LOCUS       BBa_J24713              2096 bp    DNA     linear       08-Apr-2025
DEFINITION  33. S RBS - LacI - S RBS - EYFP(+LVA) - terminator
ACCESSION   BBa_J24713
VERSION     BBa_J24713.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    1108..1146
                     /label=LVA
     CDS             19..1146
                     /label=lacI-LVA
     misc_feature    19..1146
                     /label=BBa_C0012
     misc_feature    1147..1171
                     /label=Help:Barcodes
     CDS             19..1146
                     /label=BBa_C0012
     misc_feature    1..1171
                     /label=BBa_J24685
     misc_feature    1184..1187
                     /label=conserved
     RBS             1180..1191
                     /label=BBa_B0034
     CDS             1198..1959
                     /label=YFP (LVA)
     misc_feature    1954..1959
                     /label=2
     misc_feature    1915..1953
                     /label=SsrA
     misc_feature    1439..1439
                     /label=A (Q->L)
     misc_feature    1198..1959
                     /label=BBa_E0032
     misc_feature    1198..1959
                     /label=BBa_E0032
     stem_loop       1979..2022
                     /label=stem_loop
     misc_feature    1968..2047
                     /label=BBa_B0010
     terminator      1968..2047
                     /label=BBa_B0010
     stem_loop       2063..2082
                     /label=T7 TE
     misc_feature    2089..2089
                     /label=stop
     polyA_site      2083..2096
                     /label=polya
     misc_feature    2056..2096
                     /label=BBa_B0012
     terminator      2056..2096
                     /label=BBa_B0012
     misc_feature    1180..2096
                     /label=BBa_J24704
ORIGIN
        1 aaagaggaga aatactagat ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag
       61 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct
      121 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc
      181 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg
      241 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt
      301 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg
      361 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag
      421 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct
      481 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg
      541 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct
      601 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag
      661 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa
      721 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg
      781 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg
      841 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag
      901 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag
      961 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg
     1021 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     1081 caggtttccc gactggaaag cgggcaggct gcaaacgacg aaaactacgc tttagtagct
     1141 taataactct gatagtgcta gtgtagatct ctactagaga aagaggagaa atactagatg
     1201 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     1261 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     1321 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     1381 gtgaccacct tcggctacgg cctgcaatgc ttcgcccgct accccgacca catgaagctg
     1441 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     1501 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     1561 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     1621 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     1681 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     1741 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     1801 ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     1861 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagaggcct
     1921 gctgcaaacg acgaaaacta cgctttagta gcttaataat actagagcca ggcatcaaat
     1981 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     2041 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//