##gff-version 3
##sequence-region BBa_J24718 1 2765
BBa_J24718	.	engineered_region	1	1167	.	+	0	ID=BBa_J24692;Name=BBa_J24692
BBa_J24718	.	CDS	228	1167	.	+	0	ID=BBa_C0080;Name=BBa_C0080;Parent=BBa_J24692
BBa_J24718	.	CDS	228	1103	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_J24718	.	engineered_tag	1143	1167	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
BBa_J24718	.	tag	1104	1136	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_J24718	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J24692
BBa_J24718	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J24718	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J24718	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J24718	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J24718	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J24718	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J24718	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J24718	.	ribosome_entry_site	209	221	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J24692
BBa_J24718	.	ribosome_entry_site	215	218	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J24718	.	ribosome_entry_site	209	221	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J24718	.	engineered_region	209	221	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J24718	.	engineered_region	1176	2765	.	+	0	ID=BBa_J24706;Name=BBa_J24706
BBa_J24718	.	engineered_region	1888	2765	.	+	0	ID=BBa_J24702;Name=BBa_J24702;Parent=BBa_J24706
BBa_J24718	.	ribosome_entry_site	1888	1899	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24702
BBa_J24718	.	conserved_region	1892	1895	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24718	.	terminator	2637	2716	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J24702
BBa_J24718	.	engineered_region	2637	2716	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J24718	.	stem_loop	2648	2691	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24718	.	engineered_region	1906	2628	.	+	0	ID=BBa_J06505;Name=BBa_J06505;Parent=BBa_J24702
BBa_J24718	.	CDS	1906	2613	.	+	0	ID=annotation1585879;Name=mCherry;Parent=BBa_J06505
BBa_J24718	.	sequence_alteration	2257	2257	.	+	0	ID=annotation1585880;Name=C->T (removing PstI site);Parent=BBa_J06505
BBa_J24718	.	tag	2614	2622	.	+	0	ID=annotation1585887;Name=LVA;Parent=BBa_J06505
BBa_J24718	.	terminator	2725	2765	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J24702
BBa_J24718	.	engineered_region	2725	2765	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24718	.	stop_codon	2758	2758	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J24718	.	stem_loop	2732	2751	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J24718	.	polyA_site	2752	2765	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24718	.	engineered_region	1176	1879	.	+	0	ID=BBa_J24687;Name=BBa_J24687;Parent=BBa_J24706
BBa_J24718	.	CDS	1195	1879	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_J24687
BBa_J24718	.	engineered_tag	1855	1879	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J24718	.	tag	1815	1848	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J24718	.	CDS	1198	1814	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J24718	.	ribosome_entry_site	1176	1188	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J24687
BBa_J24718	.	ribosome_entry_site	1182	1185	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J24718	.	ribosome_entry_site	1176	1188	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J24718	.	engineered_region	1176	1188	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
>BBa_J24718
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaagtactagatggctgaag
cgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatgg
ttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtg
gtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacg
gtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaa
ctggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttggg
caaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgc
ggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcga
tcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatctt
ttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagca
ctacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaa
atgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagct
gcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtcacacagga
aagtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaag
gtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggc
tttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaa
gattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggta
cacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaa
tgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaa
gaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtg
cagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgc
tgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggag
aaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatgg
agggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaa
gctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcc
tacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatga
acttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagct
gcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatg
taccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgagg
tcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctc
ccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctg
tacaagttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgt
tttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata