LOCUS       BBa_J24715              2766 bp    DNA     linear       22-Dec-2024
DEFINITION  35. pLacI=W RBS=AraC=M RBS=TetR=S RBS=mCherry(+LVA)=terminator
ACCESSION   BBa_J24715
VERSION     BBa_J24715.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    215..218
                     /label=conserved
     RBS             209..222
                     /label=BBa_B0031
     CDS             229..1104
                     /label=araC
     misc_feature    1105..1137
                     /label=LVA
     misc_feature    1144..1168
                     /label=Help:Barcodes
     CDS             229..1168
                     /label=BBa_C0080
     misc_feature    1..1168
                     /label=BBa_J24691
     RBS             1177..1189
                     /label=RBS-3\Weak
     RBS             1183..1186
                     /label=RBS
     misc_feature    1177..1189
                     /label=BBa_B0032
     RBS             1177..1189
                     /label=BBa_B0032
     CDS             1199..1815
                     /label=tetR
     misc_feature    1816..1849
                     /label=SsrA
     misc_feature    1856..1880
                     /label=Help:Barcodes
     CDS             1196..1880
                     /label=BBa_C0040
     misc_feature    1177..1880
                     /label=BBa_J24687
     misc_feature    1893..1896
                     /label=conserved
     RBS             1889..1900
                     /label=BBa_B0034
     CDS             1907..2614
                     /label=mCherry
     misc_feature    2258..2258
                     /label=C->T (removing PstI site)
     misc_feature    2615..2623
                     /label=LVA
     misc_feature    1907..2629
                     /label=BBa_J06505
     stem_loop       2649..2692
                     /label=stem_loop
     misc_feature    2638..2717
                     /label=BBa_B0010
     terminator      2638..2717
                     /label=BBa_B0010
     stem_loop       2733..2752
                     /label=T7 TE
     misc_feature    2759..2759
                     /label=stop
     polyA_site      2753..2766
                     /label=polya
     misc_feature    2726..2766
                     /label=BBa_B0012
     terminator      2726..2766
                     /label=BBa_B0012
     misc_feature    1889..2766
                     /label=BBa_J24702
     misc_feature    1177..2766
                     /label=BBa_J24706
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggaaa cctactagat ggctgaagcg
      241 caaaatgatc ccctgctgcc gggatactcg tttaacgccc atctggtggc gggtttaacg
      301 ccgattgagg ccaatggtta tctcgatttt tttatcgacc gaccgctggg aatgaaaggt
      361 tatattctca atctcaccat tcgcggtcag ggggtggtga aaaatcaggg acgagaattt
      421 gtctgccgac cgggtgatat tttgctgttc ccgccaggag agattcatca ctacggtcgt
      481 catccggagg ctcgcgaatg gtatcaccag tgggtttact ttcgtccgcg cgcctactgg
      541 catgaatggc ttaactggcc gtcaatattt gccaatacgg gtttctttcg cccggatgaa
      601 gcgcaccagc cgcatttcag cgacctgttt gggcaaatca ttaacgccgg gcaaggggaa
      661 gggcgctatt cggagctgct ggcgataaat ctgcttgagc aattgttact gcggcgcatg
      721 gaagcgatta acgagtcgct ccatccaccg atggataatc gggtacgcga ggcttgtcag
      781 tacatcagcg atcacctggc agacagcaat tttgatatcg ccagcgtcgc acagcatgtt
      841 tgcctgtcgc cgtcgcgtct gtcacatctt ttccgccagc agttagggat tagcgtctta
      901 agctggcgcg aggaccaacg catcagccag gcgaagctgc ttttgagcac tacccggatg
      961 cctatcgcca ccgtcggtcg caatgttggt tttgacgatc aactctattt ctcgcgagta
     1021 tttaaaaaat gcaccggggc cagcccgagc gagttccgtg ccggttgtga agaaaaagtg
     1081 aatgatgtag ccgtcaagtt gtcagctgca aacgacgaaa actacgcttt agtagcttaa
     1141 taacactgat agtgctagtg tagatcacta ctagagtcac acaggaaagt actagatgtc
     1201 cagattagat aaaagtaaag tgattaacag cgcattagag ctgcttaatg aggtcggaat
     1261 cgaaggttta acaacccgta aactcgccca gaagctaggt gtagagcagc ctacattgta
     1321 ttggcatgta aaaaataagc gggctttgct cgacgcctta gccattgaga tgttagatag
     1381 gcaccatact cacttttgcc ctttagaagg ggaaagctgg caagattttt tacgtaataa
     1441 cgctaaaagt tttagatgtg ctttactaag tcatcgcgat ggagcaaaag tacatttagg
     1501 tacacggcct acagaaaaac agtatgaaac tctcgaaaat caattagcct ttttatgcca
     1561 acaaggtttt tcactagaga atgcattata tgcactcagc gctgtggggc attttacttt
     1621 aggttgcgta ttggaagatc aagagcatca agtcgctaaa gaagaaaggg aaacacctac
     1681 tactgatagt atgccgccat tattacgaca agctatcgaa ttatttgatc accaaggtgc
     1741 agagccagcc ttcttattcg gccttgaatt gatcatatgc ggattagaaa aacaacttaa
     1801 atgtgaaagt gggtccgctg caaacgacga aaactacgct ttagtagctt aataacactg
     1861 atagtgctag tgtagatcac tactagagaa agaggagaaa tactagatgg tgagcaaggg
     1921 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     1981 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     2041 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     2101 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     2161 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     2221 ggacggcggc gtggtgaccg tgacccagga ctcctccttg caggacggcg agttcatcta
     2281 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     2341 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     2401 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     2461 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     2521 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     2581 ccactccacc ggcggcatgg acgagctgta caagttagta gcttaataat actagagcca
     2641 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     2701 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     2761 tttata
//