LOCUS       BBa_J24705              1591 bp    DNA     linear       10-May-2024
DEFINITION  25. Weak RBS - TetR - Strong RBS - mCherry(+LVA) - terminator
ACCESSION   BBa_J24705
VERSION     BBa_J24705.1  
FEATURES             Location/Qualifiers
     misc_feature    7..10
                     /label=conserved
     RBS             1..14
                     /label=BBa_B0031
     CDS             24..640
                     /label=tetR
     misc_feature    641..674
                     /label=SsrA
     misc_feature    681..705
                     /label=Help:Barcodes
     CDS             21..705
                     /label=BBa_C0040
     misc_feature    1..705
                     /label=BBa_J24686
     misc_feature    718..721
                     /label=conserved
     RBS             714..725
                     /label=BBa_B0034
     CDS             732..1439
                     /label=mCherry
     misc_feature    1083..1083
                     /label=C->T (removing PstI site)
     misc_feature    1440..1448
                     /label=LVA
     misc_feature    732..1454
                     /label=BBa_J06505
     stem_loop       1474..1517
                     /label=stem_loop
     misc_feature    1463..1542
                     /label=BBa_B0010
     terminator      1463..1542
                     /label=BBa_B0010
     stem_loop       1558..1577
                     /label=T7 TE
     misc_feature    1584..1584
                     /label=stop
     polyA_site      1578..1591
                     /label=polya
     misc_feature    1551..1591
                     /label=BBa_B0012
     terminator      1551..1591
                     /label=BBa_B0012
     misc_feature    714..1591
                     /label=BBa_J24702
ORIGIN
        1 tcacacagga aacctactag atgtccagat tagataaaag taaagtgatt aacagcgcat
       61 tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc
      121 taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg
      181 ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa
      241 gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc
      301 gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg
      361 aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac
      421 tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg
      481 ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta
      541 tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca
      601 tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact
      661 acgctttagt agcttaataa cactgatagt gctagtgtag atcactacta gagaaagagg
      721 agaaatacta gatggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
      781 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
      841 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
      901 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
      961 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
     1021 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
     1081 ccttgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
     1141 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc
     1201 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
     1261 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
     1321 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
     1381 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt
     1441 tagtagctta ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     1501 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     1561 tcaccttcgg gtgggccttt ctgcgtttat a
//