation1498842
display id: annotation1498842
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
type: Range
persistent identity: range1498842
display id: range1498842
version: 1
top level: pBad/araC
direction: direction/forward
start: 1043
end: 1114
orientation: inline

Pbad
type: SequenceAnnotation
persistent identity: annotation1498841
display id: annotation1498841
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
type: Range
persistent identity: range1498841
display id: range1498841
version: 1
top level: pBad/araC
direction: direction/forward
start: 1080
end: 1210
orientation: inline

ECFP
type: ComponentDefinition
persistent identity: BBa E0022
display id: BBa_E0022
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced cyan fluorescent protein derived from A. victoria GFP
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modified from <bb_part>BBa_E0021</bb_part>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_E0022 cyan fluorescent protein is based on BioBrick part BBa_E0021. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: BBa_E0022
sequence annotation: CFP (LVA)
sequence annotation: A
sequence annotation: SsrA
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_E0022_sequence/1

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation7040
display id: annotation7040
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
type: Range
persistent identity: range7040
display id: range7040
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

CFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2150
display id: annotation2150
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
type: Range
persistent identity: range2150
display id: range2150
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation2155
display id: annotation2155
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
type: Range
persistent identity: range2155
display id: range2155
version: 1
top level: ECFP
direction: direction/forward
start: 69
end: 69
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2153
display id: annotation2153
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
type: Range
persistent identity: range2153
display id: range2153
version: 1
top level: ECFP
direction: direction/forward
start: 719
end: 756
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2154
display id: annotation2154
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
type: Range
persistent identity: range2154
display id: range2154
version: 1
top level: ECFP
direction: direction/forward
start: 757
end: 762
orientation: inline

BBa_S03519
type: ComponentDefinition
persistent identity: BBa S03519
display id: BBa_S03519
version: 1
was generated by: iGEM to SBOL conversion
description: B0034:C0080
created: 2006-07-23T11:00:00Z
modified: 2015-05-08T01:14:24Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/None
group u list: _58_
m user id: 0
owner id: 1061
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
star: false
creator: Azeem Kaka
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_C0080
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0080
sequence: https://synbiohub.org/public/igem/BBa_S03519_sequence/1

BBa_C0080
type: Component
persistent identity: component2243691
display id: component2243691
version: 1
top level: BBa_S03519
definition: araC
access: public

BBa_B0034
type: Component
persistent identity: component2243687
display id: component2243687
version: 1
top level: BBa_S03519
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2243687
display id: annotation2243687
version: 1
top level: BBa_S03519
location: https://synbiohub.org/public/igem/BBa_S03519/annotation2243687/range2243687/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03519/annotation2243687/range2243687/1
type: Range
persistent identity: range2243687
display id: range2243687
version: 1
top level: BBa_S03519
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_C0080
type: SequenceAnnotation
persistent identity: annotation2243691
display id: annotation2243691
version: 1
top level: BBa_S03519
location: https://synbiohub.org/public/igem/BBa_S03519/annotation2243691/range2243691/1
component: BBa_C0080

https://synbiohub.org/public/igem/BBa_S03519/annotation2243691/range2243691/1
type: Range
persistent identity: range2243691
display id: range2243691
version: 1
top level: BBa_S03519
direction: direction/reverse
start: 19
end: 958
orientation: inline

BBa_S03520
type: ComponentDefinition
persistent identity: BBa S03520
display id: BBa_S03520
version: 1
was generated by: iGEM to SBOL conversion
description: B0034:C0012
created: 2006-07-23T11:00:00Z
modified: 2015-05-08T01:14:24Z
owned by: james
owned by: myers
part status: Released HQ 2013
discontinued: false
dominant: false
experience: experience/None
group u list: _58_
m user id: 0
owner id: 1061
owning group id: 58
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: Azeem Kaka
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_C0012
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0012
sequence: https://synbiohub.org/public/igem/BBa_S03520_sequence/1

BBa_C0012
type: Component
persistent identity: component2243695
display id: component2243695
version: 1
top level: BBa_S03520
definition: lacI
access: public

