LOCUS       BBa_J24712              2097 bp    DNA     linear       25-Nov-2024
DEFINITION  32. M RBS - LacI - S RBS - EYFP(+LVA) - terminator
ACCESSION   BBa_J24712
VERSION     BBa_J24712.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     misc_feature    1109..1147
                     /label=LVA
     CDS             20..1147
                     /label=lacI-LVA
     misc_feature    20..1147
                     /label=BBa_C0012
     misc_feature    1148..1172
                     /label=Help:Barcodes
     CDS             20..1147
                     /label=BBa_C0012
     misc_feature    1..1172
                     /label=BBa_J24684
     misc_feature    1185..1188
                     /label=conserved
     RBS             1181..1192
                     /label=BBa_B0034
     CDS             1199..1960
                     /label=YFP (LVA)
     misc_feature    1955..1960
                     /label=2
     misc_feature    1916..1954
                     /label=SsrA
     misc_feature    1440..1440
                     /label=A (Q->L)
     misc_feature    1199..1960
                     /label=BBa_E0032
     misc_feature    1199..1960
                     /label=BBa_E0032
     stem_loop       1980..2023
                     /label=stem_loop
     misc_feature    1969..2048
                     /label=BBa_B0010
     terminator      1969..2048
                     /label=BBa_B0010
     stem_loop       2064..2083
                     /label=T7 TE
     misc_feature    2090..2090
                     /label=stop
     polyA_site      2084..2097
                     /label=polya
     misc_feature    2057..2097
                     /label=BBa_B0012
     terminator      2057..2097
                     /label=BBa_B0012
     misc_feature    1181..2097
                     /label=BBa_J24704
ORIGIN
        1 tcacacagga aagtactaga tggtgaatgt gaaaccagta acgttatacg atgtcgcaga
       61 gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc
      121 tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg
      181 cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct
      241 ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg
      301 tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt
      361 gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca
      421 ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc
      481 tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt
      541 ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc
      601 tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca
      661 gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca
      721 aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct
      781 gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt
      841 gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca
      901 ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca
      961 ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc
     1021 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     1081 acaggtttcc cgactggaaa gcgggcaggc tgcaaacgac gaaaactacg ctttagtagc
     1141 ttaataactc tgatagtgct agtgtagatc tctactagag aaagaggaga aatactagat
     1201 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1261 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1321 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1381 cgtgaccacc ttcggctacg gcctgcaatg cttcgcccgc taccccgacc acatgaagct
     1441 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     1501 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     1561 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     1621 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     1681 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     1741 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     1801 cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     1861 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagaggcc
     1921 tgctgcaaac gacgaaaact acgctttagt agcttaataa tactagagcc aggcatcaaa
     1981 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     2041 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//