LOCUS       BBa_J24711              2098 bp    DNA     linear       22-Dec-2024
DEFINITION  31. W RBS - LacI - S RBS - EYFP(+LVA) - terminator
ACCESSION   BBa_J24711
VERSION     BBa_J24711.1  
FEATURES             Location/Qualifiers
     misc_feature    7..10
                     /label=conserved
     RBS             1..14
                     /label=BBa_B0031
     misc_feature    1110..1148
                     /label=LVA
     CDS             21..1148
                     /label=lacI-LVA
     misc_feature    21..1148
                     /label=BBa_C0012
     misc_feature    1149..1173
                     /label=Help:Barcodes
     CDS             21..1148
                     /label=BBa_C0012
     misc_feature    1..1173
                     /label=BBa_J24683
     misc_feature    1186..1189
                     /label=conserved
     RBS             1182..1193
                     /label=BBa_B0034
     CDS             1200..1961
                     /label=YFP (LVA)
     misc_feature    1956..1961
                     /label=2
     misc_feature    1917..1955
                     /label=SsrA
     misc_feature    1441..1441
                     /label=A (Q->L)
     misc_feature    1200..1961
                     /label=BBa_E0032
     misc_feature    1200..1961
                     /label=BBa_E0032
     stem_loop       1981..2024
                     /label=stem_loop
     misc_feature    1970..2049
                     /label=BBa_B0010
     terminator      1970..2049
                     /label=BBa_B0010
     stem_loop       2065..2084
                     /label=T7 TE
     misc_feature    2091..2091
                     /label=stop
     polyA_site      2085..2098
                     /label=polya
     misc_feature    2058..2098
                     /label=BBa_B0012
     terminator      2058..2098
                     /label=BBa_B0012
     misc_feature    1182..2098
                     /label=BBa_J24704
ORIGIN
        1 tcacacagga aacctactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag
       61 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt
      121 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc
      181 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc
      241 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg
      301 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg
      361 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc
      421 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct
      481 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg
      541 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt
      601 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc
      661 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc
      721 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc
      781 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag
      841 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac
      901 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc
      961 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg
     1021 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     1081 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag
     1141 cttaataact ctgatagtgc tagtgtagat ctctactaga gaaagaggag aaatactaga
     1201 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     1261 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     1321 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     1381 tcgtgaccac cttcggctac ggcctgcaat gcttcgcccg ctaccccgac cacatgaagc
     1441 tgcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     1501 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     1561 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     1621 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     1681 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     1741 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     1801 acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     1861 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagaggc
     1921 ctgctgcaaa cgacgaaaac tacgctttag tagcttaata atactagagc caggcatcaa
     1981 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     2041 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//