LOCUS       BBa_J24706              1590 bp    DNA     linear       25-Nov-2024
DEFINITION  26. M RBS-TetR-S RBS-mCherry(+LVA)-terminator
ACCESSION   BBa_J24706
VERSION     BBa_J24706.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     CDS             23..639
                     /label=tetR
     misc_feature    640..673
                     /label=SsrA
     misc_feature    680..704
                     /label=Help:Barcodes
     CDS             20..704
                     /label=BBa_C0040
     misc_feature    1..704
                     /label=BBa_J24687
     misc_feature    717..720
                     /label=conserved
     RBS             713..724
                     /label=BBa_B0034
     CDS             731..1438
                     /label=mCherry
     misc_feature    1082..1082
                     /label=C->T (removing PstI site)
     misc_feature    1439..1447
                     /label=LVA
     misc_feature    731..1453
                     /label=BBa_J06505
     stem_loop       1473..1516
                     /label=stem_loop
     misc_feature    1462..1541
                     /label=BBa_B0010
     terminator      1462..1541
                     /label=BBa_B0010
     stem_loop       1557..1576
                     /label=T7 TE
     misc_feature    1583..1583
                     /label=stop
     polyA_site      1577..1590
                     /label=polya
     misc_feature    1550..1590
                     /label=BBa_B0012
     terminator      1550..1590
                     /label=BBa_B0012
     misc_feature    713..1590
                     /label=BBa_J24702
ORIGIN
        1 tcacacagga aagtactaga tgtccagatt agataaaagt aaagtgatta acagcgcatt
       61 agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct
      121 aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc
      181 cttagccatt gagatgttag ataggcacca tactcacttt tgccctttag aaggggaaag
      241 ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg
      301 cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg aaactctcga
      361 aaatcaatta gcctttttat gccaacaagg tttttcacta gagaatgcat tatatgcact
      421 cagcgctgtg gggcatttta ctttaggttg cgtattggaa gatcaagagc atcaagtcgc
      481 taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac gacaagctat
      541 cgaattattt gatcaccaag gtgcagagcc agccttctta ttcggccttg aattgatcat
      601 atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc gctgcaaacg acgaaaacta
      661 cgctttagta gcttaataac actgatagtg ctagtgtaga tcactactag agaaagagga
      721 gaaatactag atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
      781 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
      841 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
      901 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
      961 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     1021 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     1081 cttgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     1141 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     1201 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
     1261 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     1321 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     1381 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagtt
     1441 agtagcttaa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1501 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     1561 caccttcggg tgggcctttc tgcgtttata
//