##gff-version 3
##sequence-region BBa_J24705 1 1591
BBa_J24705	.	engineered_region	714	1591	.	+	0	ID=BBa_J24702;Name=BBa_J24702
BBa_J24705	.	engineered_region	732	1454	.	+	0	ID=BBa_J06505;Name=BBa_J06505;Parent=BBa_J24702
BBa_J24705	.	CDS	732	1439	.	+	0	ID=annotation1585879;Name=mCherry;Parent=BBa_J06505
BBa_J24705	.	tag	1440	1448	.	+	0	ID=annotation1585887;Name=LVA;Parent=BBa_J06505
BBa_J24705	.	sequence_alteration	1083	1083	.	+	0	ID=annotation1585880;Name=C->T (removing PstI site);Parent=BBa_J06505
BBa_J24705	.	terminator	1463	1542	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J24702
BBa_J24705	.	stem_loop	1474	1517	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24705	.	engineered_region	1463	1542	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J24705	.	terminator	1551	1591	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J24702
BBa_J24705	.	engineered_region	1551	1591	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24705	.	stop_codon	1584	1584	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J24705	.	stem_loop	1558	1577	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J24705	.	polyA_site	1578	1591	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24705	.	ribosome_entry_site	714	725	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24702
BBa_J24705	.	conserved_region	718	721	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24705	.	engineered_region	1	705	.	+	0	ID=BBa_J24686;Name=BBa_J24686
BBa_J24705	.	CDS	21	705	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_J24686
BBa_J24705	.	engineered_tag	681	705	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J24705	.	tag	641	674	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J24705	.	CDS	24	640	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J24705	.	ribosome_entry_site	1	14	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_J24686
BBa_J24705	.	conserved_region	7	10	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
>BBa_J24705
tcacacaggaaacctactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggt
cggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaa
aataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaagggg
aaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagt
acatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttt
tcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatc
aagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttga
tcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaa
agtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactag
agaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaa
ggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacc
cagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacg
gctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtggga
gcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctac
aaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcct
ccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccacta
cgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttg
gacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggca
tggacgagctgtacaagttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagact
gggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggccttt
ctgcgtttata