##gff-version 3
##sequence-region BBa_J24711 1 2098
BBa_J24711	.	engineered_region	1	1173	.	+	0	ID=BBa_J24683;Name=BBa_J24683
BBa_J24711	.	ribosome_entry_site	1	14	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_J24683
BBa_J24711	.	conserved_region	7	10	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_J24711	.	CDS	21	1148	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_J24683
BBa_J24711	.	engineered_region	21	1148	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J24711	.	engineered_tag	1149	1173	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J24711	.	tag	1110	1148	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J24711	.	CDS	21	1148	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J24711	.	engineered_region	1182	2098	.	+	0	ID=BBa_J24704;Name=BBa_J24704
BBa_J24711	.	terminator	1970	2049	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J24704
BBa_J24711	.	stem_loop	1981	2024	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24711	.	engineered_region	1970	2049	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J24711	.	ribosome_entry_site	1182	1193	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24704
BBa_J24711	.	conserved_region	1186	1189	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24711	.	terminator	2058	2098	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J24704
BBa_J24711	.	polyA_site	2085	2098	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24711	.	engineered_region	2058	2098	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24711	.	stem_loop	2065	2084	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J24711	.	stop_codon	2091	2091	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J24711	.	engineered_region	1200	1961	.	+	0	ID=BBa_E0032;Name=BBa_E0032;Parent=BBa_J24704
BBa_J24711	.	stop_codon	1956	1961	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J24711	.	CDS	1200	1961	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J24711	.	sequence_alteration	1441	1441	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J24711	.	engineered_region	1200	1961	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J24711	.	tag	1917	1955	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
>BBa_J24711
tcacacaggaaacctactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctctta
tcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcg
gagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctcca
gtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggt
gtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctg
atcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttg
atgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggt
cgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctgg
cataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaac
aaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaat
gcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctca
tgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaac
tctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaa
tacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcggg
caggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaag
aggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcga
cgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatc
tgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgct
accccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttctt
caaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaag
ggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatca
tggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgc
cgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtcc
gccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcg
gcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttata