##gff-version 3
##sequence-region BBa_J24707 1 1589
BBa_J24707	.	engineered_region	712	1589	.	+	0	ID=BBa_J24702;Name=BBa_J24702
BBa_J24707	.	engineered_region	730	1452	.	+	0	ID=BBa_J06505;Name=BBa_J06505;Parent=BBa_J24702
BBa_J24707	.	tag	1438	1446	.	+	0	ID=annotation1585887;Name=LVA;Parent=BBa_J06505
BBa_J24707	.	CDS	730	1437	.	+	0	ID=annotation1585879;Name=mCherry;Parent=BBa_J06505
BBa_J24707	.	sequence_alteration	1081	1081	.	+	0	ID=annotation1585880;Name=C->T (removing PstI site);Parent=BBa_J06505
BBa_J24707	.	terminator	1461	1540	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J24702
BBa_J24707	.	engineered_region	1461	1540	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J24707	.	stem_loop	1472	1515	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24707	.	terminator	1549	1589	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J24702
BBa_J24707	.	stem_loop	1556	1575	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J24707	.	stop_codon	1582	1582	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J24707	.	polyA_site	1576	1589	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24707	.	engineered_region	1549	1589	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24707	.	ribosome_entry_site	712	723	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24702
BBa_J24707	.	conserved_region	716	719	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24707	.	engineered_region	1	703	.	+	0	ID=BBa_J24688;Name=BBa_J24688
BBa_J24707	.	CDS	19	703	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_J24688
BBa_J24707	.	engineered_tag	679	703	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J24707	.	CDS	22	638	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J24707	.	tag	639	672	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J24707	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24688
BBa_J24707	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_J24707
aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcg
gaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaa
taagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaa
agctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtac
atttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttc
actagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaa
gtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatc
accaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaag
tgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagag
aaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaagg
tgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcaccca
gaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggc
tccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagc
gcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaa
ggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctcc
gagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacg
acgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttgga
catcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatg
gacgagctgtacaagttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgg
gcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttct
gcgtttata