LOCUS       BBa_J24707              1589 bp    DNA     linear       29-Nov-2024
DEFINITION  27. S RBS - TetR - S RBS - mCherry (+LVA) - terminator
ACCESSION   BBa_J24707
VERSION     BBa_J24707.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             22..638
                     /label=tetR
     misc_feature    639..672
                     /label=SsrA
     misc_feature    679..703
                     /label=Help:Barcodes
     CDS             19..703
                     /label=BBa_C0040
     misc_feature    1..703
                     /label=BBa_J24688
     misc_feature    716..719
                     /label=conserved
     RBS             712..723
                     /label=BBa_B0034
     CDS             730..1437
                     /label=mCherry
     misc_feature    1081..1081
                     /label=C->T (removing PstI site)
     misc_feature    1438..1446
                     /label=LVA
     misc_feature    730..1452
                     /label=BBa_J06505
     stem_loop       1472..1515
                     /label=stem_loop
     misc_feature    1461..1540
                     /label=BBa_B0010
     terminator      1461..1540
                     /label=BBa_B0010
     stem_loop       1556..1575
                     /label=T7 TE
     misc_feature    1582..1582
                     /label=stop
     polyA_site      1576..1589
                     /label=polya
     misc_feature    1549..1589
                     /label=BBa_B0012
     terminator      1549..1589
                     /label=BBa_B0012
     misc_feature    712..1589
                     /label=BBa_J24702
ORIGIN
        1 aaagaggaga aatactagat gtccagatta gataaaagta aagtgattaa cagcgcatta
       61 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta
      121 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc
      181 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc
      241 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc
      301 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa
      361 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc
      421 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct
      481 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc
      541 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata
      601 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac
      661 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gaaagaggag
      721 aaatactaga tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
      781 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
      841 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
      901 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
      961 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     1021 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     1081 ttgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     1141 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     1201 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     1261 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     1321 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     1381 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtta
     1441 gtagcttaat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
     1501 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     1561 accttcgggt gggcctttct gcgtttata
//