##gff-version 3
##sequence-region BBa_J24708 1 1630
BBa_J24708	.	engineered_region	1	705	.	+	0	ID=BBa_J24686;Name=BBa_J24686
BBa_J24708	.	ribosome_entry_site	1	14	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_J24686
BBa_J24708	.	conserved_region	7	10	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_J24708	.	CDS	21	705	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_J24686
BBa_J24708	.	tag	641	674	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J24708	.	engineered_tag	681	705	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J24708	.	CDS	24	640	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J24708	.	engineered_region	714	1630	.	+	0	ID=BBa_J24703;Name=BBa_J24703
BBa_J24708	.	ribosome_entry_site	714	725	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24703
BBa_J24708	.	conserved_region	718	721	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24708	.	engineered_region	732	1493	.	+	0	ID=BBa_E0022;Name=BBa_E0022;Parent=BBa_J24703
BBa_J24708	.	point_mutation	800	800	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_J24708	.	tag	1450	1487	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_J24708	.	CDS	732	1493	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_J24708	.	stop_codon	1488	1493	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_J24708	.	engineered_region	732	1493	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_J24708	.	terminator	1590	1630	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J24703
BBa_J24708	.	polyA_site	1617	1630	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24708	.	engineered_region	1590	1630	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24708	.	stem_loop	1597	1616	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J24708	.	stop_codon	1623	1623	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J24708	.	terminator	1502	1581	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J24703
BBa_J24708	.	stem_loop	1513	1556	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24708	.	engineered_region	1502	1581	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_J24708
tcacacaggaaacctactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggt
cggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaa
aataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaagggg
aaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagt
acatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttt
tcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatc
aagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttga
tcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaa
agtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactag
agaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctgga
cggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaag
ttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttca
gccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccat
cttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgag
ctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtct
atatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgca
gctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacc
cagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatca
ctctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata