##gff-version 3
##sequence-region BBa_J24714 1 2767
BBa_J24714	.	engineered_region	1	1168	.	+	0	ID=BBa_J24691;Name=BBa_J24691
BBa_J24714	.	ribosome_entry_site	209	222	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_J24691
BBa_J24714	.	conserved_region	215	218	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_J24714	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J24691
BBa_J24714	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J24714	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J24714	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J24714	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J24714	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J24714	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J24714	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J24714	.	CDS	229	1168	.	+	0	ID=BBa_C0080;Name=BBa_C0080;Parent=BBa_J24691
BBa_J24714	.	CDS	229	1104	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_J24714	.	engineered_tag	1144	1168	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
BBa_J24714	.	tag	1105	1137	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_J24714	.	engineered_region	1177	2767	.	+	0	ID=BBa_J24705;Name=BBa_J24705
BBa_J24714	.	engineered_region	1177	1881	.	+	0	ID=BBa_J24686;Name=BBa_J24686;Parent=BBa_J24705
BBa_J24714	.	CDS	1197	1881	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_J24686
BBa_J24714	.	engineered_tag	1857	1881	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J24714	.	CDS	1200	1816	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J24714	.	tag	1817	1850	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J24714	.	ribosome_entry_site	1177	1190	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_J24686
BBa_J24714	.	conserved_region	1183	1186	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_J24714	.	engineered_region	1890	2767	.	+	0	ID=BBa_J24702;Name=BBa_J24702;Parent=BBa_J24705
BBa_J24714	.	engineered_region	1908	2630	.	+	0	ID=BBa_J06505;Name=BBa_J06505;Parent=BBa_J24702
BBa_J24714	.	CDS	1908	2615	.	+	0	ID=annotation1585879;Name=mCherry;Parent=BBa_J06505
BBa_J24714	.	sequence_alteration	2259	2259	.	+	0	ID=annotation1585880;Name=C->T (removing PstI site);Parent=BBa_J06505
BBa_J24714	.	tag	2616	2624	.	+	0	ID=annotation1585887;Name=LVA;Parent=BBa_J06505
BBa_J24714	.	terminator	2639	2718	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J24702
BBa_J24714	.	engineered_region	2639	2718	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J24714	.	stem_loop	2650	2693	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24714	.	ribosome_entry_site	1890	1901	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24702
BBa_J24714	.	conserved_region	1894	1897	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24714	.	terminator	2727	2767	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J24702
BBa_J24714	.	polyA_site	2754	2767	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24714	.	engineered_region	2727	2767	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24714	.	stem_loop	2734	2753	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J24714	.	stop_codon	2760	2760	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_J24714
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaacctactagatggctgaa
gcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatg
gttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggt
ggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactac
ggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggctta
actggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgg
gcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactg
cggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcg
atcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatct
tttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagc
actacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaa
aatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagc
tgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtcacacagg
aaacctactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcga
aggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgg
gctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggc
aagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttagg
tacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagag
aatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgcta
aagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaagg
tgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcc
gctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagagg
agaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacat
ggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgcc
aagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaagg
cctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgat
gaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaag
ctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcgga
tgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctga
ggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacc
tcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagc
tgtacaagttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttc
gttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttta
ta