##gff-version 3
##sequence-region BBa_J24709 1 1629
BBa_J24709	.	engineered_region	713	1629	.	+	0	ID=BBa_J24703;Name=BBa_J24703
BBa_J24709	.	ribosome_entry_site	713	724	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24703
BBa_J24709	.	conserved_region	717	720	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24709	.	engineered_region	731	1492	.	+	0	ID=BBa_E0022;Name=BBa_E0022;Parent=BBa_J24703
BBa_J24709	.	point_mutation	799	799	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_J24709	.	tag	1449	1486	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_J24709	.	CDS	731	1492	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_J24709	.	stop_codon	1487	1492	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_J24709	.	engineered_region	731	1492	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_J24709	.	terminator	1589	1629	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J24703
BBa_J24709	.	polyA_site	1616	1629	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24709	.	engineered_region	1589	1629	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24709	.	stem_loop	1596	1615	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J24709	.	stop_codon	1622	1622	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J24709	.	terminator	1501	1580	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J24703
BBa_J24709	.	stem_loop	1512	1555	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24709	.	engineered_region	1501	1580	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J24709	.	engineered_region	1	704	.	+	0	ID=BBa_J24687;Name=BBa_J24687
BBa_J24709	.	CDS	20	704	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_J24687
BBa_J24709	.	tag	640	673	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J24709	.	engineered_tag	680	704	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J24709	.	CDS	23	639	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J24709	.	ribosome_entry_site	1	13	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J24687
BBa_J24709	.	engineered_region	1	13	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J24709	.	ribosome_entry_site	1	13	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J24709	.	ribosome_entry_site	7	10	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
>BBa_J24709
tcacacaggaaagtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtc
ggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaa
ataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaagggga
aagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagta
catttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggttttt
cactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatca
agtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgat
caccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaa
gtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactaga
gaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggac
ggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagt
tcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcag
ccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatc
ttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagc
tgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtcta
tatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcag
ctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcaccc
agtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcac
tctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagc
caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
tactagagtcacactggctcaccttcgggtgggcctttctgcgtttata