LOCUS       BBa_J24708              1630 bp    DNA     linear       22-Dec-2024
DEFINITION  28. W RBS - TetR - S RBS - ECFP(+LVA) - terminator
ACCESSION   BBa_J24708
VERSION     BBa_J24708.1  
FEATURES             Location/Qualifiers
     misc_feature    7..10
                     /label=conserved
     RBS             1..14
                     /label=BBa_B0031
     CDS             24..640
                     /label=tetR
     misc_feature    641..674
                     /label=SsrA
     misc_feature    681..705
                     /label=Help:Barcodes
     CDS             21..705
                     /label=BBa_C0040
     misc_feature    1..705
                     /label=BBa_J24686
     misc_feature    718..721
                     /label=conserved
     RBS             714..725
                     /label=BBa_B0034
     CDS             732..1493
                     /label=CFP (LVA)
     misc_feature    1450..1487
                     /label=SsrA
     misc_feature    1488..1493
                     /label=2
     misc_feature    800..800
                     /label=A
     misc_feature    732..1493
                     /label=BBa_E0022
     misc_feature    732..1493
                     /label=BBa_E0022
     stem_loop       1513..1556
                     /label=stem_loop
     misc_feature    1502..1581
                     /label=BBa_B0010
     terminator      1502..1581
                     /label=BBa_B0010
     stem_loop       1597..1616
                     /label=T7 TE
     misc_feature    1623..1623
                     /label=stop
     polyA_site      1617..1630
                     /label=polya
     misc_feature    1590..1630
                     /label=BBa_B0012
     terminator      1590..1630
                     /label=BBa_B0012
     misc_feature    714..1630
                     /label=BBa_J24703
ORIGIN
        1 tcacacagga aacctactag atgtccagat tagataaaag taaagtgatt aacagcgcat
       61 tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc
      121 taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg
      181 ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa
      241 gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc
      301 gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg
      361 aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac
      421 tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg
      481 ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta
      541 tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca
      601 tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact
      661 acgctttagt agcttaataa cactgatagt gctagtgtag atcactacta gagaaagagg
      721 agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
      781 tcgagctgga cggcgacgtg aacggccaca agttcagcgt gtccggcgag ggcgagggcg
      841 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
      901 cctggcccac cctcgtgacc accctgacct ggggcgtgca gtgcttcagc cgctaccccg
      961 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     1021 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     1081 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     1141 tcctggggca caagctggag tacaactaca tcagccacaa cgtctatatc accgccgaca
     1201 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gacggcagcg
     1261 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     1321 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     1381 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     1441 tgtacaagag gcctgctgca aacgacgaaa actacgcttt agtagcttaa taatactaga
     1501 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1561 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     1621 tgcgtttata
//