BBa_B0034
type: Component
persistent identity: component2243693
display id: component2243693
version: 1
top level: BBa_S03520
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2243693
display id: annotation2243693
version: 1
top level: BBa_S03520
location: https://synbiohub.org/public/igem/BBa_S03520/annotation2243693/range2243693/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03520/annotation2243693/range2243693/1
type: Range
persistent identity: range2243693
display id: range2243693
version: 1
top level: BBa_S03520
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2243695
display id: annotation2243695
version: 1
top level: BBa_S03520
location: https://synbiohub.org/public/igem/BBa_S03520/annotation2243695/range2243695/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_S03520/annotation2243695/range2243695/1
type: Range
persistent identity: range2243695
display id: range2243695
version: 1
top level: BBa_S03520
direction: direction/forward
start: 19
end: 1146
orientation: inline

BBa_S03518
type: ComponentDefinition
persistent identity: BBa S03518
display id: BBa_S03518
version: 1
was generated by: iGEM to SBOL conversion
description: B0034:C0040
created: 2006-07-23T11:00:00Z
modified: 2015-05-08T01:14:24Z
owned by: james
owned by: myers
part status: Released HQ 2013
discontinued: false
dominant: false
experience: experience/None
group u list: _58_
m user id: 0
owner id: 1061
owning group id: 58
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: Azeem Kaka
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_C0040
sequence annotation: BBa_B0034
sequence annotation: BBa_C0040
sequence: https://synbiohub.org/public/igem/BBa_S03518_sequence/1

BBa_B0034
type: Component
persistent identity: component2243681
display id: component2243681
version: 1
top level: BBa_S03518
definition: BBa_B0034
access: public

BBa_C0040
type: Component
persistent identity: component2243685
display id: component2243685
version: 1
top level: BBa_S03518
definition: tetR
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2243681
display id: annotation2243681
version: 1
top level: BBa_S03518
location: https://synbiohub.org/public/igem/BBa_S03518/annotation2243681/range2243681/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03518/annotation2243681/range2243681/1
type: Range
persistent identity: range2243681
display id: range2243681
version: 1
top level: BBa_S03518
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2243685
display id: annotation2243685
version: 1
top level: BBa_S03518
location: https://synbiohub.org/public/igem/BBa_S03518/annotation2243685/range2243685/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_S03518/annotation2243685/range2243685/1
type: Range
persistent identity: range2243685
display id: range2243685
version: 1
top level: BBa_S03518
direction: direction/reverse
start: 19
end: 703
orientation: inline

mCherry
type: ComponentDefinition
persistent identity: BBa J06505
display id: BBa_J06505
version: 1
was generated by: iGEM to SBOL conversion
description: monomeric RFP optimized for bacteria
created: 2005-07-17T11:00:00Z
modified: 2015-08-31T04:08:18Z
mutable provenance: mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible and with an LVA tag added.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: mRFP (DsRed) derived, altered to be a biobrick by removing a PstI site and adding in the ends.
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends with the LVA tag using PCR. Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick.
star: false
creator: ytwang
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: mCherry
sequence annotation: C->T (removing PstI site)
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_J06505_sequence/1

mCherry
type: SequenceAnnotation
persistent identity: annotation1585879
display id: annotation1585879
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06505/annotation1585879/range1585879/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J06505/annotation1585879/range1585879/1
type: Range
persistent identity: range1585879
display id: range1585879
version: 1
top level: mCherry
direction: direction/forward
start: 1
end: 708
orientation: inline

C->T (removing PstI site)
type: SequenceAnnotation
persistent identity: annotation1585880
display id: annotation1585880
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06505/annotation1585880/range1585880/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_J06505/annotation1585880/range1585880/1
type: Range
persistent identity: range1585880
display id: range1585880
version: 1
top level: mCherry
direction: direction/forward
start: 352
end: 352
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1585887
display id: annotation1585887
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06505/annotation1585887/range1585887/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_J06505/annotation1585887/range1585887/1
type: Range
persistent identity: range1585887
display id: range1585887
version: 1
top level: mCherry
direction: direction/forward
start: 709
end: 717
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

