##gff-version 3
##sequence-region BBa_J24676 1 3977
BBa_J24676	.	engineered_region	1930	3100	.	+	0	ID=BBa_S03520;Name=BBa_S03520
BBa_J24676	.	ribosome_entry_site	1930	1941	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03520
BBa_J24676	.	conserved_region	1934	1937	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24676	.	CDS	1948	3075	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_S03520
BBa_J24676	.	engineered_tag	3076	3100	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J24676	.	engineered_region	1948	3075	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J24676	.	tag	3037	3075	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J24676	.	CDS	1948	3075	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J24676	.	engineered_region	1219	1921	.	+	0	ID=BBa_S03518;Name=BBa_S03518
BBa_J24676	.	CDS	1237	1921	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S03518
BBa_J24676	.	engineered_tag	1897	1921	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J24676	.	CDS	1240	1856	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J24676	.	tag	1857	1890	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J24676	.	ribosome_entry_site	1219	1230	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03518
BBa_J24676	.	conserved_region	1223	1226	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24676	.	engineered_region	3109	3977	.	+	0	ID=BBa_J06702;Name=BBa_J06702
BBa_J24676	.	terminator	3937	3977	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J06702
BBa_J24676	.	stop_codon	3970	3970	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J24676	.	polyA_site	3964	3977	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24676	.	stem_loop	3944	3963	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J24676	.	engineered_region	3937	3977	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24676	.	terminator	3849	3928	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J06702
BBa_J24676	.	engineered_region	3849	3928	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J24676	.	stem_loop	3860	3903	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24676	.	ribosome_entry_site	3109	3120	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J06702
BBa_J24676	.	conserved_region	3113	3116	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24676	.	engineered_region	3127	3840	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_J06702
BBa_J24676	.	sequence_alteration	3478	3478	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_J24676	.	CDS	3127	3837	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_J24676	.	promoter	1	1210	.	+	0	ID=BBa_I0500;Name=BBa_I0500
BBa_J24676	.	promoter	1043	1114	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_J24676	.	sequence_feature	1	1079	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_J24676	.	promoter	1080	1210	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
>BBa_J24676
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat
tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg
tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc
tccatacccgtttttttgggctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtga
ttaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgt
agagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagatagg
caccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtg
ctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaa
tcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttact
ttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgc
cattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcat
atgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataa
cactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttat
acgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaa
aacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaa
cagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctc
gcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgca
caatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagct
gcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaag
acggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttc
tgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaa
ggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctgg
ttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggt
agtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctg
gggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcac
tggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagct
ggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctga
tagtgctagtgtagatctctactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggcca
tcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgaggg
cgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggac
atcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgt
ccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctc
cttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaag
aagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggc
tgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccgg
cgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgcc
gagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttata