LOCUS       BBa_J24714              2767 bp    DNA     linear       19-Apr-2025
DEFINITION  34. pLacI=W RBS=AraC=W RBS=TetR=S RBS=mCherry(+LVA)=terminator
ACCESSION   BBa_J24714
VERSION     BBa_J24714.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    215..218
                     /label=conserved
     RBS             209..222
                     /label=BBa_B0031
     CDS             229..1104
                     /label=araC
     misc_feature    1105..1137
                     /label=LVA
     misc_feature    1144..1168
                     /label=Help:Barcodes
     CDS             229..1168
                     /label=BBa_C0080
     misc_feature    1..1168
                     /label=BBa_J24691
     misc_feature    1183..1186
                     /label=conserved
     RBS             1177..1190
                     /label=BBa_B0031
     CDS             1200..1816
                     /label=tetR
     misc_feature    1817..1850
                     /label=SsrA
     misc_feature    1857..1881
                     /label=Help:Barcodes
     CDS             1197..1881
                     /label=BBa_C0040
     misc_feature    1177..1881
                     /label=BBa_J24686
     misc_feature    1894..1897
                     /label=conserved
     RBS             1890..1901
                     /label=BBa_B0034
     CDS             1908..2615
                     /label=mCherry
     misc_feature    2259..2259
                     /label=C->T (removing PstI site)
     misc_feature    2616..2624
                     /label=LVA
     misc_feature    1908..2630
                     /label=BBa_J06505
     stem_loop       2650..2693
                     /label=stem_loop
     misc_feature    2639..2718
                     /label=BBa_B0010
     terminator      2639..2718
                     /label=BBa_B0010
     stem_loop       2734..2753
                     /label=T7 TE
     misc_feature    2760..2760
                     /label=stop
     polyA_site      2754..2767
                     /label=polya
     misc_feature    2727..2767
                     /label=BBa_B0012
     terminator      2727..2767
                     /label=BBa_B0012
     misc_feature    1890..2767
                     /label=BBa_J24702
     misc_feature    1177..2767
                     /label=BBa_J24705
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggaaa cctactagat ggctgaagcg
      241 caaaatgatc ccctgctgcc gggatactcg tttaacgccc atctggtggc gggtttaacg
      301 ccgattgagg ccaatggtta tctcgatttt tttatcgacc gaccgctggg aatgaaaggt
      361 tatattctca atctcaccat tcgcggtcag ggggtggtga aaaatcaggg acgagaattt
      421 gtctgccgac cgggtgatat tttgctgttc ccgccaggag agattcatca ctacggtcgt
      481 catccggagg ctcgcgaatg gtatcaccag tgggtttact ttcgtccgcg cgcctactgg
      541 catgaatggc ttaactggcc gtcaatattt gccaatacgg gtttctttcg cccggatgaa
      601 gcgcaccagc cgcatttcag cgacctgttt gggcaaatca ttaacgccgg gcaaggggaa
      661 gggcgctatt cggagctgct ggcgataaat ctgcttgagc aattgttact gcggcgcatg
      721 gaagcgatta acgagtcgct ccatccaccg atggataatc gggtacgcga ggcttgtcag
      781 tacatcagcg atcacctggc agacagcaat tttgatatcg ccagcgtcgc acagcatgtt
      841 tgcctgtcgc cgtcgcgtct gtcacatctt ttccgccagc agttagggat tagcgtctta
      901 agctggcgcg aggaccaacg catcagccag gcgaagctgc ttttgagcac tacccggatg
      961 cctatcgcca ccgtcggtcg caatgttggt tttgacgatc aactctattt ctcgcgagta
     1021 tttaaaaaat gcaccggggc cagcccgagc gagttccgtg ccggttgtga agaaaaagtg
     1081 aatgatgtag ccgtcaagtt gtcagctgca aacgacgaaa actacgcttt agtagcttaa
     1141 taacactgat agtgctagtg tagatcacta ctagagtcac acaggaaacc tactagatgt
     1201 ccagattaga taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa
     1261 tcgaaggttt aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt
     1321 attggcatgt aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata
     1381 ggcaccatac tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata
     1441 acgctaaaag ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag
     1501 gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc
     1561 aacaaggttt ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt
     1621 taggttgcgt attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta
     1681 ctactgatag tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg
     1741 cagagccagc cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta
     1801 aatgtgaaag tgggtccgct gcaaacgacg aaaactacgc tttagtagct taataacact
     1861 gatagtgcta gtgtagatca ctactagaga aagaggagaa atactagatg gtgagcaagg
     1921 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
     1981 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     2041 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
     2101 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
     2161 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     2221 aggacggcgg cgtggtgacc gtgacccagg actcctcctt gcaggacggc gagttcatct
     2281 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
     2341 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     2401 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
     2461 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
     2521 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     2581 gccactccac cggcggcatg gacgagctgt acaagttagt agcttaataa tactagagcc
     2641 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     2701 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     2761 gtttata
//