araC
type: ComponentDefinition
persistent identity: BBa C0080
display id: BBa_C0080
version: 1
was generated by: iGEM to SBOL conversion
description: araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
mutable provenance: GenBank: NC_002655 (www.ncbi.nlm.nih.gov)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: coding region for the araC gene, used to make araC protein for use in positive or negative regulation of TIPs output (see also R0080, R0081)
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Sara Neves (Fighting Darwins)
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: araC
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0080_sequence/1

araC
type: SequenceAnnotation
persistent identity: annotation308191
display id: annotation308191
version: 1
top level: araC
location: https://synbiohub.org/public/igem/BBa_C0080/annotation308191/range308191/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0080/annotation308191/range308191/1
type: Range
persistent identity: range308191
display id: range308191
version: 1
top level: araC
direction: direction/forward
start: 1
end: 876
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation308196
display id: annotation308196
version: 1
top level: araC
location: https://synbiohub.org/public/igem/BBa_C0080/annotation308196/range308196/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0080/annotation308196/range308196/1
type: Range
persistent identity: range308196
display id: range308196
version: 1
top level: araC
direction: direction/forward
start: 877
end: 909
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214004
display id: annotation2214004
version: 1
top level: araC
location: https://synbiohub.org/public/igem/BBa_C0080/annotation2214004/range2214004/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0080/annotation2214004/range2214004/1
type: Range
persistent identity: range2214004
display id: range2214004
version: 1
top level: araC
direction: direction/forward
start: 916
end: 940
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa C0012
display id: BBa_C0012
version: 1
was generated by: iGEM to SBOL conversion
description: lacI repressor from E. coli (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG.
star: true
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0012
sequence annotation: lacI-LVA
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0012_sequence/1

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation7031
display id: annotation7031
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
type: Range
persistent identity: range7031
display id: range7031
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

lacI-LVA
type: SequenceAnnotation
persistent identity: annotation1723
display id: annotation1723
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
type: Range
persistent identity: range1723
display id: range1723
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1722
display id: annotation1722
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
type: Range
persistent identity: range1722
display id: range1722
version: 1
top level: lacI
direction: direction/forward
start: 1090
end: 1128
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213988
display id: annotation2213988
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
type: Range
persistent identity: range2213988
display id: range2213988
version: 1
top level: lacI
direction: direction/forward
start: 1129
end: 1153
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

https://synbiohub.org/public/igem/BBa_J24672_sequence/1
type: Sequence
persistent identity: BBa J24672 sequence
display id: BBa_J24672_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0438_sequence/1
type: Sequence
persistent identity: BBa E0438 sequence
display id: BBa_E0438_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J06703_sequence/1
type: Sequence
persistent identity: BBa J06703 sequence
display id: BBa_J06703_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0422_sequence/1
type: Sequence
persistent identity: BBa E0422 sequence
display id: BBa_E0422_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J24669_sequence/1
type: Sequence
persistent identity: BBa J24669 sequence
display id: BBa_J24669_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J24670_sequence/1
type: Sequence
persistent identity: BBa J24670 sequence
display id: BBa_J24670_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J24671_sequence/1
type: Sequence
persistent identity: BBa J24671 sequence
display id: BBa_J24671_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgcc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0032_sequence/1
type: Sequence
persistent identity: BBa E0032 sequence
display id: BBa_E0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I0500_sequence/1
type: Sequence
persistent identity: BBa I0500 sequence
display id: BBa_I0500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0022_sequence/1
type: Sequence
persistent identity: BBa E0022 sequence
display id: BBa_E0022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S03519_sequence/1
type: Sequence
persistent identity: BBa S03519 sequence
display id: BBa_S03519_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S03520_sequence/1
type: Sequence
persistent identity: BBa S03520 sequence
display id: BBa_S03520_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S03518_sequence/1
type: Sequence
persistent identity: BBa S03518 sequence
display id: BBa_S03518_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J06505_sequence/1
type: Sequence
persistent identity: BBa J06505 sequence
display id: BBa_J06505_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0080_sequence/1
type: Sequence
persistent identity: BBa C0080 sequence
display id: BBa_C0080_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0012_sequence/1
type: Sequence
persistent identity: BBa C0012 sequence
display id: BBa_C0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z