##gff-version 3
##sequence-region BBa_J24713 1 2096
BBa_J24713	.	engineered_region	1	1171	.	+	0	ID=BBa_J24685;Name=BBa_J24685
BBa_J24713	.	CDS	19	1146	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_J24685
BBa_J24713	.	engineered_region	19	1146	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J24713	.	engineered_tag	1147	1171	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J24713	.	tag	1108	1146	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J24713	.	CDS	19	1146	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J24713	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24685
BBa_J24713	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24713	.	engineered_region	1180	2096	.	+	0	ID=BBa_J24704;Name=BBa_J24704
BBa_J24713	.	terminator	1968	2047	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J24704
BBa_J24713	.	stem_loop	1979	2022	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24713	.	engineered_region	1968	2047	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J24713	.	ribosome_entry_site	1180	1191	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24704
BBa_J24713	.	conserved_region	1184	1187	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24713	.	terminator	2056	2096	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J24704
BBa_J24713	.	polyA_site	2083	2096	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24713	.	engineered_region	2056	2096	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24713	.	stem_loop	2063	2082	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J24713	.	stop_codon	2089	2089	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J24713	.	engineered_region	1198	1959	.	+	0	ID=BBa_E0032;Name=BBa_E0032;Parent=BBa_J24704
BBa_J24713	.	sequence_alteration	1439	1439	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J24713	.	stop_codon	1954	1959	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J24713	.	CDS	1198	1959	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J24713	.	engineered_region	1198	1959	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J24713	.	tag	1915	1953	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
>BBa_J24713
aaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatc
agaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcgga
gctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagt
ctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgt
cgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgat
cattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgat
gtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcg
cattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggca
taaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaa
accatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgc
gcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatg
ttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactc
tctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaata
cgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggca
ggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagag
gagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacg
taaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctg
caccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctac
cccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttca
aggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaaggg
catcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatg
gccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccg
accactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgc
cctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggc
atggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttata